Ampelopsis grossedentata tea alleviating liver fibrosis in BDL-induced mice via gut microbiota and metabolite modulation

npj Science of Food, Journal Year: 2024, Volume and Issue: 8(1)

Published: Nov. 13, 2024

Liver fibrosis (LF) is a common sequela to diverse chronic liver injuries, leading rising rates of cirrhosis and hepatocellular carcinoma (HCC). As the medicinal edible homologous material, traditional teas have exhibited promising applications in clinical management fibrosis. Here, we generated mouse model explore potent therapeutic ability Ampelopsis grossedentata (AG) tea on this condition by multi-omics analysis. The biochemistry results pointed towards mitigated increases ALT, AST, TBIL, ALP triggered BDL AG-treated group. Examination using H&E Sirius Red staining revealed severe inflammation infiltration, amplified fibrosed regions, creation bile ducts, all which were fallout from BDL. Immunohistochemistry findings also implicated noteworthy upregulation HSC activation marker α-smooth muscle actin (α-SMA) collagen I However, these symptoms demonstrated significant improvement group treated with 100 mg/kg AG. Findings Western Blot test corroborated prominent elevation TNF-α, col1a1, α-SMA, TGF-β, instigated BDL, while AG treatment meaningfully modulated proteins. Furthermore, our study underscored potential involvement several microbiota, such as Ruminococcaceae UCG-014, Eubacterium Ruminantium, Ruminococcus 1, Christensenellaceae R-7, Acetatifactor, Dubosiella, Parasutterella, Faecalibaculum, Defluviitaleaceae UCG-011, progression efficacy This investigation shows that during process ameliorating BDL-induced fibrosis, acid derivatives CDCA, TCDCA, 3-DHC, UCA, DCA, among others, play roles. In study, identified non-bile metabolites, Deltarasin, Thr-Ile-Arg, etc., are entailed improving

Language: Английский

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Advances in the treatment of liver injury based on mesenchymal stem cell-derived exosomes

Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)

Published: Dec. 18, 2024

Mesenchymal stem cells (MSCs) have shown a great potential role in treating liver injury. MSCs can promote regeneration by differentiating into hepatocytes, and also secrete exosomes to participate the repair of Increasing evidence has that mesenchymal cell-derived (MSC-EXOs) play an important In this review, biogenesis function characteristics MSC-EXOs were analyzed based on recent research results. are significant injuries such as fibrosis, failure, hepatocellular carcinoma, oxidative stress, lipid steatosis, process regeneration.

Language: Английский

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The regulatory role and mechanism of exosomes in hepatic fibrosis

Frontiers in Pharmacology, Journal Year: 2023, Volume and Issue: 14

Published: Dec. 1, 2023

Globally, the prevalence and fatality rates of liver disorders are on rise. Among chronic conditions, hepatic fibrosis stands out as a central pathological process. Despite this, approved treatments for currently lacking. Exosomes, small extracellular vesicles secreted by various cell types, play significant role in intercellular communication have emerged essential mediators fibrosis. In this regard, review compiles mechanisms through which exosomes regulate fibrosis, encompassing diverse targets signaling pathways. Furthermore, it delves into regulatory impact modulated natural plant-derived, endogenous, synthetic compounds potential therapeutic strategies addressing

Language: Английский

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Anti-fibrotic Potential of WJ-MSC Exosomes in Liver Fibrosis: Mechanistic Insights and Dose-Response Efficacy

Deleted Journal, Journal Year: 2024, Volume and Issue: 23(1)

Published: Oct. 9, 2024

Background: Hepatic fibrosis is a biological response characterized by the accumulation of extracellular matrix (ECM) during wound healing process. stellate cells (HSCs) play pivotal role in fibrogenesis, transitioning from quiescent to myofibroblast cell types and leading excessive ECM production. Platelet-derived growth factor (PDGF), potent mitogen, produced activated HSCs, stimulating proliferation migration. Objectives: This study aims analyze impact Wharton's jelly mesenchymal stem (WJ-MSC)-derived exosomes on HSC activation induced PDGF liver fibrosis. Methods: cells-T6 were treated with PDGF-BB for 24 hours induce activation, followed treatment varying concentrations WJ-MSC-derived (0, 25, 50 µg/mL) another hours. The effects exosome evaluated through flow cytometry, differentiation assays, dynamic light scattering (DLS), transmission electron microscopy (TEM), RT-PCR, western blot analysis. Results: Our yields promising results, highlighting potential therapeutic dose-dependent decrease fibrotic markers such as α-SMA, COLA1, phosphorylated AKT protein PDGF-BB-treated HSC-T6 suggests that WJ-MSC exert an anti-fibrotic effect inhibiting activation. Conclusions: These findings suggest derived WJ-MSCs hold promise targeting key pathways involved fibrogenesis. Further investigation into underlying mechanisms this clinical applications management warranted.

Language: Английский

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Protective effect of rupatadine on testicular ischemia/reperfusion injury in rats: Modulation of IL-6/STAT3, Akt/ mTOR signaling pathways

Toxicology and Applied Pharmacology, Journal Year: 2024, Volume and Issue: 492, P. 117086 - 117086

Published: Sept. 6, 2024

Language: Английский

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