Advances in Anatomic Pathology,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 25, 2024
Cartilage-forming
tumors
are
a
broad
and
diverse
group
of
neoplasms
frequently
affecting
the
skeleton.
Distinguishing
between
members
this
is
important
because
significant
differences
in
treatment
prognosis.
Accurate
diagnosis
can
be
challenging
similarities
their
clinical,
radiographic,
pathologic
features.
Immunohistochemistry
molecular
tools
helpful
select
instances.
Therefore,
careful
evaluation
correlation
these
features
essential
arriving
at
correct
appropriate
patient
management.
This
review
provides
an
overview
current
literature,
emphasizing
diagnosis.
Deleted Journal,
Journal Year:
2024,
Volume and Issue:
6(1)
Published: June 1, 2024
Abstract
The
interior
environment
of
articular
cartilage
in
osteoarthritis
(OA)
presents
substantial
hurdles,
leading
to
the
malfunction
chondrocytes
and
breakdown
collagen
II-enriched
hyaline
matrix.
Despite
this,
most
clinical
treatments
primarily
provide
temporary
relief
from
OA
discomfort
without
arresting
progression.
This
study
aimed
alleviate
by
developing
intra-articular
injectable
dECM-enhanced
hyaluronic
(HE)
microgels.
HE
hydrogel
was
engineered
shaped
into
uniformly
sized
microgels
using
microfluidics
photopolymerization
techniques.
These
provided
a
spatiotemporal
cascade
effect,
facilitating
rapid
release
growth
factors
slower
ECM
macromolecules
proteins.
process
assisted
recovery
chondrocytes’
function,
promoting
cell
proliferation,
matrix
synthesis,
cartilage-specific
gene
expression
vitro.
It
also
effectively
aided
repair
significantly
reduced
severity
OA,
as
demonstrated
radiological
observation,
gross
appearance,
histological/immunohistochemical
staining,
analysis
an
rat
model
vivo.
Collectively,
with
molecules
have
shown
promise
potential
candidate
for
cell-free
therapy
approach.
Graphical
Cells,
Journal Year:
2023,
Volume and Issue:
13(1), P. 40 - 40
Published: Dec. 24, 2023
Background:
The
healing
of
a
bone
injury
is
highly
complex
process
involving
multitude
different
tissue
and
cell
types,
including
immune
cells,
which
play
major
role
in
the
initiation
progression
regeneration.
Methods:
We
histologically
analyzed
spatio-temporal
occurrence
cells
innate
system
(macrophages),
adaptive
(B
T
lymphocytes),
(osteoblasts
osteoclasts)
fracture
area
femoral
osteotomy
over
time.
This
study
was
performed
gap
mouse
model.
also
investigated
two
key
challenges
successful
regeneration:
hypoxia
revascularization.
Results:
Macrophages
were
present
around
throughout
entire
period.
switch
from
initially
pro-inflammatory
M1
macrophages
to
anti-inflammatory
M2
phenotype
coincided
with
revascularization
as
well
appearance
osteoblasts
area.
indicates
that
are
necessary
for
restoration
vessels
they
an
orchestrating
osteoblastogenesis
during
healing.
presence
emphasizes
their
essential
regenerative
processes
exceeds
mere
pathogen
defense.
B
co-localize
consistently
process,
consolidating
crucial
guiding
formation.
These
histological
data
provide,
first
time,
comprehensive
information
about
interrelationships
cellular
network
one
standardized
set
up.
With
this,
overall
picture
interplay
players
scenario
has
been
created.
Conclusions:
A
distribution
factors
driving
at
time
points
decisive
this
process—especially
initial
steps
inflammation
revascularization,
soft
hard
callus
phases—has
visualized.
results
show
cascade
does
not
consist
five
distinct,
consecutive
phases
but
rather
interrelated
continuous
events,
especially
onset
PeerJ,
Journal Year:
2024,
Volume and Issue:
12, P. e17963 - e17963
Published: Sept. 10, 2024
The
role
of
mitochondria
in
the
pathogenesis
osteoarthritis
(OA)
is
significant.
In
this
study,
we
aimed
to
identify
diagnostic
signature
genes
associated
with
OA
from
a
set
mitochondria-related
(MRGs).
First,
gene
expression
profiles
cartilage
GSE114007
and
GSE57218
were
obtained
Gene
Expression
Omnibus.
And
limma
method
was
used
detect
differentially
expressed
(DEGs).
Second,
biological
functions
DEGs
investigated
using
Ontology
(GO)
Kyoto
Encyclopedia
Genes
Genomes
(KEGG)
enrichment
analysis.
Wayne
plots
employed
visualize
mitochondrial
(MDEGs)
OA.
Subsequently,
LASSO
SVM-RFE
algorithms
elucidate
potential
within
MDEGs.
As
result,
GRPEL
MTFP1
identified
as
genes.
Notably,
GRPEL1
exhibited
low
levels
samples
both
experimental
test
group
datasets,
demonstrating
high
efficacy.
Furthermore,
RT-qPCR
analysis
confirmed
reduced
Grpel1
an
vitro
model.
Lastly,
ssGSEA
revealed
alterations
infiltration
abundance
several
immune
cells
tissue,
which
correlation
expression.
Altogether,
study
has
that
novel
significant
indicator
for
by
employing
two
machine
learning
methodologies.
these
findings
provide
fresh
perspectives
on
targeted
therapeutic
interventions
future.
Veterinary Sciences,
Journal Year:
2024,
Volume and Issue:
11(7), P. 283 - 283
Published: June 21, 2024
The
purpose
of
this
study
was
to
explore
and
verify
genes
that
regulate
the
reproductive
traits
Tibetan
pigs
at
mRNA
level.
ovarian
tissues
(TPs)
Yorkshire
(YPs)
were
selected
as
research
objects,
cDNA
libraries
tissue
transcripts
successfully
constructed
by
RNA-Seq
technique.
A
total
651
differentially
expressed
(DEGs)
screened,
including
414
up-regulated
237
down-regulated
genes.
Through
GO
KEGG
enrichment
analysis,
it
found
these
significantly
enriched
in
cell
process,
reproduction,
proliferation,
binding,
catalytic
activity,
well
oxidative
phosphorylation,
endocrine
resistance,
thyroid
hormone,
Notch,
other
signal
transduction
pathways.
Genes
pathways
closely
related
regulation
analyzed
selected,
Advances in Anatomic Pathology,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 25, 2024
Cartilage-forming
tumors
are
a
broad
and
diverse
group
of
neoplasms
frequently
affecting
the
skeleton.
Distinguishing
between
members
this
is
important
because
significant
differences
in
treatment
prognosis.
Accurate
diagnosis
can
be
challenging
similarities
their
clinical,
radiographic,
pathologic
features.
Immunohistochemistry
molecular
tools
helpful
select
instances.
Therefore,
careful
evaluation
correlation
these
features
essential
arriving
at
correct
appropriate
patient
management.
This
review
provides
an
overview
current
literature,
emphasizing
diagnosis.
