Zhili decoction ameliorates ulcerative colitis by modulating gut microbiota and related metabolite, and inhibiting the TLR4/NF-κB/NLRP3 pathway DOI Creative Commons

TAKASHI TAN,

Qin Chen, Ping Chen

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 23, 2024

Ethnopharmacological Importance Zhili decoction (ZLD) is a traditional Chinese medicine prescription for ulcerative colitis (UC). However, the mechanism by which ZLD exerts its therapeutic effects in context of UC remains unclear. Aim study The aim this was to investigate on gut microbiota and related fecal metabolite levels using mouse model UC. In addition, we examined underlying molecular mechanisms responsible these effects. Materials methods major components were detected ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). An integrated approach employing 16S rRNA metabolomics employed assess potential impacts flora abundance diversity, levels, various metabolic pathways. To further treating UC, expression genes proteins RT-qPCR, immunohistochemical staining Western blotting. Results markedly alleviated symptoms inflammatory injury mice with DSS induced notable alterations microbiome, enhanced microbial diversity augmenting Bacteroidota, Christensenella, Lactobacillus , etc., while reducing Firmicutes / Bacteroidota ratio. treatment significantly modified profile It suppressed arachidonic acid pathway promoted butyrate pathway. reduced factors inhibited TLR4/NF-κB/NLRP3 expression. correlation analysis demonstrated close relationship between microbes, metabolites, factors. Conclusion regulating flora, modulating inhibiting

Language: Английский

Traditional Chinese medicine treats ulcerative colitis by regulating gut microbiota, signaling pathway and cytokine: Future novel method option for pharmacotherapy DOI Creative Commons
Tiancheng Wang, Xinyue Liu, Weijie Zhang

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(6), P. e27530 - e27530

Published: March 1, 2024

BackgroundUlcerative colitis (UC) is a chronic non-specific inflammatory disease with intestinal tract as the main site. The pathogenic of UC has not yet been clarified, and multiple mechanisms can lead to pathogenesis UC. Traditional Chinese medicine (TCM) offers an opportunity for treatment. TCM become preferred treatment characteristics targets, pathways high safety. This review attempted summarize (compound prescriptions, single herbs, active ingredients) discussed their based on analyzing UC-related gut microbiota, signaling pathway cytokine. In order provide more systematic diverse reference in prevention UC, theoretical clinical UC.Materials methodsThe information was acquired from different databases, including Web Science, PubMed, CNKI, Wanfang, VIP databases. We then focused recent research progress by TCM. Finally, deficiencies future perspectives are proposed.ResultsModern pharmacological studies have shown that compound prescriptions (strengthening spleen, clearing heat removing dampness, toxin), herbs (replenishing Qi, heat, tonifying blood, etc.), ingredients (alkaloids, polysaccharides, flavonoids, polyphenols, terpenes, etc.) efficiency regulating cytokine.ConclusionsTCM achieve its purpose acting ways, deserves further development this field.

Language: Английский

Citations

9
Ziyuglycoside II attenuated OVX mice bone loss via inflammatory responses and regulation of gut microbiota and SCFAs DOI Creative Commons
Yilin Zhou,

Yingtong Zhang,

Yafei Qian

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 132, P. 112027 - 112027

Published: April 11, 2024

Osteoporosis (OP) is a frequent clinical problem for the elderly. Traditional Chinese Medicine (TCM) has achieved beneficial results in treatment of OP. Ziyuglycoside II (ZGS II) major active compound Sanguisorba officinalis L. that shown anti-inflammation and antioxidation properties, but little information concerning its anti-OP potential available. Our research aims to investigate mechanism ZGS ameliorating bone loss by inflammatory responses regulation gut microbiota short chain fatty acids (SCFAs) ovariectomized (OVX) mice. We predicted mode action on OP through network pharmacology molecular docking, an OVX mouse model was employed validate efficacy. Then we analyzed impact microstructure, levels cytokines pain mediators serum, inflammation colon, intestinal barrier, composition SCFAs feces. Network identified 55 intersecting targets related Of these, IGF1 may be core target, which successfully docked with showed excellent binding ability. vivo alleviated mice, attenuated systemic inflammation, enhanced reduced threshold, modulated abundance involving norank_f__Muribaculaceae Dubosiella, increased content acetic acid propanoic SCFAs. data indicated mice relieving regulating

Language: Английский

Citations

7
Integration of microbiome, metabolomics and transcriptome for in-depth understanding of berberine attenuates AOM/DSS-induced colitis-associated colorectal cancer DOI Open Access
Mengxia Wang, Yan Ma,

Guodong Yu

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 179, P. 117292 - 117292

Published: Aug. 15, 2024

A type of colorectal cancer (CRC)，Colitis-associated (CAC)， is closely associated with chronic inflammation and gut microbiota dysbiosis. Berberine (BBR) has a long history in the treatment intestinal diseases, which been reported to inhibit colitis CRC. However, mechanism its action still unclear. Here, this study aimed explore potential protective effects BBR on azoxymethane (AOM)/dextransulfate sodium (DSS)-induced tumor mice, elucidate molecular mechanisms by microbiota, genes metabolic alterations. The results showed that inhibited improved function mucosal barrier ameliorate AOM/DSS-induced colitis. And significantly reduced development ki-67 expression tissue along promoted apoptosis. Through analysis based 16 S rRNA gene, we found imbalance were characterized an increase beneficial bacteria, for instance Akkermanisa, Lactobacillus, Bacteroides uniformis acidifaciens. In addition, transcriptome regulated colonic epithelial signaling pathway CAC mice particularly tryptophan metabolism Wnt pathway. Notably, resulted enrichment amino acids microbiota-derived SCFA metabolites. summary, our research findings suggest microbiota-amino acid metabolism-Wnt axis plays critical role maintaining homeostasis, may provide new insights into inhibitory colon cancer.

Language: Английский

Citations

6
Berberine‑calcium alginate-coated macrophage membrane-derived nanovesicles for the oral treatment of ulcerative colitis DOI
Huilin Ma, Y. Li, Huan Shi

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 294, P. 139114 - 139114

Published: Jan. 2, 2025

Language: Английский

Citations

0
Perspectives on Berberine and the Regulation of Gut Microbiota: As an Anti-Inflammatory Agent DOI Creative Commons
Quintero Vargas Jael Teresa de Jesús, J.C. Galvez-Ruiz, Adriana Alejandra Márquez Ibarra

et al.

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(2), P. 193 - 193

Published: Jan. 31, 2025

Berberine is a promising agent for modulating the intestinal microbiota, playing crucial role in human health homeostasis. This natural compound promotes growth of beneficial bacteria such as Bacteroides, Bifidobacterium, and Lactobacillus while reducing harmful Escherichia coli. Clinical preclinical studies demonstrate that helps regulate T2D metabolic disorders, improves blood glucose levels during T2D, reduces lipid profile chronic inflammation, especially when combined with probiotics. represents adjuvant therapy inflammatory diseases, particularly due to its multifaceted actions inhibiting proinflammatory cytokines pathways IBS, IBD, UC modulation gut microbiota and/or enhancement integrity epithelial barrier. review establishes basis future treatment protocols berberine fully elucidates mechanisms.

Language: Английский

Citations

0
Tolerogenic dendritic cell-mediated regulatory T cell differentiation by Chinese herbal formulation attenuates colitis progression DOI Creative Commons
Chunhua Huang, Cheng Lyu,

Heung-Lam Mok

et al.

Journal of Advanced Research, Journal Year: 2024, Volume and Issue: unknown

Published: April 1, 2024

Ulcerative colitis (UC) is a chronic inflammatory disease characterized by loss of immune tolerance to luminal antigens and progressive intestinal tissue injury. Thus, the re-establishment crucial for suppressing aberrant responses UC progression. This study aimed investigate mechanisms underlying action CDD-2103 its bioactive compounds in mediating regulation mouse models colitis. Two experimental models, 2,4,6-trinitrobenzene sulfonic acid (TNBS)- T-cell transfer-induced Rag1-/- mice, were used determine effects on Single-cell transcriptome analysis was profile landscape interactions after treatment. Liquid chromatography-mass spectrometry (LC-MS) analyze major components interacting with lymphoid cells. A primary cell co-culture system confirm component. dose-dependently suppresses progression induced chemicals or T transplantation mice. The effect primarily attributable an increase de novo generation regulatory cells (Tregs) lamina propria (LP). transcriptomic revealed that treatment increased number tolerogenic dendritic (DCs). Mechanistically, promoted DCs accumulation function upregulating several genes electron transport chain related oxidative phosphorylation, leading differentiation Tregs. Further LC-MS identified CDD-2103, particularly those distributed within mesenteric lymph nodes Subsequent studies palmatine berberine bone marrow-derived (BMDC)-mediated Treg differentiation. Overall, our demonstrated clinically beneficial based induction tolerance. In addition, this supports as potential chemical entities modulate

Language: Английский

Citations

2
Gut microbiota-derived tryptophan metabolites regulated by Wuji Wan to attenuate colitis through AhR signaling activation DOI Creative Commons
Wanghui Jing,

Sijing Dong,

Yinyue Xu

et al.

Acta Pharmaceutica Sinica B, Journal Year: 2024, Volume and Issue: 15(1), P. 205 - 223

Published: Nov. 22, 2024

Disruption of the intestinal mucosal barrier caused by gut dysbiosis and metabolic imbalance is underlying pathology inflammatory bowel disease (IBD). Traditional Chinese medicine Wuji Wan (WJW) commonly used to treat digestive system disorders showed therapeutic potential for IBD. In this interdisciplinary study, we aim investigate pharmacological effects WJW against experimental colitis combining functional metabolomics gut-microbiota sequencing techniques. Treatment with altered profile microbiota notably increased abundance Lactobacillus, thereby facilitating conversion tryptophan into indole-3-acetic acid (IAA) indoleacrylic (IA). These indole derivatives activated aryl hydrocarbon receptor (AhR) pathway, which reduced colonic inflammation restored expression proteins. Interestingly, beneficial on function improvement metabolism were disappeared in absence microbiota. Finally, pre-treatment AhR antagonist CH-223191 confirmed essential role IAA-mediated activation WJW. Overall, enhanced a murine model modulating Lactobacillus-IAA-AhR signaling pathway. This study provides novel insights pathogenesis presents an effective preventive approach

Language: Английский

Citations

2
Berberine Alleviates Ulcerative Colitis Through Targeting Irgm1 for Anti-Inflammatory Effects DOI

Guibing Meng,

Pengyan Li, Xuemei Du

et al.

Published: Jan. 1, 2024

BackgroundBerberine (BBR), found in Coptidis Rhizoma, is a potential treatment for ulcerative colitis (UC) due to its anti-inflammatory properties. However, specific targets and mechanisms UC are unclear.PurposeThe aim of this study was identify BBR's target mode action treatment.MethodsThe model with dampness-heat syndrome induced mice using dextran sulfate sodium (DSS), high-fat, high-sugar diet, hot, humid environment. The therapeutic effects Rhizoma extract BBR were assessed. Anti-inflammatory proteins identified stable isotope labeling by amino acids cell culture-activity-based protein profiling (SILAC-ABPP) an LPS-induced RAW264.7 inflammation model. Molecular docking, drug affinity responsive stability (DARTS), molecular dynamics simulation, cellular thermal shift assay (CETSA), biological layer interference measurement (BLI) employed the interaction between targets. Lentiviral transfection used knock down investigate mechanism.ResultsCoptidis significantly improved reducing enhancing intestinal barrier expression. SILAC-ABPP IRGM1 as target, overexpression cells reduced treatment. also decreased inflammatory cytokines inhibiting PI3K/AKT/mTOR pathway. Knockdown weakened on cytokine expression pathway regulation.ConclusionBBR effectively treats targeting inhibit pathway, suggesting agent UC.

Language: Английский

Citations

0
Berberine-loaded PLGA nanoparticles alleviate ulcerative colitis by targeting IL-6/IL-6R axis DOI Creative Commons
Chao Liu, Qiming Gong,

Wanning Liu

et al.

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: Oct. 24, 2024

The present study aims to develop a nano-delivery system that encapsulates berberine (BBR) into PLGA-based nanoparticles (BPL-NPs), treat ulcerative colitis (UC). Furthermore, the therapeutic efficacy and molecular targeting mechanisms of BPL-NPs in management UC are thoroughly examined. Emulsion solvent-driven methods were used self-assemble BBR PLGA nanoparticles, resulting development (BPL-NPs). effectiveness was evaluated using dextran sulfate sodium (DSS)-induced model mice lipopolysaccharide (LPS)-induced inflammation THP-1 macrophages. interaction between Mφs NCM-460 cells investigated co-culture system. ability treatment validated through vitro as well vivo experiments. demonstrated particle size 184 ± 22.4 nm, enhanced dispersibility deionized water, notable encapsulation efficiency 31.1 0.2%. use clearly improved clinical symptoms pathological changes associated with while also ensuring minimal toxicity. In addition, intestinal epithelial cell apoptosis function barrier by inhibiting M1 infiltration IL-6 signaling pathway UC. found selectively target IL-6/IL-6R axis during Mφs-induced NCM460 cells. confirmed harbor anti-inflammatory effects both vivo, along water solubility bioactivity. precise mechanism which exerted UC, studies.

Language: Английский

Citations

0
Research Progress of Berberine in Treatment of Intestinal Diseases DOI

奇梦 庞

Advances in Clinical Medicine, Journal Year: 2024, Volume and Issue: 14(11), P. 15 - 20

Published: Jan. 1, 2024

Language: Английский

Citations

0