Pomegranate flower polysaccharide improves mastitis in mice by regulating intestinal flora and restoring the blood-milk barrier DOI Creative Commons
Jianlong Li,

Wen Yin,

Yuan Liang

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 15, 2024

This study explored the inhibitory effect of pomegranate flower polysaccharide (PFPS) on mastitis through in vitro and vivo models. PFPS is a new type isolated extracted from flowers. The result revealed that consists GalA, Ara, Gal, residues consist 1,4-GalpA, 1,4-Galp, 1,3,6-Galp, which contain HG-type RG-I-type pectin structural domains. In studies showed could inhibit LPS-enhanced phagocytosis RAW 264.7 cells release IL-1β, IL-10, TNF-α. , improved xylene-induced mouse ear swelling carrageenan-induced paw edema by inhibiting inflammatory factors. model, significantly LPS-induced inflammation oxidative stress mammary tissue. Intestinal flora sequencing results effectively regulate intestinal mice, reduce relative abundance pathogenic bacteria Oscillospira AF12 increase probiotics Blautia Parabacteroides Allobaculum Clostridiaceae_Clostridium . Therefore, ultimately played role preventing regulating further improving blood-milk barrier. provides scientific basis for as potential candidate drug treatment mastitis.

Language: Английский

Hesperetin Alleviated Experimental Colitis via Regulating Ferroptosis and Gut Microbiota DOI Open Access
Jinzhi Wang, Yuanyuan Yao, Ting Yao

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(14), P. 2343 - 2343

Published: July 19, 2024

Hesperetin (HT) is a type of citrus flavonoid with various pharmacological activities, including anti-tumor, anti-inflammation, antioxidant, and neuroprotective properties. However, the role mechanism HT in ulcerative colitis (UC) have been rarely studied. Our study aimed to uncover beneficial effects its detailed UC. Experimental was induced by 2.5% dextran sodium sulfate (DSS) for seven days. ameliorated DSS-induced mice, showing marked improvement weight loss, colon length, colonic pathological severity, levels TNFα IL6 serum. A combination informatics, network pharmacology, molecular docking identified eight key targets multi-pathways influenced As highlight, experimental validation demonstrated that PTGS2, marker ferroptosis, along other indicators ferroptosis (such as ACSL4, Gpx4, lipid peroxidation), were regulated vivo vitro. Additionally, supplement increased diversity gut microbiota, decreased relative abundance Proteobacteria Gammaproteobacteria, restored bacteria (Lachnospiraceae_NK4A136_group Prevotellaceae_UCG-001). In conclusion, an effective nutritional against suppressing modulating microbiota.

Language: Английский

Citations

9
Integrating the milk microbiome signatures in mastitis: milk-omics and functional implications DOI Creative Commons
Rine Christopher Reuben, Cármen Torres

World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(2)

Published: Jan. 18, 2025

Mammalian milk contains a variety of complex bioactive and nutritional components microorganisms. These microorganisms have diverse compositions functional roles that impact host health disease pathophysiology, especially mastitis. The advent use high throughput omics technologies, including metagenomics, metatranscriptomics, metaproteomics, metametabolomics, as well culturomics in microbiome studies suggest strong relationships between phenotype signatures While single undoubtedly contributed to our current understanding mastitis, they often provide limited information, targeting only biological viewpoint which is insufficient system-wide information necessary for elucidating the footprints molecular mechanisms driving mastitis dysbiosis. Therefore, integrating multi-omics approach research could generate new knowledge, improve structural ecosystem, insights sustainable control management.

Language: Английский

Citations

1
Puerarin Delays Mammary Gland Aging by Regulating Gut Microbiota and Inhibiting the p38MAPK Signaling Pathway DOI
Shu Liu, Huijie Hu, Meng Zhang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(19), P. 10879 - 10896

Published: April 30, 2024

Mammary gland aging is one of the most important problems faced by humans and animals. How to delay mammary particularly important. Puerarin a kind isoflavone substance extracted from Pueraria lobata, which has anti-inflammatory, antioxidant, other pharmacological effects. However, role puerarin in delaying lipopolysaccharide (LPS)-induced its underlying mechanism remains unclear. On hand, we found that could significantly downregulate expression senescence-associated secretory phenotype (SASP) age-related indicators (SA-β-gal, p53, p21, p16) glands mice. In addition, mainly inhibited p38MAPK signaling pathway repair mitochondrial damage aging. also cellular senescence mice epithelial cells (mMECs) targeting gut microbiota promoting secretion metabolites. conclusion, not only directly act on mMECs but regulate microbiota, thus, playing gland. Based above findings, have discovered new for

Language: Английский

Citations

4
Encapsulated hesperetin modulates inflammatory responses in an in vitro intestinal immune co-culture model DOI Creative Commons
Xiangnan Meng,

Monic M. M. Tomassen,

Christos Fryganas

et al.

Food Research International, Journal Year: 2025, Volume and Issue: 204, P. 115916 - 115916

Published: Feb. 4, 2025

Language: Английский

Citations

0
The role and molecular mechanisms of amino acids in enhancing mammary gland milk synthesis – a comprehensive review DOI
Yuan Qi, Yanshun Xu

Food Research International, Journal Year: 2025, Volume and Issue: unknown, P. 116355 - 116355

Published: April 1, 2025

Language: Английский

Citations

0
Angelica sinensis Polysaccharide Alleviates Staphylococcus aureus-Induced Mastitis by Regulating The Intestinal Flora and Gut Metabolites DOI
Xin Ran, Yutao Li, Weiwei Guo

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 25, 2024

The modulation of intestinal flora by various polysaccharides has been shown to mitigate disease progression. Recent research reveals a significant link between and the progression mastitis. This study demonstrates that oral administration

Language: Английский

Citations

3
Hordenine Alleviates Lipopolysaccharide-Induced Mastitis by Suppressing Inflammation and Oxidative Stress, Modulating Intestinal Microbiota, and Preserving the Blood–Milk Barrier DOI

Yachun Xie,

Xinyi Li, Dianwen Xu

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 17, 2024

Mastitis is a common mammalian disease occurring in the mammary tissue and poses major threat to agriculture dairy industry. Hordenine (HOR), phenylethylamine alkaloid naturally extracted from malt, has various pharmacological effects, but its role mastitis unknown. The aim of this study was investigate HOR underlying mechanism lipopolysaccharide (LPS)-induced inflammatory response model mouse epithelial cells (EpH4-Ev) model. experimental results showed that attenuated LPS-induced damage (from 3.75 ± 0.25 1.75 0.25) restored integrity blood-milk barrier. Further mechanistic studies revealed inhibited overactivation TLR4-MAPK/NF-κB signaling pathway activated AMPK/Nrf2/HO-1 pathway. Additionally, altered composition intestinal microbiota mice, ultimately reducing extent injury 3.33 0.33 0.67 0.33) upregulating expression tight junction proteins (ZO-1, occludin, claudin-3). findings provide theoretical basis rational use for prevention treatment maintenance gland health.

Language: Английский

Citations

2
Astragalus polysaccharide alleviates mastitis disrupted by Staphylococcus aureus infection by regulating gut microbiota and SCFAs metabolism DOI
Kefei Li, Xin Ran,

Jiaxi Han

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 286, P. 138422 - 138422

Published: Dec. 7, 2024

Language: Английский

Citations

2
Allantoin ameliorates dopaminergic neuronal damage in MPTP-induced Parkinson's disease mice via regulating oxidative damage, inflammation, and gut microbiota disorder DOI

S.-r. Yang,

Xiaojia Sun,

Dianfeng Liu

et al.

Food & Function, Journal Year: 2024, Volume and Issue: 15(18), P. 9390 - 9408

Published: Jan. 1, 2024

Parkinson's disease (PD) is a chronic progressive neurodegenerative that often occurs in older people. Neuroinflammation and oxidative stress are important factors the development of PD. Gastrointestinal dysfunction most common non-motor symptom, inflammation gut, which activates gut-brain axis, maybe pathogenic factor. Previous studies have attributed anti-inflammatory antioxidant effects to Allantoin, but its function mechanism action PD unclear. This study aimed investigate effect Allantoin on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mice. Our results showed administration ameliorated motor neuronal damage mice injected with MPTP by inhibiting neuroinflammation damage. Mechanistic suppresses inflammatory responses overactivation NF-κB MAPK signaling pathways, as well regulating AKT/Nrf2/HO-1 pathway. Notably, also restored intestinal barrier modulating gut microbiota improving capacities alleviate MPTP-induced deficits. In conclusion, present shows attenuated neurodegeneration composition microbiome.

Language: Английский

Citations

1
Pomegranate flower polysaccharide improves mastitis in mice by regulating intestinal flora and restoring the blood-milk barrier DOI Creative Commons
Jianlong Li,

Wen Yin,

Yuan Liang

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 15, 2024

This study explored the inhibitory effect of pomegranate flower polysaccharide (PFPS) on mastitis through in vitro and vivo models. PFPS is a new type isolated extracted from flowers. The result revealed that consists GalA, Ara, Gal, residues consist 1,4-GalpA, 1,4-Galp, 1,3,6-Galp, which contain HG-type RG-I-type pectin structural domains. In studies showed could inhibit LPS-enhanced phagocytosis RAW 264.7 cells release IL-1β, IL-10, TNF-α. , improved xylene-induced mouse ear swelling carrageenan-induced paw edema by inhibiting inflammatory factors. model, significantly LPS-induced inflammation oxidative stress mammary tissue. Intestinal flora sequencing results effectively regulate intestinal mice, reduce relative abundance pathogenic bacteria Oscillospira AF12 increase probiotics Blautia Parabacteroides Allobaculum Clostridiaceae_Clostridium . Therefore, ultimately played role preventing regulating further improving blood-milk barrier. provides scientific basis for as potential candidate drug treatment mastitis.

Language: Английский

Citations

0