Post-harvest motility and morphological changes of spermatozoa following caudal epididymal recovery in farmed common eland (Tragelaphus oryx) bulls
Reproductive Biology,
Journal Year:
2025,
Volume and Issue:
25(2), P. 100997 - 100997
Published: Feb. 21, 2025
Language: Английский
A multivariate model for the prediction of pregnancy following laparoscopic artificial insemination of sheep
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Nov. 11, 2024
The
causes
of
variation
in
the
success
laparoscopic
artificial
insemination
(AI)
sheep
are
not
well
understood.
As
such,
this
study
incorporated
contributions
multiple
male
and
female
factors
relevant
to
AI
into
a
comprehensive
prediction
model
for
pregnancy
success.
Data
from
Merino
ewes
(N
=
30
254)
including
age,
uterine
tone
(1;
pale/flaccid-5;
turgid/pink),
intra-abdominal
fat
little
no
present-5;
high
fat),
time
sire
used,
were
recorded
during
AI.
A
subset
semen
per
388)
was
thawed
assessed
volume,
subjective
motility,
sperm
concentration,
morphology.
Sperm
motility
(CASA),
viability
acrosome
integrity
(FITC-PNA/PI),
membrane
fluidity
(M540/Yo-Pro),
mitochondrial
superoxide
production
(Mitosox
Red/Sytox
Green),
lipid
peroxidation
(Bodipy
C11),
level
intracellular
reactive
oxygen
species
(H
Language: Английский
RhoA improves cryopreservation of rooster sperm through the Rho/RhoA-associated kinase/cofilin pathway
Nuo Heng,
No information about this author
Zhixian Zhao,
No information about this author
Yong Guo
No information about this author
et al.
Poultry Science,
Journal Year:
2022,
Volume and Issue:
101(10), P. 102051 - 102051
Published: July 14, 2022
Cryopreservation
of
rooster
sperm
leads
to
relatively
low
semen
quality
due
cytoskeletal
damage
during
the
freeze-thawing
process.
This
study
aimed
explore
how
addition
RhoA
recombinant
protein
affected
viability
and
subcellular
structure
after
elucidated
molecular
mechanisms
cryopreservation.
Semen
acrosome
integrity
testing
revealed
that
0.5
μg/mL
cryoprotectant
fluid
significantly
increased
motility,
survival
rate,
linearity,
straight-line
velocity,
(P
<
0.05).
Ultrastructure
analysis
cryopreserved
showed
structural
plasma
membrane,
nuclear
tail.
However,
compared
control,
these
changes
were
reduced
upon
cryoprotective
Western
blotting
expression
Rho/RhoA-associated
kinase
p-cofilin
was
increased,
cofilin
decreased
cryopreservation
with
protein.
Treatment
Y-27632,
a
ROCK
antagonist,
suppressed
quality,
integrity,
ultrastructure
integrity.
In
summary,
we
have
demonstrated
effect
in
spermatozoa
involving
Rho/ROCK
pathway
freeze-thawing.
Furthermore,
improved
homeostasis
via
pathway.
may
regulate
dynamic
reorganization
actin
cytoskeleton
by
regulating
phosphorylation.
Language: Английский
Assessment of the morphometry of heads of normal sperm and sperm with the Dag defect in the semen of Duroc boars
Journal of Veterinary Research,
Journal Year:
2021,
Volume and Issue:
65(2), P. 239 - 244
Published: April 23, 2021
Abstract
Introduction
The
Dag
defect
is
one
of
the
primary
morphological
defects
in
sperm
correlating
with
reduced
fertility.
This
found
spermatozoa
many
livestock
species.
aim
study
was
to
assess
morphometry
heads
normal
and
semen
Duroc
breeding
boars.
Material
Methods
Sperm
morphology
examined
ten
ejaculates
each
from
12
In
total,
3,600
morphologically
838
were
evaluated.
area,
perimeter,
length
width
head
measured
these
basic
morphometric
parameters
used
calculate
four
additional
shape
indices
characterising
head,
i.e.
ellipticity,
elongation,
roughness
regularity.
Results
this
had
markedly
smaller
heads,
0.32
μm
shorter
0.19
narrower
than
structure.
also
a
1.1μm
perimeter
2.5
2
surface
area
sperm.
Conclusions
boar
irrespective
age
individual.
It
affects
head.
Language: Английский
A preliminary study of Lasia spinosa Thw. feed supplementation improving semen quality of subfertile purebred boars
The Thai Journal of Veterinary Medicine,
Journal Year:
2024,
Volume and Issue:
54(1), P. 63 - 67
Published: Aug. 23, 2024
Poor
semen
quality
is
a
significant
issue
among
imported
purebred
boars
in
Thailand,
affecting
both
itself
and
reducing
productivity
at
AI
stations.
The
present
study
investigated
the
effects
of
dietary
supplementation
with
Lasia
spinosa
Thw.
(LST)
on
improving
boar
quality.
A
group
experiencing
poor
were
monitored,
three
selected
due
to
their
persistent
low
profiles
for
experiment.
Dried
LST
powder
was
prepared
small
bags
dosage
4
g
per
100
kg
body
weight
added
regular
feed
daily
oral
administration
over
an
8-week
experiment
period.
Semen
collected
from
one
week
before
treatment
initiation
end
Each
sample
assessed
volume,
sperm
concentration,
motility,
viability,
acrosome
integrity,
mitochondrial
activity,
morphology.
Dietary
significantly
improved
volume
(P
=
0.03),
motility
viability
integrity
0.03).
percentage
normal
head
morphology
remained
unchanged.
However,
major
minor
defects
reduced
In
conclusion,
enhances
subfertile
feasible
intervention
swine
farms.
Language: Английский
Association between sperm morphology and sperm count of boar semen
S. Kondracki,
No information about this author
Konrad Górski,
No information about this author
Maria Iwanina
No information about this author
et al.
Polish Journal of Veterinary Sciences,
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 12, 2023
The
number
of
spermatozoa
in
the
ejaculate
is
important
for
its
quality
and
that
sperm
contained
it.
ejaculated
also
associated
with
dimensions.
aim
this
study
was
to
assess
morphological
structure
frequency
abnormalities
on
ejaculation
performance
boars,
measured
as
total
per
ejaculate.
conducted
using
648
ejaculates
collected
from
31
Large
White
boars
30
Landrace
boars.
All
were
analysed
basic
physical
characteristics
abnormalities.
In
addition,
morphometric
measurements
made
used
calculate
their
shape
indexes.
As
a
result
our
it
noted
most
have
shorter
heads
smaller
area
than
small
or
intermediate
spermatozoa.
produce
semen
better
quality,
percentage
major
abnormalities,
differences
between
breeds
increase
larger
longer
flagella
dimensions
decrease
increases.
shown
influence
sperm.
effect
morphology
depends
breed
male.
Language: Английский
A multivariate model for the prediction of pregnancy following laparoscopic artificial insemination of sheep.
Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 9, 2024
Abstract
The
causes
of
variation
in
the
success
laparoscopic
artificial
insemination
(AI)
sheep
are
not
well
understood.
As
such,
this
study
incorporated
contributions
multiple
male
and
female
factors
relevant
to
AI
into
a
comprehensive
prediction
model
for
pregnancy
success.
Data
from
Merino
ewes
(N
=
30
254)
including
age,
uterine
tone
(1;
pale/flaccid-5;
turgid/pink),
intra-abdominal
fat
little
no
present-5;
high
fat),
time
sire
used,
were
recorded
during
AI.
A
subset
semen
per
388)
was
thawed
assessed
volume,
subjective
motility,
sperm
concentration,
morphology.
Sperm
motility
(CASA),
viability
acrosome
integrity
(FITC-PNA/PI),
membrane
fluidity
(M540/Yo-Pro),
mitochondrial
superoxide
production
(Mitosox
Red/Sytox
Green),
lipid
peroxidation
(Bodipy
C11),
level
intracellular
reactive
oxygen
species
(H
2DCFDA)
DNA
fragmentation
(Acridine
Orange)
also
0,
3
6h
post-thaw.
Logistic
binomial
regression
revealed
concentration
(P
<
0.001),
CASA
parameters
at
0h
(PCA3;
P
0.03),
viable
intact
0.02),
abnormal
morphology
0.001)
0.03)
influenced
likelihood
pregnancy.
Results
generated
will
help
standardise
pre-screening
selection
prior
breeding
programs,
reducing
Language: Английский
Effects of breed and ejaculate volume on sperm morphology and semen parameters of boars
Krzysztof Górski,
No information about this author
S. Kondracki,
No information about this author
Maria Iwanina
No information about this author
et al.
Animal Science Journal,
Journal Year:
2021,
Volume and Issue:
92(1)
Published: Jan. 1, 2021
Abstract
The
aim
of
the
study
was
to
determine
relation
between
semen
quality,
frequency
sperm
defects,
dimensions
and
shape,
ejaculate
volume
Large
White
Landrace
boars.
A
total
648
ejaculates
collected
from
31
30
boars
were
divided
into
three
groups
according
criterion
volume.
In
this
produced
with
higher
volume,
concentration,
numbers
spermatozoa
than
also
showed
a
lower
morphological
abnormalities
(
P
<
0.05).
had
larger
heads,
which
by
0.15
μm
longer,
perimeter
area
boar
longer
flagellum
generally
2.07
Significant
differences
found
in
shape
two
breeds
more
elongated
ratio
head
size
length
Sperm
low
shorter
greater
length/flagellum
medium‐
high‐volume
Language: Английский