Cells,
Journal Year:
2018,
Volume and Issue:
7(12), P. 268 - 268
Published: Dec. 12, 2018
Skin
undergoes
continuous
renewal
throughout
an
individual’s
lifetime
relying
on
stem
cell
functionality.
However,
a
decline
of
the
skin
regenerative
potential
occurs
with
age.
The
accumulation
senescent
cells
over
time
probably
reduces
tissue
regeneration
and
contributes
to
aging.
Keratinocytes
dermal
fibroblasts
undergo
senescence
in
response
several
intrinsic
or
extrinsic
stresses,
including
telomere
shortening,
overproduction
reactive
oxygen
species,
diet,
sunlight
exposure.
Epigenetic
mechanisms
directly
regulate
homeostasis
regeneration,
but
they
also
mark
natural
pathological
aging
processes.
Progeroid
syndromes
represent
group
clinical
genetically
heterogeneous
pathologies
characterized
by
accelerated
various
tissues
organs,
skin.
from
progeroid
patients
display
molecular
hallmarks
that
mimic
those
associated
naturally
occurring
Thus,
investigations
strongly
contribute
disclose
causal
underlie
process.
In
present
review,
we
discuss
role
epigenetic
pathways
regulation
during
physiologic
premature
Stem Cell Research & Therapy,
Journal Year:
2019,
Volume and Issue:
10(1)
Published: Feb. 26, 2019
In
recent
years,
stem
cell
therapy
has
become
a
very
promising
and
advanced
scientific
research
topic.
The
development
of
treatment
methods
evoked
great
expectations.
This
paper
is
review
focused
on
the
discovery
different
cells
potential
therapies
based
these
cells.
genesis
followed
by
laboratory
steps
controlled
culturing
derivation.
Quality
control
teratoma
formation
assays
are
important
procedures
in
assessing
properties
tested.
Derivation
utilization
media
crucial
to
set
proper
environmental
conditions
for
differentiation.
Among
many
types
tissue
applications,
use
graphene
scaffolds
extracellular
vesicle-based
require
attention
due
their
versatility.
summarized
challenges
that
must
overcome
be
accepted
worldwide.
A
wide
variety
possibilities
makes
this
cutting
edge
turning
point
modern
medicine,
providing
hope
untreatable
diseases.
Communications Biology,
Journal Year:
2020,
Volume and Issue:
3(1)
Published: April 23, 2020
Abstract
Fibroblasts
are
an
essential
cell
population
for
human
skin
architecture
and
function.
While
fibroblast
heterogeneity
is
well
established,
this
phenomenon
has
not
been
analyzed
systematically
yet.
We
have
used
single-cell
RNA
sequencing
to
analyze
the
transcriptomes
of
more
than
5,000
fibroblasts
from
a
sun-protected
area
in
healthy
donors.
Our
results
define
four
main
subpopulations
that
can
be
spatially
localized
show
differential
secretory,
mesenchymal
pro-inflammatory
functional
annotations.
Importantly,
we
found
‘priming’
becomes
reduced
with
age.
also
aging
causes
substantial
reduction
predicted
interactions
between
dermal
other
cells,
including
undifferentiated
keratinocytes
at
dermal-epidermal
junction.
work
thus
provides
evidence
specialization
identifies
partial
loss
cellular
identity
as
important
age-related
change
dermis.
These
findings
implications
understanding
its
associated
phenotypes.
International Journal of Molecular Sciences,
Journal Year:
2018,
Volume and Issue:
19(6), P. 1715 - 1715
Published: June 9, 2018
Stem
cells
and
their
paracrine
factors
have
emerged
as
a
resource
for
regenerative
medicine.
Many
studies
shown
the
beneficial
effects
of
secreted
from
adult
stem
cells,
such
exosomes,
on
skin
aging.
However,
to
date,
few
reports
demonstrated
use
exosomes
derived
human
pluripotent
treatment
In
this
study,
we
collected
conditioned
medium
induced
(iPSCs)
investigated
effect
aged
dermal
fibroblasts
(HDFs).
Cell
proliferation
viability
were
determined
by
an
MTT
assay
cell
migration
capacity
was
scratch
wound
transwell
assay.
To
induce
photoaging
natural
senescence,
HDFs
irradiated
UVB
(315
nm)
subcultured
over
30
passages,
respectively.
The
expression
level
certain
mRNAs
evaluated
quantitative
real-time
PCR
(qPCR).
Senescence-associated-β-galactosidase
(SA-β-Gal)
activity
assessed
marker
senescence.
As
result,
found
that
iPSCs
(iPSCs-Exo)
stimulated
under
normal
conditions.
Pretreatment
with
iPSCs-Exo
inhibited
damages
overexpression
matrix-degrading
enzymes
(MMP-1/3)
caused
irradiation.
also
increased
collagen
type
I
in
photo-aged
HDFs.
addition,
significantly
reduced
SA-β-Gal
MMP-1/3
restored
senescent
Taken
together,
it
is
anticipated
these
results
suggest
therapeutic
potential
Genome biology,
Journal Year:
2018,
Volume and Issue:
19(1)
Published: Dec. 1, 2018
Biomarkers
of
aging
can
be
used
to
assess
the
health
individuals
and
study
age-related
diseases.
We
generate
a
large
dataset
genome-wide
RNA-seq
profiles
human
dermal
fibroblasts
from
133
people
aged
1
94
years
old
test
whether
signatures
are
encoded
within
transcriptome.
develop
an
ensemble
machine
learning
method
that
predicts
age
median
error
4
years,
outperforming
previous
methods
predict
age.
The
was
further
validated
by
testing
it
on
ten
progeria
patients,
our
is
only
one
accelerated
in
these
patients.
The FASEB Journal,
Journal Year:
2020,
Volume and Issue:
34(3), P. 3519 - 3536
Published: Feb. 10, 2020
The
inherent
plasticity
and
resiliency
of
fibroblasts
make
this
cell
type
a
conventional
tool
for
basic
research.
But
where
do
they
come
from,
are
all
the
same,
how
function
in
disease?
first
fibroblast
lineages
mammalian
development
emerge
from
ooze
primary
mesenchyme
during
gastrulation.
They
cells
that
efficiently
create
negotiate
extracellular
matrix
mesoderm
order
to
migrate
meet
their
developmental
fate.
Mature
epithelial
tissues
live
interstitial
spaces
between
basement
membranes
spatially
delimit
complex
organ
structures.
While
resident
healthy
is
largely
conjecture,
accumulation
pathologic
lesions
offers
insight
into
biologic
mechanisms
control
function;
poised
coordinate
fibrogenesis
tissue
injury,
neoplasia,
aging.
Here,
we
examine
origin
fibroblasts,
molecular
functional
definitions,
epigenetic
underlying
identity
activation,
evolution
immune
regulatory
functions.
These
topics
reviewed
through
lens
fate
mapping
using
genetically
engineered
mouse
models
perspective
single-cell
RNA
sequencing.
Recent
observations
suggest
dynamic
heterogeneous
functions
underscore
signatures
utility
injured
tissues.
