Sialylation in the gut: From mucosal protection to disease pathogenesis

Carbohydrate Polymers, Journal Year: 2024, Volume and Issue: 343, P. 122471 - 122471

Published: July 9, 2024

Language: Английский

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Roles of Siglecs in neurodegenerative diseases

Molecular Aspects of Medicine, Journal Year: 2022, Volume and Issue: 90, P. 101141 - 101141

Published: Sept. 9, 2022

Language: Английский

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MicroRNA:Siglec crosstalk in cancer progression

Current Opinion in Chemical Biology, Journal Year: 2024, Volume and Issue: 81, P. 102502 - 102502

Published: July 18, 2024

Aberrant Siglec expression in the tumour microenvironment has been implicated malignancies and can impact behaviour patient survival. Further to this, engagement with sialoglycans induces masked antigen recognition promotes immune evasion, highlighting deregulated function. This necessitates elucidation of their profiles progression. MicroRNAs (miRNAs) mediated targeting represents a novel approach further elucidate potential clinical relevance. Although miRNA activity remains limited, we highlight current literature detailing miRNA:Siglec interactions within landscape provide insights for possible diagnostic therapeutic strategies Siglec/sialic acid axis.

Language: Английский

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Potentiating T cell tumor targeting using a combination of TCR with a Siglec-7 based CSR

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: May 13, 2025

Tumors may utilize different strategies to escape T cell immunosurveillance. Besides the overexpression of checkpoint ligands (such as PDL1) or secretion immunosuppressive agents, several studies have shown that cancer aberrant sialylation can, through interaction with selected receptors such those from Siglec family, neutralize NK and function. Herein, we wanted take advantage presence inhibitory sialic acid on tumor surface enhance anti-tumor activity. To this end, devised a novel chimeric receptor consisting extracellular portion Siglec-7 intracellular 41BB, which can convert signals into stimulatory ones when expressed in human T-cells. This co-stimulatory switch (CSR), co-expressed tumor-specific TCR, facilitated higher cytokine activation profiles following co-culture cells. Additionally, cells equipped CSR demonstrated improved function vivo. Given broad expression pattern cells, our data suggest act general adjuvant boost TCR Overall, work provides an approach improve engineered T-cell-based treatment.

Language: Английский

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Innate extracellular mouse Hsp70 inflammatory properties are mediated by the interaction of Siglec-E and LOX-1 receptors

Cell Stress and Chaperones, Journal Year: 2025, Volume and Issue: unknown, P. 100083 - 100083

Published: May 1, 2025

Innate immune responses to cell damage-associated molecular patterns induce a controlled degree of inflammation, ideally avoiding the promotion intense unwanted inflammatory adverse events. When released by damaged cells, Hsp70 can stimulate different that range from activation suppression. The effects are mediated through innate receptors expressed primarily myeloid such as dendritic cells (DCs). regulatory bind extracellular mouse (mHsp70) not fully characterized, and neither their potential interactions with activating receptors. Here, we show mHsp70 interacts receptor complex formed both inhibitory Siglec-E LOX-1 on DCs. We also find this interaction takes place in lipid microdomains within plasma membrane, acts negative regulator LOX-1-mediated upon or oxidized LDL binding. Thus, modulate surface. These findings add another dimension mechanism indicate how self-molecules contribute dampening exacerbated responses.

Language: Английский

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Analysis of SIGLEC12 expression, immunomodulation and prognostic value in renal cancer using multiomic databases

Heliyon, Journal Year: 2024, Volume and Issue: 10(2), P. e24286 - e24286

Published: Jan. 1, 2024

Siglecs belong to a family of immune regulatory receptors predominantly found on hematopoietic cells. They interact with Sia, resulting in the activation or inhibition response. Previous reports have suggested that

Language: Английский

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Sialic acid and Siglec receptors in tumor immunity and immunotherapy

Seminars in Immunology, Journal Year: 2024, Volume and Issue: 74-75, P. 101893 - 101893

Published: July 1, 2024

Language: Английский

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Roles for Siglec-glycan interactions in regulating immune cells

Seminars in Immunology, Journal Year: 2024, Volume and Issue: 77, P. 101925 - 101925

Published: Dec. 19, 2024

Language: Английский

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Tamoxifen Modulates the Immune Landscape of the Tumour Microenvironment: The Paired Siglec-5/14 Checkpoint in Anti-Tumour Immunity in an In Vitro Model of Breast Cancer

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(6), P. 5512 - 5512

Published: March 14, 2023

Since the role of sialome-Siglec axis has been described as a regulatory checkpoint immune homeostasis, promotion stimulatory or inhibitory Siglec-related mechanisms is crucial in cancer progression and therapy. Here, we investigated effect tamoxifen on sialic acid-Siglec interplay its significance conversion breast cancer. To mimic tumour microenvironment, used oestrogen-dependent oestrogen-independent cells/THP-1 monocytes transwell co-cultures exposed to and/or β-estradiol. We found changes cytokine profiles accompanied by phenotype switching, measured expression arginase-1. The immunomodulatory effects THP-1 cells occurred with altered SIGLEC5 SIGLEC14 genes their products, confirmed RT-PCR flow cytometry. Additionally, exposure increased binding Siglec-5 Siglec-14 fusion proteins cells; however, these appeared be unassociated oestrogen dependency. Our results suggest that tamoxifen-induced alterations activity reflect crosstalk between Siglec-expressing tumour's sialome. Given distribution Siglec-5/14, profile activatory Siglecs patients may useful verification therapeutic strategies predicting behaviour patient's overall survival.

Language: Английский

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Innate extracellular Hsp70 inflammatory properties are mediated by the interaction of Siglec-E and LOX-1 receptors

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 4, 2023

Innate immune responses to cell damage-associated molecular patterns induce a controlled degree of inflammation, ideally avoiding the promotion intense unwanted inflammatory adverse events. When released by damaged cells, Hsp70 can stimulate different that range from activation suppression. The effects are mediated through innate receptors expressed primarily myeloid such as dendritic cells (DCs). regulatory bind extracellular mouse (mHsp70) not fully characterized, and neither their potential interactions with activating receptors. Here, we describe mHsp70 interacts receptor complex formed inhibitory Siglec-E LOX-1 on DCs. We also find this interaction takes place within lipid microdomains, acts negative regulator LOX-1-mediated upon or oxidized LDL binding. Thus, HSP70 both modulate surface. These findings add another dimension mechanism how self-molecules contribute dampening exacerbated responses.

Language: Английский

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