Journal of Autoimmunity, Journal Year: 2024, Volume and Issue: 150, P. 103342 - 103342
Published: Dec. 5, 2024
Language: Английский
Advanced Science, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 28, 2025
Abstract Epigenetic modifications serve as crucial molecular switches for pathological fibrosis; howbeit the role of m 1 A in this condition remains enigmatic. Herein, it is found that ALKBH3 exerts a pro‐fibrotic effect skin fibrosis by reshaping N6‐methyladenosine (m 6 A) RNA modification pattern. First, exhibited specific upregulation within hypertrophic scars (HTS), accompanied N1‐methyladenosine hypomethylation. Moreover, multiomics analyses identified METTL3, critical writer enzyme involved modification, downstream candidate target ALKBH3. Therapeutically, ablation inhibited progression HTS both vitro and vivo, while exogenous replenishment METTL3 counteracted antifibrotic effect. Mechanistically, recognizes methylation sites prevents YTHDF2‐dependent mRNA decay transcript. Subsequently, stabilizes collagen type I alpha chain ( COL1A1 ) fibronectin1 FN1 mRNAs, two major components extracellular matrix, therefore eliciting transformation HTS. This observation bridges understanding link between methylation, fundamental modifications, underscoring participation “RNA crosstalk” events.
Language: Английский
Citations
1
Journal of Inflammation Research, Journal Year: 2025, Volume and Issue: Volume 18, P. 283 - 299
Published: Jan. 1, 2025
Lichen sclerosus (LS) is a chronic inflammatory disease affecting skin and mucosal tissues, particularly external genitalia, with risk of cancer. Its etiology unknown, possibly involving immune dysregulation inflammation. Study used DNA methylation (DNAme) single-cell RNA sequencing (scRNA-seq) to compare LS normal skin. A detailed DNAme profile was created, analyzing differentially methylated probes (DMPs) cell type-specific DMPs. EpiSCORE deconvolution infiltration analyses identified altered types in LS. Immunohistochemistry confirmed cellular changes. Enrichment analysis significantly pathways, communication described interactions among patterns generally distinguished from skin, few exceptions. Data showed that T cells increased fibroblasts decreased Immunohistochemical staining the changes cells. DMPs indicated significant impacts on fibroblast-related processes key pathways. The COLLAGEN signal most prominent communication. CD99-CD99 interaction strongest between fibroblasts. Combining scRNA-seq data revealed composition pathways LS, enhancing understanding its pathogenesis highlighting potential therapeutic targets diagnostic markers.
Language: Английский
Citations
0
ACS Nano, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 21, 2025
Cyclophosphamide has a certain therapeutic effect on treating systemic sclerosis (SSc), while difficulties exist in controlling severe systematic side effects and enhancing targeting capacity. Here, inspired from the natural extracellular matrix composition, we propose cyclophosphamide-encapsulated nanogel based dendritic polymers polyamidoamine (PAMAM) for SSc treatment. We combine bovine serum albumin generation 5 (G5) PAMAM dendrimers with polyphenol modification to obtain nanogels featured antioxidant anti-inflammatory effects. The can possess excellent biocompatibility prevent fibroblasts oxidative stress damage TGF-β-mediated activation. Furthermore, bleomycin-induced mouse model, dendrimer encapsulating cyclophosphamide also exhibit ability attenuate fibrosis by modulating immunity, suppressing inflammation, reducing collagen synthesis. These findings underscore value of this polymer treatment chronic SSc, indicating its broader potential clinical applications.
Language: Английский
Citations
0
Nature Reviews Rheumatology, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 14, 2025
Language: Английский
Citations
0
Current Treatment Options in Rheumatology, Journal Year: 2025, Volume and Issue: 11(1)
Published: Feb. 15, 2025
Language: Английский
Citations
0
Clinica Chimica Acta, Journal Year: 2025, Volume and Issue: 570, P. 120209 - 120209
Published: Feb. 21, 2025
Language: Английский
Citations
0
International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3041 - 3041
Published: March 26, 2025
Fibrotic diseases, contributing to a significant portion of global mortality, highlight the need for innovative therapies. This study explores novel approach disrupt expression collagen by using adenine base editing target Col1a1, key gene driving both fibrosis and cancer metastasis. Editing Col1a1 in fibroblasts demonstrated 18% efficiency. An analysis specific clone harboring CCAAT-to-CCGGA mutation promoter revealed reduced production. Notably, when wild-type were cultured on Col1a1-edited matrix, no compensatory upregulation was detected, suggesting lack feedback mechanism fibroblasts. Furthermore, matrix derived from edited did not support growth MCF-7 cells. These findings suggest that holds promise as potential therapeutic strategy fibrotic diseases. Further investigation is warranted fully elucidate implications these cancer.
Language: Английский
Citations
0
Russian Journal of Skin and Venereal Diseases, Journal Year: 2025, Volume and Issue: unknown
Published: March 30, 2025
Folliculitis decalvans (FD)is a rare neutrophilic cicatricial alopecia of the scalp.As it is progressive inflammatory diseasecharacterized by gradual increase in area inflamed follicles with formation areas ofcicatricial alopeciain outcome. The most common clinical manifestations FD are follicular pustules, polytrichia, erosions and hemorrhagic crusts.At later stages dermal fibrosis predominates. exact cause not fully understood, but believed thatStaphylococcus aureusmay play role its development.Current knowledge regarding pathogenesis folliculitis suggests three including hyperactivation innate immunity, bacterial infection as an outcome disease. frequently used treatments topical steroids, antibiotics,and systemic antibacterial drugs, isotretinoin, anti-inflammatory genetically engineered biological laser surgical treatment, all above methods usually result only temporary remission.Phototherapy efficient therapy for variety skin diseases.We hypothesize that wavelengths range 308 nm eradicate bacteria more effectively than antibiotics monotherapy. combination ofultraviolet-B nm, oral antibiotics, combined glucocorticosteroid application bacteriophages, sensitives to identified flora drugs have demonstrated excellent tolerance great outcomesin series cases.
Language: Английский
Citations
0
Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16
Published: April 2, 2025
The intricate interaction between skeletal muscle biomechanics, the tumor microenvironment, and immunotherapy constitutes a pivotal research focus oncology. This work provides comprehensive review of methodologies for evaluating including handheld dynamometry, advanced imaging techniques, electrical impedance myography, elastography, single-fiber experiments to assess quality performance. Furthermore, it elucidates mechanisms, applications, limitations various modalities, immune checkpoint inhibitors, adoptive cell therapy, cancer vaccines, combined chemoimmunotherapy, while examining their effects on function systemic responses. Key findings indicate that although is effective in augmenting antitumor immunity, frequently induces muscle-related adverse such as weakness, fatigue, or damage, primarily mediated by cytokine release activation. underscores significance niches within microenvironment influencing treatment outcomes proposes strategies optimize therapy through personalized regimens combinatorial approaches. highlights need further formation interactions muscle-tumor. Our crucial advancing efficacy immunotherapy, reducing effects, ultimately improving survival rates life patients with cancer.
Language: Английский
Citations
0
Phytomedicine, Journal Year: 2025, Volume and Issue: 142, P. 156787 - 156787
Published: April 25, 2025
Language: Английский
Citations
0