The Easily Overlooked Effect of Global Warming: Diffusion of Heavy Metals

Toxics, Journal Year: 2024, Volume and Issue: 12(6), P. 400 - 400

Published: May 30, 2024

Since industrialization, global temperatures have continued to rise. Human activities resulted in heavy metals being freed from their original, fixed locations. Because of warming, glaciers are melting, carbon dioxide concentrations increasing, weather patterns shifting, and various environmental forces at play, resulting the movement alteration forms. In this general context, impact on ecosystems organisms has changed accordingly. For most ecosystems, levels rise, rise can a negative ecosystem as whole. Numerous studies been conducted analyze combined impacts climate change metals. However, summary current is not perfect. Therefore, review discusses how affect during process multiple perspectives, providing some references for addressing warming

Language: Английский

Remodeling of Embryo Architecture in Response to Vanadium and Increased Temperatures: From Morphometric to Molecular Changes

Journal of Xenobiotics, Journal Year: 2025, Volume and Issue: 15(1), P. 22 - 22

Published: Feb. 1, 2025

The study of ecotoxicity induced by vanadium (V) represents an area increasing interest due to the growing use V in both industrial and pharmaceutical areas. This leads its introduction into water environments, marking a developing problem, especially since rising global temperatures appear intensify toxic properties. Cytotoxicological approaches carried out on whole marine embryos represent valid research tool they grow directly contact with pollutants are equipped highly responsive cells stressors. Here, we discuss detrimental impact Paracentrotus lividus sea urchin resulting from combination higher temperatures, reflecting effects climate variation. results demonstrate remodeling embryonic architecture at morphometric level, revealing developmental delays anomalies. These malformations involve variations total skeletal mass almost absence skeleton, exception small calcareous aggregates. Furthermore, modulation tissue enzymatic activities variation amount three MMP-like gelatinases (MMP-2, -9, -14) were observed. demonstrates that change significantly increases harmful V, emphasizing necessity for comprehensive toxicity assessments environmental evaluations.

Language: Английский

Automated, high-throughput in-situ hybridization of Lytechinus pictus embryos

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Abstract Despite the reach of in situ hybridization (ISH) developmental biology, it has rarely been used at scale. The major limitation throughput assay, which typically relies upon labor intensive manual steps. goal this study was to develop a fully automated chain reaction (HCR) pipeline capable large-scale gene expression pattern profiling, with dramatically reduced cost and effort, sea urchin Lytechinus pictus . Our resulting pipeline, we term high (HT)-HCR, can process 192 probe sets on whole-mount embryos within 32 hours. unique qualities embryo enabled us automate entire HCR assay 96-well plate format, utilize highly miniaturized volumes, general purpose robotic liquid handler, confocal microscopy. From approach produced quality localization data for 101 target genes across three stages L. results reveal previously undescribed physiological genes, as well canonical transcription factors. HT-HCR represents log order increase rate spatial transcriptomic be resolved urchin. This paves way understudied sophisticated perturbation analysis. Summary Statement We developed an high-throughput rapidly map embryos, enabling discovery novel patterns.

Language: Английский