Effects of dietary lipid levels on growth, antioxidant capacity, intestinal and liver structure of juvenile giant salamander (Andrias davidianus) DOI Creative Commons
Xiaolu Yan,

Limei Pan,

Junqi Yu

et al.

Frontiers in Marine Science, Journal Year: 2024, Volume and Issue: 11

Published: Dec. 20, 2024

This research was to assess how varying dietary lipid levels influence the growth, body composition, antioxidant capacity of liver, and structure liver intestine in A. davidianus . The juvenile (initial weight 34.20 ± 0.27g) were given isonitrogenous diets (crude protein 57%) that contained six different (5.02%, 8.96%, 12.90%, 16.21%, 19.46%, 22.80%, respectively) for 105 days. results revealed WGR, SGR, PRR muscle RNA/DNA ratio significantly enhanced by level up beyond which these parameters declined; FCR exhibited an inverse trend( P <0.05). maximum activity intestinal digestive enzymes superoxide dismutase (SOD) observed at 16.21% level, accompanied a significant reduction malondialdehyde (MDA) concentration ( With increase content both whole along with skin collagen content, initially improved before subsequently declining( At height, number, width mucosal folds <0.05), microvilli more orderly arranged. 22.80% cells degradation cell membrane, alongside increased presence autophagosomes; additionally, mitochondria demonstrated swelling rounding. Optimal could enhance feed utilization efficiency promote health In contrast, high has detrimental effect on According ratio, total cholesterol (TCHO) lipase activity, it been determined through quadratic curve model analysis optimal ranges from 13.3% 17.5%.

Language: Английский

Arginine: at the crossroads of nitrogen metabolism DOI Creative Commons
Tak Shun Fung, Keun Woo Ryu, Craig B. Thompson

et al.

The EMBO Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 7, 2025

Abstract L-arginine is the most nitrogen-rich amino acid, acting as a key precursor for synthesis of nitrogen-containing metabolites and an essential intermediate in clearance excess nitrogen. Arginine’s side chain possesses guanidino group which has unique biochemical properties, plays primary role nitrogen excretion (urea), cellular signaling (nitric oxide) energy buffering (phosphocreatine). The post-translational modification protein-incorporated arginine by guanidino-group methylation also contributes to epigenetic gene control. Most human cells do not synthesize sufficient meet demand are dependent on exogenous arginine. Thus, dietary important maintaining health, particularly upon physiologic stress. How adapt changes extracellular availability unclear, mostly because nearly all tissue culture media supplemented with supraphysiologic levels Evidence emerging that arginine-deficiency can influence disease progression. Here, we review new insights into importance metabolite, emphasizing central mitochondria synthesis/catabolism recent discovery act molecule regulating expression organelle dynamics.

Language: Английский

Citations

1
Lower expression of colony-stimulating factor 2, an embryokine, in the endometrial epithelium of old cows DOI

Denis Karani Wanjiru,

Yvan Bienvenu Niyonzima, Hiroya Kadokawa

et al.

Reproduction Fertility and Development, Journal Year: 2025, Volume and Issue: 37(4)

Published: Feb. 13, 2025

Context Infertility increases with age in various animals, including cows, owing to unknown mechanisms. The glandular and luminal epithelia of the bovine uterus synthesise secrete colony-stimulating factor 2 (CSF2), which is a well-studied embryokine. We recently reported possibility fibrosis uteri old cows. However, relationship between CSF2 expression has not yet been clarified. Aims tested hypothesis that endometrial cows have lower compared heifers, myofibroblasts [alpha-smooth muscle actin (αSMA)-positive fibroblasts] increase near epithelium Methods collected caruncle intercaruncle samples from post-pubertal, growing, nulliparous heifers (n = 6; 24.7 ± 1.3 months old) multiparous 128.5 15.4 old). analysed mRNA protein expression, along fluorescent immunohistochemistry for CSF2, anti-collagen type IV, anti-Müllerian hormone receptor, anti-αSMA. Key results Quantitative reverse transcription polymerase chain reaction western blot analysis revealed than young heifers. Fluorescence microscopy using same antibodies anti-αSMA showed increased fibroblasts αSMA signals Conclusion was those Implications Lower may play an important role age-related infertility.

Language: Английский

Citations

0
The role of various collagen types in tumor biology: a review DOI Creative Commons
Yuchuan Zhou, Zhonghui Jiang, Lu Cao

et al.

Frontiers in Oncology, Journal Year: 2025, Volume and Issue: 15

Published: March 5, 2025

Collagen comprises approximately 30% of the body's protein content and is essential for maintaining structural integrity, support, strength skin, muscles, bones, connective tissues. Recent research has further elucidated its role in various aspects tumor biology, including tumorigenesis, invasion, migration, drug resistance, recurrence. Furthermore, collagen involved prognostic assessments, evaluation therapeutic efficacy, immunoregulation, identification potential treatment targets oncology. This review examines a range types, lung, gastric, breast, melanoma, colorectal cancers, among others. Our objective to differentiate these tumors based on specific types present analyze roles development, progression, prognosis, their as targets.

Language: Английский

Citations

0
Beneficial impact of MTGase-modified fish gelatin on collagen supplementation in rats: Insights from serum metabolomics and gut microbiota DOI
Chunyan Peng, Ting Fang,

H.‐T. Lin

et al.

Food Research International, Journal Year: 2025, Volume and Issue: unknown, P. 116295 - 116295

Published: March 1, 2025

Language: Английский

Citations

0
Vitamin C in Cardiovascular Disease: From Molecular Mechanisms to Clinical Evidence and Therapeutic Applications DOI Creative Commons

Yichen Xu,

Huabo Zheng, Ioana Slabu

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(5), P. 506 - 506

Published: April 23, 2025

Vitamin C, also known as ascorbic acid, is an essential nutrient that humans cannot synthesize, making its intake crucial for health. Discovered nearly a century ago, vitamin C widely recognized ability to prevent scurvy and has become one of the most commonly used supplements. Beyond antioxidant activity, pivotal in regulating lipid metabolism, promoting angiogenesis, enhancing collagen synthesis, modulating remodeling, stabilizing extracellular matrix. While preclinical studies have shown promising results, clinical trials yielded inconsistent findings, due suboptimal study design, results misinterpretation, misleading conclusions. This review provides holistic overview existing evidence on pleiotropic role cardiovascular diseases, identifying both strengths limitations current research highlighting gaps understandings C’s underlying mechanisms. By integrating molecular insights with data evaluating disease management prevention, this aims guide future toward personalized, evidence-based therapeutic strategies practice.

Language: Английский

Citations

0
Effects of dietary lipid levels on growth, antioxidant capacity, intestinal and liver structure of juvenile giant salamander (Andrias davidianus) DOI Creative Commons
Xiaolu Yan,

Limei Pan,

Junqi Yu

et al.

Frontiers in Marine Science, Journal Year: 2024, Volume and Issue: 11

Published: Dec. 20, 2024

This research was to assess how varying dietary lipid levels influence the growth, body composition, antioxidant capacity of liver, and structure liver intestine in A. davidianus . The juvenile (initial weight 34.20 ± 0.27g) were given isonitrogenous diets (crude protein 57%) that contained six different (5.02%, 8.96%, 12.90%, 16.21%, 19.46%, 22.80%, respectively) for 105 days. results revealed WGR, SGR, PRR muscle RNA/DNA ratio significantly enhanced by level up beyond which these parameters declined; FCR exhibited an inverse trend( P <0.05). maximum activity intestinal digestive enzymes superoxide dismutase (SOD) observed at 16.21% level, accompanied a significant reduction malondialdehyde (MDA) concentration ( With increase content both whole along with skin collagen content, initially improved before subsequently declining( At height, number, width mucosal folds <0.05), microvilli more orderly arranged. 22.80% cells degradation cell membrane, alongside increased presence autophagosomes; additionally, mitochondria demonstrated swelling rounding. Optimal could enhance feed utilization efficiency promote health In contrast, high has detrimental effect on According ratio, total cholesterol (TCHO) lipase activity, it been determined through quadratic curve model analysis optimal ranges from 13.3% 17.5%.

Language: Английский

Citations

0