Recombinant Type XVII Collagen Inhibits EGFR/MAPK/AP-1 and Activates TGF-β/Smad Signaling to Enhance Collagen Secretion and Reduce Photoaging DOI Creative Commons
Ying He,

Shiyu Yin,

Ru Xu

et al.

Cosmetics, Journal Year: 2025, Volume and Issue: 12(2), P. 59 - 59

Published: March 27, 2025

Studies have consistently shown that long-wave ultraviolet A (UVA) radiation triggers skin photoaging, which is evident as reduced elasticity, a loss of firmness, and signs aging. There an urgent need to investigate photoaging mechanisms devise protective strategies against UVA. The present study aimed explore the effects recombinant type XVII collagen on UVA-induced aging uncover its molecular mechanisms, thereby laying solid theoretical foundation for precise treatments prevention. We therefore modeled damage in HaCaT cells evaluated collagen-related protein gene expression levels via western blot analysis real-time quantitative polymerase chain reaction analysis. Immunofluorescent staining was also used assess secretion basement membrane expression. Recombinant significantly boosted IV collagen, laminin alpha 5, integrin β1 production, thus counteracting decline. revealed matrix metalloproteinase (MMP) downregulation tissue inhibitor (TIMP) upregulation. Modulating transforming growth factor (TGF)-β/Smad epidermal receptor (EGFR)/mitogen-activated kinase (MAPK)/activator protein-1 (AP-1) pathways suppressed photoaging. Together, our findings suggest ameliorates by reversing MMP TIMP expression, preventing degradation enhancing secretion. These results offer basis potent anti-photoaging products, paving way innovative solutions

Language: Английский

Recombinant Type XVII Collagen Inhibits EGFR/MAPK/AP-1 and Activates TGF-β/Smad Signaling to Enhance Collagen Secretion and Reduce Photoaging DOI Creative Commons
Ying He,

Shiyu Yin,

Ru Xu

et al.

Cosmetics, Journal Year: 2025, Volume and Issue: 12(2), P. 59 - 59

Published: March 27, 2025

Studies have consistently shown that long-wave ultraviolet A (UVA) radiation triggers skin photoaging, which is evident as reduced elasticity, a loss of firmness, and signs aging. There an urgent need to investigate photoaging mechanisms devise protective strategies against UVA. The present study aimed explore the effects recombinant type XVII collagen on UVA-induced aging uncover its molecular mechanisms, thereby laying solid theoretical foundation for precise treatments prevention. We therefore modeled damage in HaCaT cells evaluated collagen-related protein gene expression levels via western blot analysis real-time quantitative polymerase chain reaction analysis. Immunofluorescent staining was also used assess secretion basement membrane expression. Recombinant significantly boosted IV collagen, laminin alpha 5, integrin β1 production, thus counteracting decline. revealed matrix metalloproteinase (MMP) downregulation tissue inhibitor (TIMP) upregulation. Modulating transforming growth factor (TGF)-β/Smad epidermal receptor (EGFR)/mitogen-activated kinase (MAPK)/activator protein-1 (AP-1) pathways suppressed photoaging. Together, our findings suggest ameliorates by reversing MMP TIMP expression, preventing degradation enhancing secretion. These results offer basis potent anti-photoaging products, paving way innovative solutions

Language: Английский

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