ACS Nano,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 9, 2025
The
assembly
of
peptides
is
generally
mediated
by
liquid–liquid
phase
separation,
which
enables
control
over
kinetics,
final
structure,
and
functions
peptide-based
supramolecular
materials.
Modulating
separation
can
alter
the
kinetics
changing
solvents
or
introducing
external
fields.
Herein,
we
demonstrate
that
be
effectively
catalyzed
complex
coacervates.
negatively
charged
sodium
alginate
(SA)
form
coacervates
with
positively
KLVFFAE
(Aβ16–22,
abbreviated
as
KE)
peptide,
thereby
lowering
nucleation
barrier
promoting
peptide.
As
binding
affinity
SA-KE
dosage
SA
decrease,
system
shifts
from
a
relatively
inefficient
template-induced
to
highly
efficient
catalytic
before
ultimately
reverting
slow
spontaneous
assembly.
Therefore,
both
well
stoichiometry
do
not
follow
intuitive
rule
"more
better",
but
rather
there
exists
an
optimal
value
maximizes
rate
Accounts of Chemical Research,
Journal Year:
2024,
Volume and Issue:
57(3), P. 289 - 301
Published: Jan. 17, 2024
ConspectusBiomolecular
self-assembly
is
a
ubiquitous
occurrence
in
nature
that
gives
rise
to
sophisticated
superstructures
enable
the
implementation
of
complex
biological
functions.
It
encompasses
both
ordered
structures,
such
as
DNA
double
helix,
and
disordered
nucleolus
other
nonmembranous
organelles.
In
contrast
these
highly
organized
which
exhibit
specific
patterns
or
symmetry,
structures
are
characterized
by
their
flexible
randomized
molecular
organization,
provides
versatility,
dynamicity,
adaptability
systems
contributes
complexity
functionality
living
organisms.
However,
usually
exist
thermodynamically
metastable
state.
This
means
unstable
difficult
observe
due
short
existence
time.
Achieving
through
precise
control
assembly
process
ensuring
stability
integrity
pose
significant
challenges.
Currently,
ongoing
research
efforts
focused
on
proteins
with
intrinsically
regions
(IDRs).
structural
instability
present
prohibitive
difficulties
elucidating
multiscale
process.
Therefore,
simple
peptides,
segment
proteins,
hold
great
promise
constructing
for
related
research.
Since
our
finding
droplet-like
occur
transiently
during
peptide
(PSA),
centered
around
dynamic
evolution
supramolecular
systems,
particularly
modulation
variety
assembled
ranging
from
disordered.In
this
Account,
we
narrate
recent
endeavors
formed
PSA,
spanning
structures.
We
delve
into
mechanisms
regulation,
shedding
light
how
peptide-based
can
be
controlled
more
precisely.
Moreover,
emphasize
functional
applications
arise
To
begin,
conduct
comprehensive
overview
various
types
emerge
showcasing
diverse
applications.
Following,
elaborate
discovery
development
PSA.
A
mechanistic
study
multistep
processes
mediated
liquid–liquid
phase
separation
(LLPS)
critically
emphasized.
Ordered
different
morphologies
functions
obtained
subtly
controlling
adjusting
liquid
droplets.
particular,
have
recently
developed
solid
glasses
long-range
disorder,
including
noncovalent
biomolecular
glass
based
amino
acid
derivatives,
well
high-entropy
cyclic
peptides.
demonstrates
potential
using
biologically
derived
molecules
create
green
sustainable
glassy
materials.
Journal of the American Chemical Society,
Journal Year:
2024,
Volume and Issue:
146(18), P. 12577 - 12586
Published: April 29, 2024
Dynamic
supramolecular
assemblies,
driven
by
noncovalent
interactions,
pervade
the
biological
realm.
In
synthetic
domain,
their
counterparts,
polymers,
endowed
with
remarkable
self-repair
and
adaptive
traits,
are
often
realized
through
bioinspired
designs.
Recently,
controlled
polymerization
strategies
have
emerged,
drawing
inspiration
from
protein
self-assembly.
A
burgeoning
area
of
research
involves
mimicking
liquid–liquid
phase
separation
(LLPS)
observed
in
proteins
to
create
coacervate
droplets
recognizing
significance
cellular
organization
diverse
functions.
Herein,
we
introduce
a
novel
perspective
on
coacervates,
extending
beyond
established
role
biology
as
dynamic,
membraneless
phases
enable
structural
control
polymers.
Drawing
parallels
cooperative
growth
amyloid
fibrils
LLPS,
present
metastable
dormant
monomer
for
polymerization.
This
is
achieved
via
π-conjugated
design
that
combines
characteristics
both
coacervation
its
terminal
ionic
groups
one-dimensional
core.
leads
unique
temporal
resulting
phase,
which
subsequently
undergoes
nucleation
within
droplets.
In-depth
spectroscopic
microscopic
characterization
provides
insights
into
evolution
disordered
ordered
phases.
Furthermore,
modulate
kinetics
liquid-to-solid
transformation
achieve
precise
over
invoke
seeding
droplets,
showcasing
living
characteristics.
Our
work
thus
opens
up
new
avenues
exciting
field
polymerization,
offering
general
principles
synthesis
precision
self-assembled
structures
confined
environments.
Advanced Functional Materials,
Journal Year:
2024,
Volume and Issue:
34(25)
Published: Feb. 21, 2024
Abstract
Peptide‐based
supramolecular
therapeutics
(PST)
refer
to
those
theranostic
nanomedicines
based
on
peptide
or
derivatives
self‐assembly
that
are
constructed
in
vitro
vivo.
Benefiting
from
the
inherent
advantages
of
peptides,
such
as
good
biocompatibility,
high
bioactivity,
and
flexible
tunability,
reported
PST
have
shown
excellent
application
prospects
combating
many
major
diseases
including
cancer,
cardiovascular
disease,
infectious
autoimmune
metabolic
so
on.
Through
thoughtful
design,
can
be
fabricated
various
forms,
self‐assembled
precursor
molecules,
functional
nano‐assemblies,
macroscopic
large‐scale
hydrogels.
The
involved
peptides
play
different
roles,
serving
drug
carriers
for
effective
delivery,
acting
scaffold
materials
cell
culture,
functioning
bioactive
sequences
treating
diseases.
In
this
review,
a
comprehensive
overview
traditional
strategies
is
provided
build
emphasize
their
therapeutic
applications
fighting
pose
threat
human
life
health.
Moreover,
main
challenges
systems
application,
clinical
translation
also
briefly
discussed.
It
hoped
review
arouse
much
more
attention
paid
promote
its
practical
biomedical
field.
Materials Horizons,
Journal Year:
2024,
Volume and Issue:
11(16), P. 3946 - 3960
Published: Jan. 1, 2024
Zwitterionic
hydrogels
are
electrically
neutral
materials
with
both
cationic
and
anionic
groups
that
impart
excellent
anti-fouling
properties
ion
channel
orientations.
However,
pure
zwitterionic
generally
exhibit
low
strength
toughness.
In
this
study,
it
has
been
discovered
polymerizable
monomers
in
aqueous
solution
a
unique
liquid-liquid
phase
separation
phenomenon
at
high
monomer
concentration
of
≥50
wt%,
resulting
commercial
compressive
(6.5
MPa)
toughness
(2.12
kJ
m
