ACS Nano,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 9, 2025
The
assembly
of
peptides
is
generally
mediated
by
liquid–liquid
phase
separation,
which
enables
control
over
kinetics,
final
structure,
and
functions
peptide-based
supramolecular
materials.
Modulating
separation
can
alter
the
kinetics
changing
solvents
or
introducing
external
fields.
Herein,
we
demonstrate
that
be
effectively
catalyzed
complex
coacervates.
negatively
charged
sodium
alginate
(SA)
form
coacervates
with
positively
KLVFFAE
(Aβ16–22,
abbreviated
as
KE)
peptide,
thereby
lowering
nucleation
barrier
promoting
peptide.
As
binding
affinity
SA-KE
dosage
SA
decrease,
system
shifts
from
a
relatively
inefficient
template-induced
to
highly
efficient
catalytic
before
ultimately
reverting
slow
spontaneous
assembly.
Therefore,
both
well
stoichiometry
do
not
follow
intuitive
rule
"more
better",
but
rather
there
exists
an
optimal
value
maximizes
rate
Biomacromolecules,
Journal Year:
2024,
Volume and Issue:
25(9), P. 5468 - 5488
Published: Aug. 23, 2024
Biomolecular
condensates
are
dynamic
liquid
droplets
through
intracellular
liquid-liquid
phase
separation
that
function
as
membraneless
organelles,
which
highly
involved
in
various
complex
cellular
processes
and
functions.
Artificial
analogs
formed
via
similar
pathways
can
be
integrated
with
biological
complexity
advanced
functions
have
received
tremendous
research
interest
the
field
of
synthetic
biology.
The
coacervate
droplet-based
compartments
partition
concentrate
a
wide
range
solutes,
regarded
attractive
candidates
for
mimicking
phase-separation
behaviors
biophysical
features
biomolecular
condensates.
use
peptide-based
materials
phase-separating
components
has
advantages
such
diversity
amino
acid
residues
customized
sequence
design,
allows
programming
their
physicochemical
properties
resulting
compartments.
In
this
Perspective,
we
highlight
recent
advancements
design
construction
biomimicry
from
peptides
relevant
to
protein,
specific
reference
molecular
self-assembly
separation,
biorelated
applications,
envisage
emerging
biomedical
delivery
vehicles.
Angewandte Chemie International Edition,
Journal Year:
2023,
Volume and Issue:
62(52)
Published: Nov. 8, 2023
Supramolecular
peptide
assemblies
have
been
widely
used
for
the
development
of
biomedical,
catalytical,
and
optical
materials
with
chiral
nanostructures
in
view
intrinsic
chirality
peptides.
However,
assembly
pathway
transformation
behavior
various
peptides
remain
largely
elusive
especially
transient
under
out-of-equilibrium
conditions.
Herein,
N-fluorenylmethoxycarbonyl-protected
phenylalanine-tyrosine
dipeptide
(Fmoc-FY)
was
as
a
platform,
which
showed
that
proceeds
multistep
evolution.
The
original
spheres
caused
by
liquid-liquid
phase
separation
(LLPS)
can
nucleate
elongate
into
formation
right-handed
helices
were
metastable
easily
converted
microribbons.
Interestingly,
bipyridine
derivative
be
introduced
to
effectively
control
induce
thermodynamically
stable
or
left-handed
at
different
stoichiometric
ratios.
In
addition,
also
regulated
ultrasound
enzyme
catalysis.
This
minimalistic
system
not
only
broadens
nucleation-elongation
mechanisms
protein
aggregates
but
promotes
controllable
design
biomaterials.
Journal of the American Chemical Society,
Journal Year:
2024,
Volume and Issue:
146(43), P. 29759 - 29766
Published: Oct. 15, 2024
The
supramolecular
assembly
of
proteins
into
irreversible
fibrils
is
often
associated
with
diseases
in
which
aberrant
phase
transitions
occur.
Due
to
the
complexity
biological
systems
and
their
surrounding
environments,
mechanism
underlying
separation-mediated
poorly
understood,
making
reversal
so-called
fibrillization
a
significant
challenge.
Therefore,
it
crucial
develop
simple
model
that
provide
insights
mechanistic
process
monomers
phase-separated
droplets
ordered
assemblies.
Such
models
can
help
investigating
strategies
either
reverse
or
modulate
these
states.
Herein,
we
present
synthetic
system
composed
three
components,
including
benzene-1,3,5-tricarboxamide-based
monomer,
surfactant,
water,
mimic
condensate
pathway
observed
systems.
This
highly
dynamic
undergo
"micelle-droplet-fiber"
transition
over
time
space
concentration
gradient
field,
regulated
by
competitive
interactions.
Importantly,
manipulating
interactions
through
guest
molecules,
temperature
changes,
cosolvents
fibers
disordered
liquid
micellar
state.
Our
provides
new
critical
balance
between
various
among
components
determine
reversibility
process.
Extending
this
"competitive
interaction"
approach
from
complex
macromolecules,
e.g.,
proteins,
could
open
avenues
for
biomedical
applications,
such
as
condensate-modifying
therapeutics.
ACS Nano,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 9, 2025
The
assembly
of
peptides
is
generally
mediated
by
liquid–liquid
phase
separation,
which
enables
control
over
kinetics,
final
structure,
and
functions
peptide-based
supramolecular
materials.
Modulating
separation
can
alter
the
kinetics
changing
solvents
or
introducing
external
fields.
Herein,
we
demonstrate
that
be
effectively
catalyzed
complex
coacervates.
negatively
charged
sodium
alginate
(SA)
form
coacervates
with
positively
KLVFFAE
(Aβ16–22,
abbreviated
as
KE)
peptide,
thereby
lowering
nucleation
barrier
promoting
peptide.
As
binding
affinity
SA-KE
dosage
SA
decrease,
system
shifts
from
a
relatively
inefficient
template-induced
to
highly
efficient
catalytic
before
ultimately
reverting
slow
spontaneous
assembly.
Therefore,
both
well
stoichiometry
do
not
follow
intuitive
rule
"more
better",
but
rather
there
exists
an
optimal
value
maximizes
rate
