Epigenetic responses in Borrelia-infected Ixodes scapularis ticks: Over-expression of euchromatic histone lysine methyltransferase 2 and no change in DNA methylation DOI Creative Commons

Grace Hadley MacIntosh,

Alexandra C. Nuyens,

Jane Vickery

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 27, 2024

Abstract Borrelia burgdorferi , a tick-vectored spirochete bacteria best known for causing Lyme disease, has been found to induce physiological and behavioural changes in its tick vector that can increase fitness ability transmit the bacteria. The mechanism by which this bacterium modulates these remains unknown. Epigenetics plays central role transducing external internal microbiome environmental influences organism, so we investigated DNA methylation expression of key histone modification enzyme -infected uninfected Ixodes scapularis ticks. pericentromeric tandem repeats family, Repeats (ISR) were assessed methylated-DNA immunoprecipitation (MeDIP) followed qPCR ISR regions. sequences was found. different had levels methylation, however, not significantly affected presence or absence B. . epigenetic regulator euchromatic lysine methyltransferase 2 ( EHMT2 ) is recognized as having modulating organismal stress response infections. To assess transcription ticks, real-time reverse transcriptase PCR performed. Uninfected ticks over 800X lower than infected This study among first identify gene may be involved producing differences depending on infection status lays groundwork future studies I. well other pathogens transmit.

Language: Английский

Epigenomic Changes in Ostrinia Moths Under Elevated Pupal and Adult Temperature DOI Open Access
Brittany A Velikaneye, Genevieve M. Kozak

Molecular Ecology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

ABSTRACT Epigenetic changes in the methylation of DNA may occur response to environmental stressors, including warming climates. also play an important role regulating gene expression during both male and female reproduction many insect species. However, it is currently unknown how shifts when individuals are reproducing under warmer temperatures. We exposed European corn borer moths ( Ostrinia nubilalis ) heat pupal adult life stages then investigated across genome using enzymatic methyl‐seq (EM‐seq). compared patterns reproductive males females (28°C) those that experienced ambient temperature (23°C). found exposure led a small but significant increase percentage methylated CpG sites throughout sexes. rates were higher differential following localised unique regions each sex. In males, shifted within genes belonging pathways Hippo signalling, ubiquitin‐mediated proteolysis, damage repair spermatogenesis. females, occurred related histone modification oogenesis. Our results suggest respond moderate Lepidoptera provide insight into epigenetic responses heatwaves, suggesting novel be involved responding stress metamorphosis reproduction.

Language: Английский

Citations

0
Female reproductive molecules—Still much to learn DOI
Sonja Grath, Mareike Koppik, Claudia Fricke

et al.

Advances in insect physiology, Journal Year: 2024, Volume and Issue: unknown, P. 49 - 103

Published: Jan. 1, 2024

Language: Английский

Citations

0
Hormonal regulation and disruption in invertebrates – an historical perspective and recent findings DOI
Anapaula Sommer Vinagre, Angela B. Lange, João C. R. Cardoso

et al.

Molecular and Cellular Endocrinology, Journal Year: 2024, Volume and Issue: 593, P. 112335 - 112335

Published: July 30, 2024

Language: Английский

Citations

0
Epigenetic responses in Borrelia-infected Ixodes scapularis ticks: Over-expression of euchromatic histone lysine methyltransferase 2 and no change in DNA methylation DOI Creative Commons

Grace Hadley MacIntosh,

Alexandra C. Nuyens,

Jane Vickery

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 27, 2024

Abstract Borrelia burgdorferi , a tick-vectored spirochete bacteria best known for causing Lyme disease, has been found to induce physiological and behavioural changes in its tick vector that can increase fitness ability transmit the bacteria. The mechanism by which this bacterium modulates these remains unknown. Epigenetics plays central role transducing external internal microbiome environmental influences organism, so we investigated DNA methylation expression of key histone modification enzyme -infected uninfected Ixodes scapularis ticks. pericentromeric tandem repeats family, Repeats (ISR) were assessed methylated-DNA immunoprecipitation (MeDIP) followed qPCR ISR regions. sequences was found. different had levels methylation, however, not significantly affected presence or absence B. . epigenetic regulator euchromatic lysine methyltransferase 2 ( EHMT2 ) is recognized as having modulating organismal stress response infections. To assess transcription ticks, real-time reverse transcriptase PCR performed. Uninfected ticks over 800X lower than infected This study among first identify gene may be involved producing differences depending on infection status lays groundwork future studies I. well other pathogens transmit.

Language: Английский

Citations

0