14‐3‐3 proteins: Regulators of cardiac excitation–contraction coupling and stress responses

The Journal of Physiology, Journal Year: 2025, Volume and Issue: unknown

Published: May 11, 2025

Abstract 14‐3‐3 proteins are highly conserved that regulate numerous cellular processes mostly through phosphorylation‐dependent protein–protein interactions. In the heart play critical roles in cardiac conduction pathways, excitation–contraction (EC) coupling, development and stress responses. This review summarizes current understanding of regulation function, with particular emphasis on its role ion channel β‐adrenergic signalling. We discuss how act three main mechanisms – masking, clamping, scaffolding to target proteins, including Cx43, Ca V 1.2, Na 1.5, various potassium channels. The seven mammalian isoforms display distinct but overlapping functions, tissue‐specific expression patterns isoform‐specific phosphorylation dimerization. Recent work has revealed 14‐3‐3's importance responses, where it generally serves a cardioprotective role. However some pathological contexts such as ischaemia–reperfusion injury, can be detrimental. highlight emerging themes biology, prolonging Understanding complex targets presents both opportunities challenges for therapeutic development. image

Language: Английский

Regulation of H9C2 cell hypertrophy by 14-3-3η via inhibiting glycolysis

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(7), P. e0307696 - e0307696

Published: July 22, 2024

It has been reported that Ywhah (14-3-3η) reduces glycolysis. However, it remains unclear about the downstream mechanism by which glycolysis is regulated 14-3-3η in cardiac hypertrophy. As an important regulator, Yes-associated protein (YAP) interacts with to participate initiation and progression of various diseases vivo. In this study, model H9C2 cardiomyocyte hypertrophy was established triiodothyronine (T3) or rotenone stimulation probe into action 14-3-3η. Interestingly, overexpression attenuated T3 induced decreased cardiomyocytes, whereas knockdown had opposite effect. Mechanistically, can reduce expression level YAP bind its nuclear translocation. addition, changing may affect lactate dehydrogenase A (LDHA), a glycolysis-related protein. Meanwhile, LDHA also possible target for mediate based on changes pyruvate, substrate LDHA. Collectively, suppress via decreasing nucleus translocation glycolysis, indicates could be promising inhibiting

Language: Английский