Discovery and Verification of Sjögren’s Syndrome Protein Biomarkers in Tears by Targeted LC-MRM DOI

Maggy Lépine,

Marie‐Claude Robert, Lekha Sleno

et al.

Journal of Proteome Research, Journal Year: 2024, Volume and Issue: 23(6), P. 2219 - 2229

Published: April 29, 2024

Sjögren's syndrome (SS) is an autoimmune rheumatic disorder characterized by exocrine gland dysfunction, mainly from the lacrimal and salivary glands. The disease causes severe aqueous dry eye (DED) associated with high rates of complications, including corneal ulceration, scaring, perforation. Systemic complications may occur as well a higher risk developing lymphoma. Diagnosis SS-DED often delayed difficult to establish. With aim discovering biomarkers help discriminate patients, combination untargeted targeted LC-MS/MS analyses were performed on tear samples collected Schirmer strips subjected tryptic digestion. Following analysis three cohorts development two LC-sMRM methods for verification putative found in first cohort samples, 64 proteins could be linked syndrome, hopes helping confirm diagnoses potentially stratifying severity these patients. Proteins that increased showed activation immune system alterations homeostasis. Several proteases protease inhibitors significantly changing SS-DED, consistent decrease specific known secreted gland.

Language: Английский

Secretome analysis using Affinity Proteomics and Immunoassays: a focus on Tumor Biology DOI Creative Commons
Vanessa M. Beutgen, V. A. Shinkevich,

Johanna Pörschke

et al.

Molecular & Cellular Proteomics, Journal Year: 2024, Volume and Issue: 23(9), P. 100830 - 100830

Published: Aug. 14, 2024

The study of the cellular secretome using proteomic techniques continues to capture attention research community across a broad range topics in biomedical research. Due their untargeted nature, independence from model system used, historically superior depth analysis, as well comparative affordability, mass spectrometry-based approaches traditionally dominate such analyses. More recently, however, affinity-based assays have massively gained analytical depth, which together with high sensitivity, dynamic coverage throughput capabilities render them exquisitely suited analysis. In this review, we revisit challenges implied by secretomics and provide an overview platforms currently available for analyses, tumor example basic translational

Language: Английский

Citations

6
Decoding the MMP14 Integrin Link: Key Player in the Secretome Landscape DOI Creative Commons
Stephan Niland, Johannes A. Eble

Matrix Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Rapid progress has been made in the exciting field of secretome research health and disease. The tumor secretome, which is a significant proportion proteome, secreted into extracellular space to promote intercellular communication thus progression. Among many molecules integrins matrix metalloproteinase 14 (MMP14) stand out as interplay adhesion proteolysis drives invasion. Integrins serve mechanosensors that mediate contact cells with scaffold are significantly involved precise positioning activity control membrane-bound collagenase MMP14. As proteinase, MMP14 influences modifies itself. While MT-MMPs membrane bound, but can be released therefore border crossers between cell surface not constitutively cell-bound, its binding other receptors stringently regulated process. To understand mutual interactions detail, we first summarize structure function how it at enzymatic cellular level. In particular, include proteolytic cleavage themselves by We then review biochemical, biological physiological effects on composition associated functions when either bound membrane, or located microvesicles, proteolytically shed non-membrane-bound ectodomain. Novel methods proteomics, including analysis extravesicular vesicles, new for quantification will provide diagnostic tools. modification especially MMP14, may bring an additional aspect studies have impact diagnosis most likely also therapy cancer patients.

Language: Английский

Citations

0
A Primer on Proteomic Characterization of Intercellular Communication in a Virus Microenvironment DOI Creative Commons
James Kostas,

Colter S Brainard,

Ileana M. Cristea

et al.

Molecular & Cellular Proteomics, Journal Year: 2025, Volume and Issue: unknown, P. 100913 - 100913

Published: Jan. 1, 2025

Language: Английский

Citations

0
Alternative splicing of CHI3L1 regulates protein secretion through conformational changes DOI

Haesoo Jung,

Yong‐Eun Kim,

Eun‐Mi Kim

et al.

Genes & Genomics, Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Language: Английский

Citations

0
Method standardization of secretome production, collection, and characterization: New insights and challenges DOI
Naveen P. Singh, Yahya E. Choonara, Pradeep Kumar

et al.

Regenerative Therapy, Journal Year: 2025, Volume and Issue: 29, P. 466 - 473

Published: April 24, 2025

Language: Английский

Citations

0
Small HSPs at the crossroad between protein aggregation, autophagy and unconventional secretion: clinical implications and potential therapeutic opportunities in the context of neurodegenerative diseases DOI Creative Commons
Raffaella Bonavita,

Fulvia Vitale,

Luigi Vittorio Verdicchio

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: May 2, 2025

Neurodegenerative diseases (NDs) such as Alzheimer’s, Parkinson’s and Huntington’s well ataxias fronto-temporal disorders are all characterized by the progressive accumulation of protein aggregates (amyloids) into inclusions bodies. In addition, recent experimental evidence is challenging conventional view disease revealing ability some these disease-relevant proteins to be transferred between cells means extracellular vesicles (EVs), allowing mutant seed oligomers involving both wild type forms protein. Abnormal secretion levels EVs closely related pathogenesis neurodegenerative contribute progression. Numerous studies have proposed therapeutic targets or biomarkers for diseases. this review, we summarize discuss role small heat shock (sHSPs) autophagy in cellular quality control turn-over major aggregation-prone associated disorders. We also highlight advanced research progress on mechanisms regulating unconventional secretion, secretory biogenesis their contribution pathological processes underlining Finally, outline latest roles potential diagnostic significance treatment clinically relevant conditions.

Language: Английский

Citations

0
Comprehensive secretome profiling and CRISPR screen identifies SFRP1 as a key inhibitor of epidermal progenitor proliferation DOI Creative Commons
Binbin Cheng, Shaohong Peng, Yunlong Jia

et al.

Cell Death and Disease, Journal Year: 2025, Volume and Issue: 16(1)

Published: May 3, 2025

Abstract Secreted proteins are crucial for the structure and functions of human epidermis, but full repertoire keratinocyte secretome has not been experimentally defined. In this study, we performed mass spectrometry on conditioned media from primary keratinocytes, identifying 406 with diverse roles in adhesion, migration, proliferation, proteolysis, signal transduction, innate immunity. To leverage new dataset, developed a novel colony formation assay-based CRISPR screen to investigate uncharacterized secreted epidermal stem cells. The identified six candidate that promoted proliferation progenitors two inhibited it. frizzled-related protein-1 (SFRP1) was most potent inhibitor. We discovered SFRP1 restrained clonogenic by inhibiting Wnt signaling as well blocking ectopic expression leukemia inhibitory factor (LIF). Collectively, our study expands knowledge secretome, establishes assess function non-cell autonomous factors, highlights SFRP1’s role regulating balance.

Language: Английский

Citations

0
Discovery and Verification of Sjögren’s Syndrome Protein Biomarkers in Tears by Targeted LC-MRM DOI

Maggy Lépine,

Marie‐Claude Robert, Lekha Sleno

et al.

Journal of Proteome Research, Journal Year: 2024, Volume and Issue: 23(6), P. 2219 - 2229

Published: April 29, 2024

Sjögren's syndrome (SS) is an autoimmune rheumatic disorder characterized by exocrine gland dysfunction, mainly from the lacrimal and salivary glands. The disease causes severe aqueous dry eye (DED) associated with high rates of complications, including corneal ulceration, scaring, perforation. Systemic complications may occur as well a higher risk developing lymphoma. Diagnosis SS-DED often delayed difficult to establish. With aim discovering biomarkers help discriminate patients, combination untargeted targeted LC-MS/MS analyses were performed on tear samples collected Schirmer strips subjected tryptic digestion. Following analysis three cohorts development two LC-sMRM methods for verification putative found in first cohort samples, 64 proteins could be linked syndrome, hopes helping confirm diagnoses potentially stratifying severity these patients. Proteins that increased showed activation immune system alterations homeostasis. Several proteases protease inhibitors significantly changing SS-DED, consistent decrease specific known secreted gland.

Language: Английский

Citations

2