Transcriptional insights on the incomplete cytoplasmic maturation and developmental potential of oocytes cultured without granulosa cells in mice DOI Creative Commons
Yibo Wang, Yichuan Zhang, Tianjie Li

et al.

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: March 18, 2025

Oocyte maturation is crucial for female fertility and embryonic development, encompassing nuclear cytoplasmic maturation. Supportive cells of follicles, such as granulosa cells, are essential oocyte growth Oocytes can achieve without during in vitro (IVM). However, there still a higher chance incomplete these oocytes with mature nuclei compared cultured cells. have lower fertilization rates developmental potential than ones, although underlying mechanisms poorly understood. Identifying key genes signaling pathways associated help further elucidate the maturing process understand impact immature on throwing insights into strategy to improve success rate assisted reproductive technologies. Our study investigated murine IVM yielded vivo (IVO). Even though could they showed featuring levels reactive oxygen species, mitochondrial density, proportions abnormal distributions cortical granules. Of note, cytoplasm had distinct transcriptional profiles. In oocytes, we observed deficient mRNA restoration regulatory cellular division, potentially affecting development. Differentially expressed (DEGs) between were identified be highly different pre-implantation stages, MII oocyte, 8-cell stage, ICM stage. Identified DEGs enriched energy metabolic pathways. These observations indicated that impeded development might result gene expressions We show important both oocytes. Abnormal expression may defects

Language: Английский

Transcriptional insights on the incomplete cytoplasmic maturation and developmental potential of oocytes cultured without granulosa cells in mice DOI Creative Commons
Yibo Wang, Yichuan Zhang, Tianjie Li

et al.

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: March 18, 2025

Oocyte maturation is crucial for female fertility and embryonic development, encompassing nuclear cytoplasmic maturation. Supportive cells of follicles, such as granulosa cells, are essential oocyte growth Oocytes can achieve without during in vitro (IVM). However, there still a higher chance incomplete these oocytes with mature nuclei compared cultured cells. have lower fertilization rates developmental potential than ones, although underlying mechanisms poorly understood. Identifying key genes signaling pathways associated help further elucidate the maturing process understand impact immature on throwing insights into strategy to improve success rate assisted reproductive technologies. Our study investigated murine IVM yielded vivo (IVO). Even though could they showed featuring levels reactive oxygen species, mitochondrial density, proportions abnormal distributions cortical granules. Of note, cytoplasm had distinct transcriptional profiles. In oocytes, we observed deficient mRNA restoration regulatory cellular division, potentially affecting development. Differentially expressed (DEGs) between were identified be highly different pre-implantation stages, MII oocyte, 8-cell stage, ICM stage. Identified DEGs enriched energy metabolic pathways. These observations indicated that impeded development might result gene expressions We show important both oocytes. Abnormal expression may defects

Language: Английский

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