Comparison of antimicrobial resistant Enterobacterales isolates from the dairy production environment in low and high zinc containing regions DOI Creative Commons
Elisa Anedda, Elena A. Alexa,

M.L. Farrell

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 953, P. 175905 - 175905

Published: Aug. 30, 2024

Heavy metals occur naturally in the environment, and their concentration varies soil across different regions. However, presence of heavy may influence antimicrobial resistance (AMR) bacterial populations. Therefore, objective this study was to investigate characterise profiles Enterobacterales bovine milk filters from high low zinc-containing regions Ireland. In total, 50 samples 29 were collected two geographic locations with varying zinc concentrations. Samples cultured for enumeration detection Enterobacterales. Specifically, extended-spectrum beta-lactamase-producing Enterobacterales, carbapenem-resistant ciprofloxacin-resistant isolated using selective media. Species identification performed MALDI-TOF. The phenotypic selected determined by disk diffusion testing, following EUCAST CLSI criteria; while, genotypic same isolates whole genome sequencing (WGS). metal concentrations also measured all samples. A total 40 resistant identified (n = 31) 9). predominant species detected region Escherichia coli both sample types (soil n 10, 2), while Serratia fonticola 8) E. 4). Ten multidrug resistant, showing antimicrobials tested, except carbapenems. WGS findings confirmed results. Moreover, resistance-associated genes encoding efflux pumps identified. current revealed distinct regions, highlighted benefit utilising AMR surveillance dairy production.

Language: Английский

Recent Advances in Fluorescent Nanoprobes for Food Safety Detection DOI Creative Commons
Huanxiang Yuan, Yutong Li,

Jiaqi Lv

et al.

Molecules, Journal Year: 2023, Volume and Issue: 28(14), P. 5604 - 5604

Published: July 24, 2023

Fluorescent nanoprobes show similar fluorescence properties to traditional organic dyes, but the addition of nanotechnology accurately controls size, shape, chemical composition, and surface chemistry with unique characteristics properties, such as bright luminescence, high photostability, strong biocompatibility. For example, modifying aptamers or antibodies on a fluorescent nanoprobe provides selectivity specificity for different objects be tested. Fluorescence intensity, life, other parameters targets can changed by sensing mechanisms based structural optical nanoprobes. What’s more, detection is cost-saving, simple, offers great advantages in rapid food detection. Sensing were introduced this paper, focusing application progress pesticide residues, veterinary drug heavy metals, microbes, mycotoxins, substances safety recent years. A brief outlook future development was provided well.

Language: Английский

Citations

9

Comparison of antimicrobial resistant Enterobacterales isolates from the dairy production environment in low and high zinc containing regions DOI Creative Commons
Elisa Anedda, Elena A. Alexa,

M.L. Farrell

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 953, P. 175905 - 175905

Published: Aug. 30, 2024

Heavy metals occur naturally in the environment, and their concentration varies soil across different regions. However, presence of heavy may influence antimicrobial resistance (AMR) bacterial populations. Therefore, objective this study was to investigate characterise profiles Enterobacterales bovine milk filters from high low zinc-containing regions Ireland. In total, 50 samples 29 were collected two geographic locations with varying zinc concentrations. Samples cultured for enumeration detection Enterobacterales. Specifically, extended-spectrum beta-lactamase-producing Enterobacterales, carbapenem-resistant ciprofloxacin-resistant isolated using selective media. Species identification performed MALDI-TOF. The phenotypic selected determined by disk diffusion testing, following EUCAST CLSI criteria; while, genotypic same isolates whole genome sequencing (WGS). metal concentrations also measured all samples. A total 40 resistant identified (n = 31) 9). predominant species detected region Escherichia coli both sample types (soil n 10, 2), while Serratia fonticola 8) E. 4). Ten multidrug resistant, showing antimicrobials tested, except carbapenems. WGS findings confirmed results. Moreover, resistance-associated genes encoding efflux pumps identified. current revealed distinct regions, highlighted benefit utilising AMR surveillance dairy production.

Language: Английский

Citations

1