Understanding the impact of methoxy groups of undescribed atropisomer biflavonoids of methanol leaf extract of Ochna rhizomatosa against chloroquine-resistant Plasmodium falciparum strain NF54: Insight from experimental, DFT and molecular docking studies

Journal of Molecular Structure, Journal Year: 2024, Volume and Issue: unknown, P. 141201 - 141201

Published: Dec. 1, 2024

Language: Английский

Designing Microfluidic PCR Chip Device Using CFD Software for the Detection of Malaria

Published: Aug. 10, 2023

Polymerase chain reaction (PCR) technique is one of the molecular methods in amplifying DNA for detection malaria. However, limitations PCR especially when used routine clinical practice can hamper its sensitivity and specificity. With that, this study focuses on designing a microfluidic device that will mimic function conventional genus-specific based 18S rRNA gene to detect malaria parasites (Plasmodium falciparum) at low grade parasitemia. The design was drawn simulated using ANSYS 14.5 Computational Fluid Dynamics (CFD). simulation shows adding loops increases relative deviation but minimal extent as compared having only straight path design, which indicates looping acceptable minimize chip length. Also, increasing cross-sectional area fluid decreases efficiency thus, with relatively smaller favored. And lastly, among three materials utilized, made polypropylene most efficient 0.94 polycarbonate polydimethylsiloxane have deviations 2.78 1.92, respectively.

Language: Английский

Designing Microfluidic PCR Chip Device Using CFD Software for the Detection of Malaria

Computation, Journal Year: 2023, Volume and Issue: 11(10), P. 190 - 190

Published: Sept. 30, 2023

Polymerase chain reaction (PCR) technique is one of the molecular methods in amplifying DNA for detection malaria. However, collection and transportation samples processing dissemination results via conventional PCR, especially when used routine clinical practice, can hamper technique’s sensitivity specificity. The rampancy such disease Philippines aggravated by limited supply medical machinery poor economic state country; thus, need to innovate a device early malaria necessary. With that, this study focuses on designing microfluidic that will mimic function genus-specific PCR based 18S rRNA gene detect parasites (Plasmodium falciparum) at low-grade parasitemia. design was intended be portable, accessible, economical, which none from past literature has dealt with specifically detection. This silico first country specially crafted reasons. proposed developed simulated using ANSYS software Computational Fluid Dynamics (CFD) analyses. simulation shows adding loops increases its relative deviation but minimally compared having only straight path design. indicates looping acceptable minimize chip length. It also found increasing cross-sectional area fluid decreases efficiency Lastly, among three materials utilized, made polypropylene most efficient, 0.94 polycarbonate polydimethylsiloxane, have deviations 2.78 1.92, respectively. Future researchers may mesh 44-cycle due limitations study, other materials, as biocomposites, assessed broaden application

Language: Английский