SUN1 inhibits osteogenesis and promotes adipogenesis of human adipose‐derived stem cells by regulating α‐tubulin and CD36 expression DOI Creative Commons
Tingyu Fan, Jinhui Zhu, Wenqing Liu

et al.

Journal of Cellular and Molecular Medicine, Journal Year: 2024, Volume and Issue: 28(19)

Published: Oct. 1, 2024

Sad and UNC84 domain 1 (SUN1) is a kind of nuclear envelope protein with established involvement in cellular processes, including motility meiosis. SUN1 plays an intriguing role human adipose-derived stem cells (hASCs) differentiation; however, this remains largely undefined. This study was undertaken to investigate the hASCs differentiation, as well its underlying mechanisms. Employing siRNAs, we selectively downregulated CD36 expression. Microtubules were depolymerized using nocodazole, PPARγ activated rosiglitazone. Western blotting performed quantify SUN1, PPARγ, α-tubulin, CD36, OPN, adiponectin expression levels. Alkaline phosphatase Oil red O staining used assess osteogenesis adipogenesis, respectively. Downregulated increased decreased adipogenesis hASCs, concomitant upregulated α-tubulin expression, alongside reduced localization PPARγ. Microtubule depolymerization Rescue experiments indicated that microtubule counteracted SUN1-induced phenotypic changes. demonstrates influences differentiation towards osteogenic adipogenic lineages, indicating essential cell fate.

Language: Английский

Arterial arcades and collaterals regress under hemodynamics-based diameter adaptation: A computational and mathematical analysis DOI Creative Commons
Vivi Rottschäfer,

Willem G.N. Kuppers,

Jiao Chen

et al.

Journal of Theoretical Biology, Journal Year: 2025, Volume and Issue: unknown, P. 112111 - 112111

Published: April 1, 2025

Language: Английский

Citations

0
Islet dimension and its impact on transplant outcome: A systematic review DOI

Sipra Rout,

P Mishra, Appakalai N. Balamurugan

et al.

World Journal of Transplantation, Journal Year: 2025, Volume and Issue: 15(3)

Published: April 18, 2025

BACKGROUND Not all islet transplants desirably achieve insulin independence. This can be attributed to the microarchitecture and function of islets influenced by their dimensions. Large enhance secretion through paracrine effects but are more susceptible hypoxic injury post-transplant, while small offer better viability In vivo studies suggest large essential for maintaining euglycemia, though smaller typically preferred in transplantation outcomes. AIM To document impact dimension on clinical preclinical transplant outcomes optimize procedures. METHODS PubMed, Scopus EMBASE platforms were searched relevant literature up 9 April 2024. Articles reported either glucose-stimulated insulin-secreting (GSIS) capacity, engraftment, or independence based included. The risk bias was measured using Appraisal Tool Cross-Sectional Studies. Extracted data analyzed via a narrative synthesis. RESULTS Nineteen included review. A total sixteen GSIS, which nine documented increased islet, where majority per equivalent (IEQ). Seven GSIS large-sized that measure cell islet. All articles compared poor engraftment islets. CONCLUSION Small with diameter < 125 µm have desired due survival following isolation. Large-sized receive blood supply directly from arterioles meet higher metabolic demands. undergoes central necrosis soon after isolation (devascularization); failing maintain glucose stimuli leads decline overall Improved preservation isolation, enhances yield (IEQ), thereby reducing likelihood failed potentially improves outcome.

Language: Английский

Citations

0
The efficiency of stem cell differentiation into functional beta cells for treating insulin-requiring diabetes: Recent advances and current challenges DOI
Yunfei Luo, Peng Yu, Jianping Liu

et al.

Endocrine, Journal Year: 2024, Volume and Issue: 86(1), P. 1 - 14

Published: May 10, 2024

Language: Английский

Citations

3
Ameliorating and refining islet organoids to illuminate treatment and pathogenesis of diabetes mellitus DOI Creative Commons
Yushan Li,

Meiqi Xu,

Jiali Chen

et al.

Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)

Published: June 27, 2024

Abstract Diabetes mellitus, a significant global public health challenge, severely impacts human worldwide. The organoid, an innovative in vitro three-dimensional (3D) culture model, closely mimics tissues or organs vivo. Insulin-secreting islet derived from stem cells induced with 3D structures, has emerged as potential alternative for transplantation and possible disease model that mirrors the body’s vivo environment, eliminating species difference. This technology gained considerable attention its diabetes treatment. Despite advances, process of cell differentiation into organoid cultivation demonstrates deficiencies, prompting ongoing efforts to develop more efficient protocols biomimetic materials. At present, constructed exhibit limitations their composition, structure, functionality when compared natural islets. Consequently, further research is imperative achieve multi-tissue system composition improved insulin secretion while addressing transplantation-related safety concerns, such tumorigenicity, immune rejection, infection, thrombosis. review delves methodologies strategies constructing application treatment, pivotal scientific challenges within research, offering fresh perspectives deeper understanding pathogenesis development therapeutic interventions.

Language: Английский

Citations

0
Arterial arcades and collaterals regress under hemodynamics-based diameter adaptation: a computational and mathematical analysis DOI Creative Commons

Vivi Rottschsfer,

Willem G.N. Kuppers,

Jiao Chen

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 23, 2024

Segments in the arterial network have a >1000-fold span of radii. This is believed to result from adaptation each segment wall shear stress (WSS), with outward respectively inward remodeling if WSS higher or lower than some reference value. While this seems straightforward mechanism for tree design, not but contains numerous arcades, collaterals and other looping structures. In theoretical study, we analyzed stability structures networks under control. Simulation models were based on very simple topologies as well published human coronary mouse cerebral networks. Adaptation was implemented rate change structural radius that proportional deviation its WSS. A more generalized model large range local hemodynamic stimuli, including velocity, flow power dissipation. For over 12,000 tested parameter sets, simulations invariably predicted loss loops due regression one segments. small networks, case both model, initial conditions parameters. Loss loopiness also by included direction-dependent rates, heterogeneous rates among adapting segments, dynamic conditions. found artery subjected mathematical analysis proved direct consequence Kirchhoff's circuit law, which loop leads positive eigenvalue Jacobian matrix partial derivatives therefore unstable equilibria presence loops. an inherent property adapt hemodynamics. Additional mechanisms are needed explain their presence, communication between connected

Language: Английский

Citations

0
SUN1 inhibits osteogenesis and promotes adipogenesis of human adipose‐derived stem cells by regulating α‐tubulin and CD36 expression DOI Creative Commons
Tingyu Fan, Jinhui Zhu, Wenqing Liu

et al.

Journal of Cellular and Molecular Medicine, Journal Year: 2024, Volume and Issue: 28(19)

Published: Oct. 1, 2024

Sad and UNC84 domain 1 (SUN1) is a kind of nuclear envelope protein with established involvement in cellular processes, including motility meiosis. SUN1 plays an intriguing role human adipose-derived stem cells (hASCs) differentiation; however, this remains largely undefined. This study was undertaken to investigate the hASCs differentiation, as well its underlying mechanisms. Employing siRNAs, we selectively downregulated CD36 expression. Microtubules were depolymerized using nocodazole, PPARγ activated rosiglitazone. Western blotting performed quantify SUN1, PPARγ, α-tubulin, CD36, OPN, adiponectin expression levels. Alkaline phosphatase Oil red O staining used assess osteogenesis adipogenesis, respectively. Downregulated increased decreased adipogenesis hASCs, concomitant upregulated α-tubulin expression, alongside reduced localization PPARγ. Microtubule depolymerization Rescue experiments indicated that microtubule counteracted SUN1-induced phenotypic changes. demonstrates influences differentiation towards osteogenic adipogenic lineages, indicating essential cell fate.

Language: Английский

Citations

0