Plasma biomarkers in patients with age-related sarcopenia: a proteomic exploration and experimental validation DOI Creative Commons
Qinqing Lin,

Kangyong Li,

Liwei Li

et al.

Aging Clinical and Experimental Research, Journal Year: 2024, Volume and Issue: 37(1)

Published: Dec. 27, 2024

Abstract Background Various biomarkers associated with sarcopenia have been identified. However, there is a scarcity of studies exploring and validating in individuals age-related sarcopenia. Aims This study aimed to investigate the proteome identify potential for Methods Proteomic analysis experimental validation were conducted using plasma from hospitalized older adults. Sarcopenia diagnosis was based on Asian Working Group 2019 criteria. Data-independent acquisition-based proteomics performed 60 participants, 30 diagnosed without Differentially expressed proteins (DEPs) selected evaluated by Receiver Operating Characteristic (ROC) analysis. Biomarker candidates further quantitatively validated enzyme-linked immunosorbent assay (ELISA) utilizing 6 participants Results A total 39 DEPs identified 12 ROC 8 included ELISA their predictive performance. Paraoxonase-3 (PON3) consistently showed down-regulation sarcopenic group across both methodologies. Insulin-like growth factor-binding protein-2 (IGFBP2) inconsistency group, up-regulation observed proteomic but ELISA. Discussion Decline PON3 may result an overload oxidative stress skeletal muscles contribute Protein modifications IGFBP2 might exhibit during pathogenesis. Conclusions Plasma are implicated highlighted as biomarker patients Further imperative gain in-depth understanding IGFBP2.

Language: Английский

Intermittent fasting attenuates CNS inflammaging - rebalancing the transposonome DOI Creative Commons
Mitchell J Cummins, Ethan T Cresswell, Doug W. Smith

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

Abstract A hallmark of CNS aging is sterile, chronic, low-grade neuroinflammation. Understanding how the develops chronic inflammation necessary to achieve extended healthspan. Characterisation neuroinflammatory molecular triggers remains limited. Interventions that reduce neuroinflammation and extend health lifespan could be useful in this regard. One such intervention intermittent fasting (IF), but IF impacts insufficiently understood. To address this, we performed deep RNA-sequencing on young, middle-aged, old, mouse regions. Additionally, sequenced spinal cord animals subject adult lifelong IF. We found most differentially expressed genes (DEGs) at middle age were region specific (~ 50–84%), whilst effect weakened 18–72%) old age, suggesting emergence a more general global profile. DEGs from all regions enriched for inflammatory immune ontologies. Surprisingly, SC was aging- neuroinflammation-impacted both ages, with by far highest number DEGs, largest net increase expression transposable elements (TEs), greatest enrichment immune-related ontologies, generally larger increases gene expression. Overall, normal upregulation sensors non-self, DNA/RNA, activation inflammasomes, cGAS-STING1 interferon response genes, across CNS. Whilst still developed an profile SC, average lower ~ 50% compared age-matched controls. IF-specific apparent, also acts separate, potentially targetable, pathways those impacted aging. Expression disease associated microglia, phagocytic exhaustion, STING1, inflammasome decreased Significantly, TE reversed decrease. In summary, find hotspot, attenuates neuroinflammaging rebalancing transposonome.

Language: Английский

Citations

0
Plasma biomarkers in patients with age-related sarcopenia: a proteomic exploration and experimental validation DOI Creative Commons
Qinqing Lin,

Kangyong Li,

Liwei Li

et al.

Aging Clinical and Experimental Research, Journal Year: 2024, Volume and Issue: 37(1)

Published: Dec. 27, 2024

Abstract Background Various biomarkers associated with sarcopenia have been identified. However, there is a scarcity of studies exploring and validating in individuals age-related sarcopenia. Aims This study aimed to investigate the proteome identify potential for Methods Proteomic analysis experimental validation were conducted using plasma from hospitalized older adults. Sarcopenia diagnosis was based on Asian Working Group 2019 criteria. Data-independent acquisition-based proteomics performed 60 participants, 30 diagnosed without Differentially expressed proteins (DEPs) selected evaluated by Receiver Operating Characteristic (ROC) analysis. Biomarker candidates further quantitatively validated enzyme-linked immunosorbent assay (ELISA) utilizing 6 participants Results A total 39 DEPs identified 12 ROC 8 included ELISA their predictive performance. Paraoxonase-3 (PON3) consistently showed down-regulation sarcopenic group across both methodologies. Insulin-like growth factor-binding protein-2 (IGFBP2) inconsistency group, up-regulation observed proteomic but ELISA. Discussion Decline PON3 may result an overload oxidative stress skeletal muscles contribute Protein modifications IGFBP2 might exhibit during pathogenesis. Conclusions Plasma are implicated highlighted as biomarker patients Further imperative gain in-depth understanding IGFBP2.

Language: Английский

Citations

0