Vitamin D alleviates oxidative stress in varicose veins: a pilot study in obese and non-obese patients DOI Creative Commons

Sonia Rațiu,

Mihaela Maris,

Adina V. Furdui-Lința

et al.

Molecular and Cellular Biochemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

Abstract Chronic venous disease and varicose veins of the lower extremities represent a widespread pathology, particularly in individuals with obesity. A high prevalence vein has been observed obese patients association plasma levels vitamin D. The present pilot study aimed to investigate acute effects 1,25-dihydroxyvitamin D 3 [1,25(OH) 2 ], biologically active form D, on oxidative stress obtained from both non-obese undergoing cryostripping surgery for ablation. Varicose samples treated or not 1,25(OH) (100 nM, 12-h incubation) were analysed reactive oxygen species (ROS) generation using ferrous xylenol orange oxidation (FOX) assay immunofluorescence technique. Additionally, gene expression endothelial nitric oxide synthase (eNOS), neuronal (nNOS), inducible (iNOS) was assessed via qPCR. We report significant reduction circulating 25-hydroxyvitamin [25(OH)D ] as compared patients. Ex vivo incubation resulted in: (i) ROS level, (ii) upregulation eNOS nNOS expression, (iii) downregulation iNOS groups Vitamin did exhibit scavenger effect, antioxidant effect is presumably mediated its receptor whose presence confirmed samples. In conclusion, exerts protective which may be beneficial administration prior surgical intervention. Large clinical trials are required assess optimal dosage time/duration chronic indication.

Language: Английский

Vitamin D alleviates oxidative stress in varicose veins: a pilot study in obese and non-obese patients DOI Creative Commons

Sonia Rațiu,

Mihaela Maris,

Adina V. Furdui-Lința

et al.

Molecular and Cellular Biochemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

Abstract Chronic venous disease and varicose veins of the lower extremities represent a widespread pathology, particularly in individuals with obesity. A high prevalence vein has been observed obese patients association plasma levels vitamin D. The present pilot study aimed to investigate acute effects 1,25-dihydroxyvitamin D 3 [1,25(OH) 2 ], biologically active form D, on oxidative stress obtained from both non-obese undergoing cryostripping surgery for ablation. Varicose samples treated or not 1,25(OH) (100 nM, 12-h incubation) were analysed reactive oxygen species (ROS) generation using ferrous xylenol orange oxidation (FOX) assay immunofluorescence technique. Additionally, gene expression endothelial nitric oxide synthase (eNOS), neuronal (nNOS), inducible (iNOS) was assessed via qPCR. We report significant reduction circulating 25-hydroxyvitamin [25(OH)D ] as compared patients. Ex vivo incubation resulted in: (i) ROS level, (ii) upregulation eNOS nNOS expression, (iii) downregulation iNOS groups Vitamin did exhibit scavenger effect, antioxidant effect is presumably mediated its receptor whose presence confirmed samples. In conclusion, exerts protective which may be beneficial administration prior surgical intervention. Large clinical trials are required assess optimal dosage time/duration chronic indication.

Language: Английский

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