Crosstalk between Pseudomonas aeruginosa antibiotic resistance and virulence mediated by phenylethylamine

Microbial Biotechnology, Journal Year: 2023, Volume and Issue: 16(7), P. 1492 - 1504

Published: March 28, 2023

Abstract Multidrug efflux pumps are among the main Pseudomonas aeruginosa antibiotic‐resistance determinants. Besides, also involved in other relevant activities of bacterial physiology, including quorum sensing‐mediated regulation virulence. Nevertheless, despite relevance their interconnection with metabolism remains obscure. The effect several metabolites on expression P. pumps, and virulence antibiotic resistance this bacterium, was studied. Phenylethylamine found to be both inducer substrate MexCD‐OprJ, an pump extrusion precursors quorum‐sensing signals. did not increase resistance; however, production toxin pyocyanin, tissue‐damaging protease LasB swarming motility were reduced presence metabolite. This decrease potential mediated by a reduction lasI pqsABCDE expression, which encode proteins that synthesise signalling molecules two regulatory pathways. work sheds light between determinants, metabolism, points phenylethylamine as anti‐virulence metabolite considered study therapies against infections.

Language: Английский

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What are the missing pieces needed to stop antibiotic resistance?

Microbial Biotechnology, Journal Year: 2023, Volume and Issue: 16(10), P. 1900 - 1923

Published: July 7, 2023

Abstract As recognized by several international agencies, antibiotic resistance is nowadays one of the most relevant problems for human health. While this problem was alleviated with introduction new antibiotics into market in golden age antimicrobial discovery, few are pipeline. Under these circumstances, a deep understanding on mechanisms emergence, evolution and transmission resistance, as well consequences bacterial physiology acquiring needed to implement novel strategies, beyond development or restriction use current ones, more efficiently treat infections. There still aspects field that not fully understood. In article, we make non‐exhaustive critical review some them consider special relevance, aim presenting snapshot studies need be done tackle resistance.

Language: Английский

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Prioritization of Critical Factors for Surveillance of the Dissemination of Antibiotic Resistance in Pseudomonas aeruginosa: A Systematic Review

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(20), P. 15209 - 15209

Published: Oct. 15, 2023

Pseudomonas aeruginosa is the primary opportunistic human pathogen responsible for a range of acute and chronic infections; it poses significant threat to immunocompromised patients leading cause morbidity mortality nosocomial infections. Its high resistance diverse array antimicrobial agents presents an urgent health concern. Among mechanisms contributing in P. aeruginosa, horizontal acquisition antibiotic genes (ARGs) via mobile genetic elements (MGEs) has gained recognition as substantial concern clinical settings, thus indicating that comprehensive understanding ARG dissemination within species strongly required surveillance. Here, two approaches, including systematic literature analysis genome database survey, were employed gain insights into dissemination. The enabled scrutinizing all available sequence information various attributes isolates, providing extensive species. By integrating both with focus on ARGs linked or correlated MGEs, important types (STs) carrying ARGs, MGEs identified critical factors requiring strict Although isolates play role dissemination, importance animal environmental also been suggested. In this study, 25 45 STs, associated involved species, are suggested factors. Surveillance management these prioritized across One Health sectors essential mitigate emergence multidrug-resistant (MDR) extensively resistant (XDR) settings.

Language: Английский

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Pathogen profile of Baiyangdian Lake sediments using metagenomic analysis and their correlation with environmental factors

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 913, P. 169628 - 169628

Published: Dec. 28, 2023

Language: Английский

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The MexJK Multidrug Efflux Pump Is Not Involved in Acquired or Intrinsic Antibiotic Resistance in Pseudomonas aeruginosa, but Modulates the Bacterial Quorum Sensing Response

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(14), P. 7492 - 7492

Published: July 6, 2022

Multidrug efflux pumps are critical elements in both intrinsic and acquired antibiotic resistance of bacterial populations. Consequently, most studies regarding these protein machineries focus on this specific phenotype. Nevertheless, different works show that participate other aspects physiology too. Herein, we study the Pseudomonas aeruginosa multidrug pump MexJK. Previous studies, using model strains lacking MexAB-OprM MexCD-OprJ pumps, support MexJK can extrude erythromycin, tetracycline, triclosan. However, results here reported indicate potential increased extrusion, a mutant overexpressing mexJK, does not alter antibiotics susceptibility wild-type genetic background where all remain functional. clear impact quorum sensing (QS) response, mainly Pqs-dependent QS regulation network expression Pqs-regulated virulence factors, was observed linked to mexJK overexpression. The production siderophore pyoverdine strongly depended level expression, suggesting might P. pyoverdine-dependent iron homeostasis. All all, presented current article functions as MexJK, go beyond modulate relevant physiology.

Language: Английский

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