The Role of Methanogenic Archaea in Inflammatory Bowel Disease—A Review

Journal of Personalized Medicine, Journal Year: 2024, Volume and Issue: 14(2), P. 196 - 196

Published: Feb. 10, 2024

Methanogenic archaea are a part of the commensal gut microbiota responsible for hydrogen sink and efficient production short-chain fatty acids. Dysbiosis methanogens is suspected to play role in pathogenesis variety diseases, including inflammatory bowel disease (IBD). Unlike bacteria, diversity seems be higher IBD patients compared healthy subjects, whereas prevalence abundance declines IBD, especially ulcerative colitis. To date, studies focusing on pediatric very limited; nevertheless, preliminary results provide some evidence that may influenced by chronic process IBD. In this review, we demonstrated development methanogenic community both adults children.

Language: Английский

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Ether lipids from archaeas in nano-drug delivery and vaccination

International Journal of Pharmaceutics, Journal Year: 2023, Volume and Issue: 634, P. 122632 - 122632

Published: Jan. 21, 2023

Language: Английский

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Human associated Archaea: a neglected microbiome worth investigating

World Journal of Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 40(2)

Published: Jan. 4, 2024

Language: Английский

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Expanding the cultivable human archaeome:Methanobrevibacter intestinisp. nov. and strainMethanobrevibacter smithii “GRAZ-2”from human feces

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 16, 2024

Abstract Two mesophilic, hydrogenotrophic methanogens, WWM1085 and M. smithii GRAZ-2 were isolated from human fecal samples. was an individual in the USA, represents a novel species with genus Methanobrevibacter . (= DSM 116045) retrieved samples of European, healthy female strain within this genus. Both representatives form non-flagellated, short rods variable morphologies capacity to filaments. isolates showed typical fluorescence F 420 methane production. Compared GRAZ-2, did not accumulate formate when grown on H 2 CO The optimal growth conditions at 37°C, pH 7. Full genome sequencing revealed genomic difference type PS (type strain; 861), 93.55% ANI major differences sequence its mcrA gene (3.3% nucleotide sequence). Differences 16S rRNA very minor thus distinction based might be possible. identified as (ANI 99.04 % PS). Due phenotypic, metabolic features, we propose intestini sp. nov. (DSM 116060T = CECT 30992).

Language: Английский

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Targeted isolation of Methanobrevibacter strains from fecal samples expands the cultivated human archaeome

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Aug. 31, 2024

Abstract Archaea are vital components of the human microbiome, yet their study within gastrointestinal tract (GIT) is limited by scarcity cultured representatives. Our presents a method for targeted enrichment and isolation methanogenic archaea from fecal samples. The procedure combines methane breath testing, in silico metabolic modeling, media optimization, FACS, dilution series, genomic sequencing through Nanopore technology. Additional analyzes include co-cultured bacteriome, comparative genomics archaeal genomes, functional comparisons, structure-based protein function prediction unknown differential traits. Successful establishment stable cultures 14 out 16 samples yielded nine previously uncultivated strains, eight which absent recent archaeome genome catalog. Comparative assessments Methanobrevibacter smithii Candidatus intestini strains individual donors revealed features potentially associated with diseases. work broadens available representatives GIT studies, offers insights into genomes’ adaptability critical microbiome contexts.

Language: Английский

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Human Milk Archaea Associated with Neonatal Gut Colonization and Its Co-Occurrence with Bacteria

Microorganisms, Journal Year: 2025, Volume and Issue: 13(1), P. 85 - 85

Published: Jan. 4, 2025

Archaea have been identified as early colonizers of the human intestine, appearing from first days life. It is hypothesized that origin many these archaea through vertical transmission during breastfeeding. In this study, we aimed to characterize archaeal composition in samples mother-neonate pairs observe potential transmission. We performed a cross-sectional study characterizing diversity 40 colostrum-neonatal stool by next-generation sequencing V5-V6 16S rDNA libraries. Intra- and inter-sample analyses were carried out describe Archaeal each sample type. Human colostrum neonatal stools presented similar core microbiota, mainly composed methanogens Methanoculleus Methanosarcina. Beta metabolic prediction results suggest homogeneity between types. Further, co-occurrence network analysis showed associations Bacteria, which might be relevant for organisms' presence milk ecosystems. According relative abundance proportions, beta diversity, analyses, similarities found imply there Nonetheless, differential abundances types other sources colonizing gut.

Language: Английский

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Reevaluation of the gastrointestinal methanogenic archaeome in multiple sclerosis and its association with treatment

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

ABSTRACT The role of the gut archaeal microbiome (archaeome) in health and disease remains poorly understood. Methanogenic archaea have been linked to multiple sclerosis (MS), but prior studies were limited by small cohorts inconsistent methodologies. To address this, we re-evaluated association between methanogenic MS using metagenomic data from International Multiple Sclerosis Microbiome Study. We analyzed profiles 115 patients healthy household controls across Buenos Aires (27.8%), Edinburgh (33.9%), New York (10.4%), San Francisco (27.8%). Metagenomic sequences taxonomically classified kraken2/bracken a curated profiling database detect archaea, specifically Methanobrevibacter species. Most female (80/115), aged 25–72 years (median: 44.5), 70% undergoing treatment, including dimethyl fumarate ( n = 21), fingolimod 20), glatiramer acetate 14), interferon 18), natalizumab 6), or ocrelizumab/rituximab 1). found no significant differences overall archaeome controls. However, treated exhibited higher abundances smithii M. sp900766745 compared untreated patients. Notably, abundance correlated with lower severity scores Our results suggest that methanogens are not directly associated onset progression may reflect during treatment. These findings highlight potential roles for modulating treatment outcomes, warranting further investigation into their relevance function management. IMPORTANCE (MS) is chronic neuroinflammatory affecting central nervous system, approximately 2.8 million people diagnosed worldwide, mainly young adults 20–30 years. While recent focused on bacterial changes microbiome, has less explored. Previous research suggested link status, these remained inconclusive. study addresses this gap investigating composition examining how it response By focusing methanogens, aim uncover novel insights MS, potentially revealing new biomarkers therapeutic targets. This crucial enhancing our understanding microbiome’s impact improving patient

Language: Английский

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Culturable Human Microorganisms and the Impact of Transportation Conditions on Cultivability

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 549 - 549

Published: Feb. 28, 2025

The composition of the human microbiome is a critical health indicator, and culture-independent methodologies have substantially advanced our understanding human-associated microorganisms. However, precise identification characterization microbial strains require culture-based techniques. Recently, resurgence culturomics, combined with high-throughput sequencing technology, has reduced high labor demand pure culture methods, facilitating more efficient comprehensive acquisition culturable strains. This study employed an integrated approach combining culturomic to identify microorganisms on scalp in saliva feces. Several Staphylococcus were identified from scalp, whereas anaerobic dominant fecal samples. Additionally, highlighted beneficial effects transportation conditions (liquid nitrogen treatment, dry ice transport, dimethyl sulfoxide [DMSO] buffer) preserving A robust methodology was developed for large-scale optimized transport that enhance potential isolating greater diversity

Language: Английский

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A Spotlight on Archaea in Humans, Livestock and Poultry: A Review

Veterinary Medicine and Science, Journal Year: 2025, Volume and Issue: 11(2)

Published: March 1, 2025

ABSTRACT The microbiota includes prokaryotes (archaea and bacteria) eukaryotes. Archaea are single‐celled essential part of gut microbiome. Researches on archaea in ruminants humans more than mono‐gastric. low abundance the depends method used (metagenomics or meta‐transcriptomic) age people poultry. lack complete recognition is due to their small number identifying them (16S rRNA gene primers). uses include analytical kit, reduce oil pollution, archaeosomes drugs production, vaccines agents, lipid carriers pharmaceutical industry molybdenum extraction nuclear industry. nutritional functions methanogenic including feed utilization (ruminants) efficiency, hydrogen reducing (human), fat deposition enhancement energy harvesting mice, CAZymes genes, cecal fermentation, syntrophic potential, carotenoid source improved transit time appetite SCFAs production. acting as antibiotics (produce archaeocins, sulfolobicins halocin KPS1) probiotics (archaeobiotics) can TMAU (trimethylaminuria) disease, cardiovascular diseases (CVDs), atherosclerosis, brain abscess, cancer, colorectal inflammatory bowel disease (IBD), constipation, obesity, food allergies, asthma anti‐inflammation which be prevented by using archaea, other homeostasis, heat shock protein (HSP) production aging.

Language: Английский

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Biohythane production via anaerobic digestion process: fundamentals, scale-up challenges, and techno-economic and environmental aspects

Environmental Science and Pollution Research, Journal Year: 2024, Volume and Issue: 31(38), P. 49935 - 49984

Published: Aug. 1, 2024

Abstract Biohythane, a balanced mixture comprising bioH 2 (biohydrogen) and bioCH 4 (biomethane) produced through anaerobic digestion, is gaining recognition as promising energy source for the future. This article provides comprehensive overview of biohythane production, covering production mechanisms, microbial diversity, process parameters. It also explores different feedstock options, bioreactor designs, scalability challenges, along with techno-economic environmental assessments. Additionally, discusses integration into waste management systems examines future prospects enhancing efficiency applicability. review serves valuable resource researchers, engineers, policymakers interested in advancing sustainable renewable solution.

Language: Английский

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