Shifts in fungal community diversity and potential function under natural forest succession and planted forest restoration in the Kunyu Mountains, East China DOI Creative Commons
Zhu Ping,

Xinyu Hu,

Qiang Zou

et al.

Ecology and Evolution, Journal Year: 2024, Volume and Issue: 14(8)

Published: Aug. 1, 2024

Abstract Soil fungi participate in various ecosystem processes and are important factors driving the restoration of degraded forests. However, little is known about changes fungal diversity potential functions under development different vegetation types during natural (secondary forest succession) anthropogenic (reforestation) restoration. In this study, we selected typical succession sequences (including Pinus densiflora Siebold & Zucc., pine‐broadleaf mixed P. Quercus acutissima Carruth., Q. ), as well secondary deciduous broadleaved forests planted Robinia pseudoacacia on Kunyu Mountain for analysis. We used ITS rRNA gene sequencing to characterize communities FUNGuild database predict functional groups. The results showed that affected β‐diversity, but not α‐diversity. There was a significant increase Basidiomycota decrease Ascomycota later successional stage, accompanied by an groups ectomycorrhizal (ECM). Conversely, exhibited decreased α‐diversity altered community compositions, characterized fewer more Mucoromycota. Planted led relative abundances ECM animal pathogens. TK content major factor explaining distinction among three stages, whereas pH, AP, NH 4 + were variations between Changes significantly soil reforestation, providing key insights management temperate

Language: Английский

A Simplified and Integrated View of Disease Control in Varietal Mixtures Using the Phytobiome Framework DOI Creative Commons
Laura Mathieu, Elsa Ballini, Jean‐Benoit Morel

et al.

Plant Cell & Environment, Journal Year: 2025, Volume and Issue: unknown

Published: April 8, 2025

ABSTRACT Increasing intraspecific diversity within crop systems is a promising strategy to manage aerial diseases, particularly those caused by fungal pathogens. This review examines how cultivar mixtures reduce disease incidence and severity using the phytobiome framework, identifying three major types of processes: (1) physical ones, which alter dynamics through dilution effects, barrier microclimate modifications; (2) processes that are mediated microbial interactions, influence via induced resistance indirect plant–plant interactions microbiome; (3) involving direct where danger signaling from healthy neighbors modulate plant physiology immunity resource management molecular cues. provides comprehensive understanding enhance emphasizes likely stronger contributors than so far considered. It highlights need for further research into roles microbiomes optimize mixtures' performance.

Language: Английский

Citations

0
Carposphere microbiota alters grape volatiles and shapes the wine grape typicality DOI
Menglong Liu, Xuenan Yao, Haiqi Wang

et al.

New Phytologist, Journal Year: 2025, Volume and Issue: unknown

Published: April 18, 2025

Summary While specific environments are known to shape plant metabolomes and the makeup of their associated microbiome, it is as yet unclear whether carposphere microbiota contribute characteristics grape fruit flavor a particular wine region. Here, microbiomes berry transcriptomes three cultivars growing at six geographic sites were analyzed. The composition microbiome was determined mainly by environmental conditions, rather than genotype. Bacterial likely contributed volatile profiles. Particularly, candidate operational taxonomic units (OTUs) in genus Sphingomonas highly correlated with C6 aldehyde volatiles (also called green leaf volatiles, GLVs), which fresh taste. Furthermore, core set expressed genes enriched lipid metabolism, responsible for bacterial colonization synthesis activation. Finally, similar profile observed after inoculating skin two sp., thus providing evidence hypothetical microbe–metabolite relationship. These results provide novel insight into how environment–microbiome–plant quality (E × Mi Q) interaction may thereby typicality, serving foundation decision‐making vineyard microbial management.

Language: Английский

Citations

0
Improving rice drought tolerance through host-mediated microbiome selection DOI Creative Commons
Alex Styer, Dean Pettinga, Daniel Caddell

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 5, 2024

Abstract Plant microbiome engineering remains a significant challenge due to challenges associated with accurately predicting assembly and function in complex, heterogeneous soil environments. However, host-mediated selection can simplify the process by using plant host phenotype as reporter of function; iteratively selecting microbiomes from hosts desired phenotypes them inoculate subsequent cohorts hosts, artificial steer towards composition producing optimized phenotypes. In this study, we inoculated rice wild microbial communities fallow field, desert, serpentine seep field soils. By challenging these plants drought least stressed across multiple generations, derived simplified that enhanced both growth tolerance rice. Across cycles, within between treatments became increasingly similar, implicating dispersal drivers community composition. With amplicon sequencing data identified specific bacterial taxa improved phenotypes; while many have been previously described promoters, also novel exhibiting strong positive correlation performance. Lastly, resolved 272 metagenome-assembled genomes (MAGs) used MAGs identify functions enriched bacteria driving tolerance. The most significantly functions—particularly glycerol-3-phosphate iron transport—have implicated potential mediators plant-microbe interactions during drought. Altogether, demonstrate provides an efficient framework for through identification individual

Language: Английский

Citations

3
Contrasting roles of abundant and rare root-associated fungi in wheat: Community assembly, heritability and agronomic impacts DOI

Xia Kang,

Yuyin Zheng,

Zhihan Feng

et al.

Applied Soil Ecology, Journal Year: 2025, Volume and Issue: 209, P. 106032 - 106032

Published: March 15, 2025

Language: Английский

Citations

0
The plant microbiota: From theoretical advances to applications DOI Creative Commons
Weidong Liu, Haoran Xu, Jinghang Zhou

et al.

Fundamental Research, Journal Year: 2024, Volume and Issue: unknown

Published: April 1, 2024

Language: Английский

Citations

2
Compositional profiling of the rhizosphere microbiome of Canada thistle reveals consistent patterns across the United States northern Great Plains DOI Creative Commons
Jed O. Eberly,

Asa Hurd,

Dipiza Oli

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Aug. 4, 2024

Canada thistle is a pervasive perennial weed, causing challenges to agricultural and natural ecosystems globally. Although research has focused on the phenology, genetics, control of thistle, little known about rhizosphere microbiome role plant–microbe interactions play in invasion success. This study investigated across diverse climates, soils, crops U.S. northern Great Plains. Soil samples were collected bacterial 16S fungal ITS2 sequencing performed characterize core identify potential factors contributing Amplicon revealed stable that was detected all locations. The dominated by phyla Actinobacteriota Proteobacteria Ascomycota Basidiomycota. Differential abundance analysis showed communities enriched pathogen-containing genera with 1.7-fold greater Fusaria 2.6-fold Gibberella compared bulk soil. Predictive functional profiling (p < 0.05, FDR corrected) plant pathogen guilds which represented 19% community. similar composition environments, highlighting association between specific microbial taxa. characterized findings highlight shaping invasive behavior. These are important for understanding ecological impacts soil-microbe processes.

Language: Английский

Citations

2
Genotype-associated core bacteria enhance host resistance against kiwifruit bacterial canker DOI Creative Commons
Min Fu,

Chen Yun-he,

Yongxin Liu

et al.

Horticulture Research, Journal Year: 2024, Volume and Issue: 11(11)

Published: Aug. 13, 2024

Abstract Both the phyllosphere and rhizosphere are inhabited by different kinds of microorganisms that closely related to plant growth health. However, it is not clear whether disease-resistant cultivars shape microbiome facilitate disease resistance. In this study, significant differences were found in aboveground belowground bacterial communities disease-susceptible grown same kiwifruit orchard. The resistant cultivar ‘Wanjin’ showed greater enrichment Pseudomonas spp. Sphingomonas than susceptible ‘Donghong’. microbes less affected field location, with significantly abundance those ‘Donghong’ more bacteria potential biocontrol properties. syringae pv. actinidiae (Psa) infection plants, especially Resistant exhibit distinct beneficial microbial recruitment strategies under Psa challenge. Jinzhai was enriched Pantoea infection, while Novosphingobium We further identified five key biomarkers within community associated infection. Inoculation experiments Lysobacter sp. R34, Stenotrophomonas R31, R10 RS54, which isolated from compartments ‘Wanjin’, could positively affect performance combination use R31 improve management canker. Our findings provided novel insights into soil–microbe–plant interactions role resistance susceptibility.

Language: Английский

Citations

2
When the microbiome meets One Health principle: Leading to the Holy Grail of biology and contributing to overall well‐being and social sustainability DOI Creative Commons

Yazhou Zhou,

Zhenguo Xu, Huan Zhang

et al.

iMetaOmics., Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 10, 2024

Abstract The convergence of microbiome science with the One Health principle heralds a transformative era in biology, prioritizing collective well‐being humans, animals, and environment. This review delves into intricate dance between microbiomes their hosts, revealing profound impact on health, nutrient cycles, climate change. Championing unified approach to health issues across diverse kingdoms life, emerges as holistic strategy, underscored by proposed universal balance theory, “Balance Dynamic Factors.” theory spotlights equilibrium within microbial human‐animal‐environment interactions, offering revolutionary pathway global social well‐being. It paves way for disease prevention, equity, sustainability, all which are purviews balanced ecological system. We navigate challenges opportunities this integrative approach, culminating call action incorporation policies, precision medicine, legislation, eco‐health projects, education, thereby setting stage harmonious coexistence our planet.

Language: Английский

Citations

2
A Multiplexing Activity-Based Protein-Profiling Platform for Dissection of a Native Bacterial Xyloglucan-Degrading System DOI Creative Commons
Nicholas G. S. McGregor, Casper de Boer, Quentin Foucart

et al.

ACS Central Science, Journal Year: 2023, Volume and Issue: 9(12), P. 2306 - 2314

Published: Nov. 24, 2023

Bacteria and yeasts grow on biomass polysaccharides by expressing excreting a complex array of glycoside hydrolase (GH) enzymes. Identification annotation such GH pools, which are valuable commodities for sustainable energy chemistries, conventional means (genomics, proteomics) complicated, as primary sequence or secondary structure alignment with known active enzymes is not always predictive new ones. Here we report "low-tech", easy-to-use, sensitive multiplexing activity-based protein-profiling platform to characterize the xyloglucan-degrading system excreted soil saprophyte, Cellvibrio japonicus, when grown xyloglucan. A suite probes bearing orthogonal fluorophores allows visualization accessory exo-acting glycosidases, then identified using biotin-bearing probes. Substrate specificity xyloglucanases directly revealed imbuing xyloglucan structural elements into bespoke Our ABPP provides highly useful tool dissect systems from various sources rapidly select potentially The observed moreover bodes well study other polysaccharide-degrading systems, modeling probe structures those desired substrates.

Language: Английский

Citations

6
Improving rice drought tolerance through host-mediated microbiome selection DOI Open Access
Alex Styer, Dean Pettinga, Daniel Caddell

et al.

Published: May 8, 2024

Plant microbiome engineering remains a significant challenge due to challenges associated with accurately predicting assembly and function in complex, heterogeneous soil environments. However, host-mediated selection can simplify the process by using plant host phenotype as reporter of function; iteratively selecting microbiomes from hosts desired phenotypes them inoculate subsequent cohorts hosts, artificial steer towards composition producing optimized phenotypes. In this study, we inoculated rice wild microbial communities fallow field, desert, serpentine seep field soils. By challenging these plants drought least stressed across multiple generations, derived simplified that enhanced both growth tolerance rice. Across cycles, within between treatments became increasingly similar, implicating dispersal drivers community composition. With amplicon sequencing data identified specific bacterial taxa improved phenotypes; while many have been previously described promoters, also novel exhibiting strong positive correlation performance. Lastly, resolved 272 metagenome-assembled genomes (MAGs) used MAGs identify functions enriched bacteria driving tolerance. The most significantly functions—particularly glycerol-3-phosphate iron transport—have implicated potential mediators plant-microbe interactions during drought. Altogether, demonstrate provides an efficient framework for through identification individual

Language: Английский

Citations

1