Improving rice drought tolerance through host-mediated microbiome selection

Published: May 8, 2024

Plant microbiome engineering remains a significant challenge due to challenges associated with accurately predicting assembly and function in complex, heterogeneous soil environments. However, host-mediated selection can simplify the process by using plant host phenotype as reporter of function; iteratively selecting microbiomes from hosts desired phenotypes them inoculate subsequent cohorts hosts, artificial steer towards composition producing optimized phenotypes. In this study, we inoculated rice wild microbial communities fallow field, desert, serpentine seep field soils. By challenging these plants drought least stressed across multiple generations, derived simplified that enhanced both growth tolerance rice. Across cycles, within between treatments became increasingly similar, implicating dispersal drivers community composition. With amplicon sequencing data identified specific bacterial taxa improved phenotypes; while many have been previously described promoters, also novel exhibiting strong positive correlation performance. Lastly, resolved 272 metagenome-assembled genomes (MAGs) used MAGs identify functions enriched bacteria driving tolerance. The most significantly functions—particularly glycerol-3-phosphate iron transport—have implicated potential mediators plant-microbe interactions during drought. Altogether, demonstrate provides an efficient framework for through identification individual

Language: Английский

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The impact of space, host dissimilitude, and environment on prokaryotic communities of golf ball sponges

Marine Ecology, Journal Year: 2024, Volume and Issue: 45(5)

Published: June 20, 2024

Abstract Golf ball sponges are small, sometimes inconspicuous, sponges. They can be found across a range of habitats varying from perturbed and pristine coral reefs to harbours marine lakes the deep sea shallow waters. difficult distinguish in field have presented some problems with taxonomists lumping splitting species due difficulty defining clear boundaries. In present study, we sampled golf Indo‐Pacific Caribbean locations used 16S gene amplicon sequencing study their prokaryotic communities. We show that harbour wide variety Among most prevalent operational taxonomic units (OTUs), several belonged taxa, including bacterial AqS1 EC94 groups, which been associated genes known facilitate interactions between hosts microbes. Certain host taxa were enriched OTUs classified SAR202 clade Chloroflexi. Our findings dissimilarity varied as function space (geographical distance) dissimilitude. The importance dissimilitude, however, depending on data transformation dissimilitude more important predictor untransformed whereas was log‐transformed data. Given log‐transformation downscales influence abundant interpret these results by tendency closely related organisms similar sets symbiotic microorganisms; distantly specimens, contrast, tend less microorganisms surrounding environment (e.g., seawater or sediment).

Language: Английский

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Genotype of pioneer plant Miscanthus is not a key factor in the structure of rhizosphere bacterial community in heavy metal polluted sites

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 477, P. 135242 - 135242

Published: July 17, 2024

Language: Английский

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Understanding plant responsiveness to microbiome feedbacks

Current Opinion in Plant Biology, Journal Year: 2024, Volume and Issue: 81, P. 102603 - 102603

Published: July 17, 2024

Plant microbiome interactions are bidirectional with processes leading to assembly and effects on plants, so called feedbacks. With belowground focus we systematically decomposed both of these directions into plant (root rhizosphere) components identify methodological challenges research priorities. We found that the bidirectionality presents a challenge for genetic studies. Establishing causality is particularly difficult when mutant has both, an altered phenotype microbiome. Is mutation directly affecting (e.g., through root exudates), which then causes and/or consequence altering plant's architecture)? Here, put forward feedback experiments allow separate cause effect furthermore, they useful investigating complex microbiomes in natural soils. They especially investigate basis how plants respond soil stress such feedbacks understudied compared mechanisms contributing assembly. Thinking towards application, this may develop crops abilities assemble beneficial actively exploit its

Language: Английский

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Shifts in fungal community diversity and potential function under natural forest succession and planted forest restoration in the Kunyu Mountains, East China

Ecology and Evolution, Journal Year: 2024, Volume and Issue: 14(8)

Published: Aug. 1, 2024

Abstract Soil fungi participate in various ecosystem processes and are important factors driving the restoration of degraded forests. However, little is known about changes fungal diversity potential functions under development different vegetation types during natural (secondary forest succession) anthropogenic (reforestation) restoration. In this study, we selected typical succession sequences (including Pinus densiflora Siebold & Zucc., pine‐broadleaf mixed P. Quercus acutissima Carruth., Q. ), as well secondary deciduous broadleaved forests planted Robinia pseudoacacia on Kunyu Mountain for analysis. We used ITS rRNA gene sequencing to characterize communities FUNGuild database predict functional groups. The results showed that affected β‐diversity, but not α‐diversity. There was a significant increase Basidiomycota decrease Ascomycota later successional stage, accompanied by an groups ectomycorrhizal (ECM). Conversely, exhibited decreased α‐diversity altered community compositions, characterized fewer more Mucoromycota. Planted led relative abundances ECM animal pathogens. TK content major factor explaining distinction among three stages, whereas pH, AP, NH 4 + were variations between Changes significantly soil reforestation, providing key insights management temperate

Language: Английский

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