Detection of the Fungus Batrachochytrium dendrobatidis in Anurans from the Semiarid Region of Brazil: new Infection Records for Endemic Species DOI
Márcio Mendes, Francisca Andréa da Silva Oliveira, Luzia Gabrielle Zeferino de Castro

et al.

South American Journal of Herpetology, Journal Year: 2024, Volume and Issue: 33(3)

Published: Dec. 30, 2024

Chytridiomycosis, caused by the fungus Batrachochytrium dendrobatidis (Bd), has affected amphibians globally since 1970s, being considered main cause of their decline worldwide. The present study is first to assess presence Bd in anuran populations dry and wet tropical forests Ceará state, northeastern Brazil. Samples were collected different environmental landscapes throughout dominant Caatinga relict moist forest (brejos de altitude) localities Monte Alegre Private Natural Heritage Reserve, Ibiapaba Plateau, Serra Maranguape, Araripe Farias Brito, Campos Sales. harbor amphibian species typical open areas, while possess endemic potentially threatened species. a peculiar Brazilian biome that been neglected scientific research conservation efforts, highlighting importance our study. Anuran skin samples from adults using swabs, DNA was extracted amplified PCR Bd-specific primers. In total, 125 analyzed, comprising 28 eight families, with 20 (71%) sampled testing positive for Bd. This record on infection Adenomera juikitam, Boana raniceps, Dendropsophus nanus, D. soaresi, Leptodactylus troglodytes, L. vastus, Physalaemus cicada, Pristimantis relictus, Proceratophrys ararype, Pseudopaludicola mystacalis Scinax x-signatus. Particularly Bd-positive ararype relictus can be as greatest concern, they recently described known distribution restricted high elevation relictual forests.

Language: Английский

Leveraging machine learning to uncover multi-pathogen infection dynamics across co-distributed frog families DOI Creative Commons
Daniele L. F. Wiley,

Kadie N. Omlor,

Ariadna S. Torres López

et al.

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e18901 - e18901

Published: Jan. 29, 2025

Background Amphibians are experiencing substantial declines attributed to emerging pathogens. Efforts understand what drives patterns of pathogen prevalence and differential responses among species challenging because numerous factors related the host, pathogen, their shared environment can influence infection dynamics. Furthermore, sampling across broad taxonomic geographic scales evaluate these poses logistical challenges, interpreting roles multiple potentially correlated variables is difficult with traditional statistical approaches. In this study, we leverage frozen tissues stored in natural history collections machine learning techniques characterize dynamics three generalist pathogens known cause mortality frogs. Methods We selected 12 widespread abundant focal taxa within ecologically distinct, co-distributed host families (Bufonidae, Hylidae, Ranidae) sampled them eastern two-thirds United States America. screened quantified loads via quantitative PCR for major pathogens: fungal Batrachochytrium dendrobatidis (Bd), double-stranded viruses lineage Ranavirus (Rv), alveolate parasite currently referred as Amphibian Perkinsea (Pr). then built balanced random forests (RF) models predict status intensity based on taxonomy, age, sex, geography, environmental assess relative variable importance Lastly, used one-way analyses determine directional relationships significance identified predictors. Results found approximately 20% individuals were infected at least one (231 single infections 25 coinfections). The most prevalent all groups was Bd (16.9%; 95% CI [14.9–19%]), followed by Rv (4.38%; [3.35–5.7%]) Pr (1.06%; [0.618–1.82%]). highest family Ranidae, which represented 74.3% infections, including majority points, had significantly higher intensities compared Bufonidae Hylidae. Host temperature key predictors RF models, differences families. For Rv, associated latitudes cooler, more stable temperatures, while showed trends opposite direction. no significant between sexes, but juvenile frogs adults. Overall, our study highlights use a strategy identifying important multi-host, multi-pathogen systems.

Language: Английский

Citations

0
Host life stage susceptibility to the chytrid fungus in a Neotropical torrent frog DOI
Julia R. Ernetti, Joelma S. Prado, Luı́s Felipe Toledo

et al.

Fungal Biology, Journal Year: 2025, Volume and Issue: unknown, P. 101546 - 101546

Published: Feb. 1, 2025

Language: Английский

Citations

0
Environmental screening through nanopore native sequencing leads to the detection of Batrachochytrium dendrobatidis in La Mandria Regional Park, Italy. DOI Creative Commons
Amir Reza Varzandi, Tim Reska, Lara Urban

et al.

Global Ecology and Conservation, Journal Year: 2025, Volume and Issue: unknown, P. e03517 - e03517

Published: Feb. 1, 2025

Language: Английский

Citations

0
Editorial: From landscape modifications to pathogen infections: are threats to amphibians the same in all biomes? DOI Creative Commons
Joice Ruggeri, David Lesbarrères

Frontiers in Amphibian and Reptile Science, Journal Year: 2025, Volume and Issue: 3

Published: March 6, 2025

Language: Английский

Citations

0
Genotypic Discrimination of Chytrid Fungus Lineages in the Amphibian Trade DOI
Luisa P. Ribeiro, David Rodríguez, Roseli Coelho dos Santos

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
Genotypic discrimination of chytrid fungus lineages in the amphibian trade DOI Open Access
Luisa P. Ribeiro, David Rodríguez,

Roseli La Corte dos Santos

et al.

Authorea (Authorea), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 2, 2024

The international amphibian trade raises concerns regarding its impact on native populations due to the potential introduction of different lineages chytrid fungus (Batrachochytrium dendrobatidis or Bd) into new environments. Current diagnostic methods, particularly qPCR, lack capability differentiate between these lineages, necessitating more accurate genotyping approaches. Here we propose application TaqMan SNP assays discriminate Bd genotypes (Bd-GPL, Bd-ASIA2/Bd-BRAZIL [hereafter Bd-BRAZIL], and Hybrid) in samples from Brazilian bullfrog farms. We collected samples, including skin swabs, tadpole mouthparts, pure cultures, farms across Brazil. Employing two utilizing both qPCR dPCR, identified genotype presence analyzed relationship load determination. assay achieved a 56.6 % success rate, notably improving with higher loads, reaching 81.8 for loads over 1,000 genomic equivalents. Culture 100 rate. all farms, highlighting issue coinfections hybrids densely populated hereby present an efficient method discriminating genotypes, applicable cultures field low loads. emphasize need advanced discriminatory methods comprehensive genetic studies, national regulations governing breeding sites global trade. Our research underscores feasibility significance proposed advocates further investigations infection dynamics by inform conservation efforts regulatory policies.

Language: Английский

Citations

0
Detection of the Fungus Batrachochytrium dendrobatidis in Anurans from the Semiarid Region of Brazil: new Infection Records for Endemic Species DOI
Márcio Mendes, Francisca Andréa da Silva Oliveira, Luzia Gabrielle Zeferino de Castro

et al.

South American Journal of Herpetology, Journal Year: 2024, Volume and Issue: 33(3)

Published: Dec. 30, 2024

Chytridiomycosis, caused by the fungus Batrachochytrium dendrobatidis (Bd), has affected amphibians globally since 1970s, being considered main cause of their decline worldwide. The present study is first to assess presence Bd in anuran populations dry and wet tropical forests Ceará state, northeastern Brazil. Samples were collected different environmental landscapes throughout dominant Caatinga relict moist forest (brejos de altitude) localities Monte Alegre Private Natural Heritage Reserve, Ibiapaba Plateau, Serra Maranguape, Araripe Farias Brito, Campos Sales. harbor amphibian species typical open areas, while possess endemic potentially threatened species. a peculiar Brazilian biome that been neglected scientific research conservation efforts, highlighting importance our study. Anuran skin samples from adults using swabs, DNA was extracted amplified PCR Bd-specific primers. In total, 125 analyzed, comprising 28 eight families, with 20 (71%) sampled testing positive for Bd. This record on infection Adenomera juikitam, Boana raniceps, Dendropsophus nanus, D. soaresi, Leptodactylus troglodytes, L. vastus, Physalaemus cicada, Pristimantis relictus, Proceratophrys ararype, Pseudopaludicola mystacalis Scinax x-signatus. Particularly Bd-positive ararype relictus can be as greatest concern, they recently described known distribution restricted high elevation relictual forests.

Language: Английский

Citations

0