Stress Changes the Bacterial Biomolecular Condensate Material State and Shifts Function from mRNA Decay to Storage DOI Open Access
Luis A. Ortiz‐Rodríguez, Hadi M. Yassine, Vidhyadhar Nandana

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 12, 2024

Abstract Bacterial ribonucleoprotein bodies (BR-bodies) are dynamic biomolecular condensates that play a pivotal role in RNA metabolism. We investigated how BR-bodies significantly influence mRNA fate by transitioning between liquid- and solid-like states response to stress. With combination of single-molecule bulk fluorescence microscopy, biochemical assays, quantitative analyses, we determine promote efficient decay liquid-like condensate during exponential growth. On the other hand, repurposed from sites reservoirs for storage under stress, functional change is enabled their transition more rigid state, marked reduced internal dynamics, increased molecular density, prolonged residence time ribonuclease E. Furthermore, manipulated ATP levels translation rates conclude accumulation ribosome-depleted key factor driving these material state transitions, maturation further contributes this process. Upon nutrient replenishment, stationary-phase disassemble, releasing stored mRNAs rapid translation, demonstrating BR-body function governed reversible mechanism resource management. These findings reveal adaptive strategies which bacteria regulate metabolism through condensate-mediated control storage.

Language: Английский

Biomolecular condensates as stress sensors and modulators of bacterial signaling DOI Creative Commons
Moeka Sasazawa, Dylan T. Tomares, W. Seth Childers

et al.

PLoS Pathogens, Journal Year: 2024, Volume and Issue: 20(8), P. e1012413 - e1012413

Published: Aug. 15, 2024

Microbes exhibit remarkable adaptability to environmental fluctuations. Signaling mechanisms, such as two-component systems and secondary messengers, have long been recognized critical for sensing responding cues. However, recent research has illuminated the potential of a physical adaptation mechanism in signaling—phase separation, which may represent ubiquitous compartmentalizing biochemistry within cytoplasm context bacteria that frequently lack membrane-bound organelles. This review considers broader prospect phase separation play roles rapid stress response mechanisms pathogens. It is well established weak multivalent interactions between disordered regions, coiled-coils, other structured domains can form condensates via be regulated by specific parameters some cases. The process itself acts responsive sensor, influenced changes protein concentration, posttranslational modifications, temperature, salts, pH, oxidative stresses. environmentally triggered can, turn, regulate functions recruited biomolecules, providing stressful conditions. As examples, we describe biochemical pathways organized are essential cell physiology signaling features. These include proteins organize modify chromosome (Dps, Hu, SSB), decay, modification RNA (RNase E, Hfq, Rho, polymerase), those involved signal transduction (PopZ, PodJ, SpmX) (aggresomes polyphosphate granules). We also summarize pathogens function challenges targeting biomolecular next-generation antimicrobial therapeutics. Together, this illuminates emerging significance microbial signaling, responses, regulation provides framework microbiologists consider diverse

Language: Английский

Citations

6
Editorial overview: Rise of the bacterial nanomachines DOI
Susan Schlimpert, Martin Thanbichler

Current Opinion in Microbiology, Journal Year: 2024, Volume and Issue: 80, P. 102515 - 102515

Published: July 21, 2024

Language: Английский

Citations

0
Stress Changes the Bacterial Biomolecular Condensate Material State and Shifts Function from mRNA Decay to Storage DOI Open Access
Luis A. Ortiz‐Rodríguez, Hadi M. Yassine, Vidhyadhar Nandana

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 12, 2024

Abstract Bacterial ribonucleoprotein bodies (BR-bodies) are dynamic biomolecular condensates that play a pivotal role in RNA metabolism. We investigated how BR-bodies significantly influence mRNA fate by transitioning between liquid- and solid-like states response to stress. With combination of single-molecule bulk fluorescence microscopy, biochemical assays, quantitative analyses, we determine promote efficient decay liquid-like condensate during exponential growth. On the other hand, repurposed from sites reservoirs for storage under stress, functional change is enabled their transition more rigid state, marked reduced internal dynamics, increased molecular density, prolonged residence time ribonuclease E. Furthermore, manipulated ATP levels translation rates conclude accumulation ribosome-depleted key factor driving these material state transitions, maturation further contributes this process. Upon nutrient replenishment, stationary-phase disassemble, releasing stored mRNAs rapid translation, demonstrating BR-body function governed reversible mechanism resource management. These findings reveal adaptive strategies which bacteria regulate metabolism through condensate-mediated control storage.

Language: Английский

Citations

0