Effects of Difenoconazole and Imidacloprid Seed Coatings on Soil Microbial Community Diversity and Ecological Function DOI Creative Commons

Dunfeng Feng,

Jiabin Chen,

Guo Li

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 806 - 806

Published: April 1, 2025

Difenoconazole and imidacloprid are key components of seed-coating agents, which alter soil microbial community structure function after application. Existing studies mainly focus on the environmental effects their spraying application, while research impacts ecosystem when used as agents is relatively limited. Through field experiments, this study systematically evaluated compared difenoconazole seed coatings wheat rhizosphere communities ecological functions by measuring enzyme activities, employing 16S rRNA ITS high-throughput sequencing technologies predicting KEGG functional pathways. The results showed that significantly reduced bacterial diversity, particularly under high-dosage treatment (0.18 g a.i./kg seed), with a 5.80% decrease in diversity day 30. This most strongly inhibited phyla Bacteroidota Myxococcota, maximum reductions 23.87% 63.57%, respectively. However, abundance Actinobacteriota increased, increase 38.53%. Additionally, fungal increased both treatments. Both altered from days 20 to 60, recovery occurring 120. Furthermore, pathway analysis revealed seed) activated pathways such cell motility, signal transduction, membrane transport, whereas standard dosage (0.12 exhibited metabolic suppression. elucidates dynamic agent application communities, providing theoretical support for rational pesticide use optimization agricultural strategies.

Language: Английский

Dibohemamines I–O from Streptomyces sp. GZWMJZ-662, an endophytic actinomycete from the medicinal and edible plant Houttuynia cordata Thunb. DOI Creative Commons
Dongyang Wang, Mingxing Li, Yanchao Xu

et al.

Natural Products and Bioprospecting, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 6, 2025

Abstract A chemical investigation of Streptomyces sp. GZWMJZ-662, an endophytic actinomycete isolated from Houttuynia cordata Thunb., has yielded eleven bohemamine dimers ( 1 – 11 ). Notably, the newly identified dibohemamines I–O 7 ) have not been previously reported. Their structures were elucidated through detailed spectroscopic analysis, encompassing high-resolution electrospray ionization mass, nuclear magnetic resonance, infrared radiation, ultraviolet–visible, and electronic circular dichroism spectroscopy. Dibohemamine I exhibited selective cytotoxic effects against cancer cell lines 786-O GBC-SD among 18 evaluated, with half-inhibitory concentration values 3.24 ± 0.20 7.36 0.41 μM, respectively. Graphical

Language: Английский

Citations

0
Geliboluols A–D: Kaurane-Type Diterpenoids from the Marine-Derived Rare Actinomycete Actinomadura geliboluensis DOI Creative Commons

Chang-Su Heo,

Jong Soon Kang,

Jeong-Wook Yang

et al.

Marine Drugs, Journal Year: 2025, Volume and Issue: 23(2), P. 78 - 78

Published: Feb. 10, 2025

Four new kaurane-type diterpenoids, geliboluols A-D (1-4), along with one known analog (5), were isolated from the culture broth of marine-derived rare actinomycete Actinomadura geliboluensis. The structures compounds 1-4 determined by spectroscopic analysis (HR-ESIMS, 1D, and 2D NMR), MPA method, comparing their optical rotation values those in literature. evaluated for cytotoxicity against seven blood cancer cell lines a CellTiter-Glo (CTG) assay six solid sulforhodamine B (SRB) assay. Among compounds, compound 4 exhibited moderate cytotoxic activity some lines, GI50 ranging 2.59 to 19.64 µM, 4.34 7.23 µM.

Language: Английский

Citations

0
Effects of Difenoconazole and Imidacloprid Seed Coatings on Soil Microbial Community Diversity and Ecological Function DOI Creative Commons

Dunfeng Feng,

Jiabin Chen,

Guo Li

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 806 - 806

Published: April 1, 2025

Difenoconazole and imidacloprid are key components of seed-coating agents, which alter soil microbial community structure function after application. Existing studies mainly focus on the environmental effects their spraying application, while research impacts ecosystem when used as agents is relatively limited. Through field experiments, this study systematically evaluated compared difenoconazole seed coatings wheat rhizosphere communities ecological functions by measuring enzyme activities, employing 16S rRNA ITS high-throughput sequencing technologies predicting KEGG functional pathways. The results showed that significantly reduced bacterial diversity, particularly under high-dosage treatment (0.18 g a.i./kg seed), with a 5.80% decrease in diversity day 30. This most strongly inhibited phyla Bacteroidota Myxococcota, maximum reductions 23.87% 63.57%, respectively. However, abundance Actinobacteriota increased, increase 38.53%. Additionally, fungal increased both treatments. Both altered from days 20 to 60, recovery occurring 120. Furthermore, pathway analysis revealed seed) activated pathways such cell motility, signal transduction, membrane transport, whereas standard dosage (0.12 exhibited metabolic suppression. elucidates dynamic agent application communities, providing theoretical support for rational pesticide use optimization agricultural strategies.

Language: Английский

Citations

0