Anin vivofitness gene ofToxoplasma, MIC11, is essential for PLP1-mediated egress from host cells DOI Creative Commons
Yuta Tachibana, Miwa Sasai, Hidetaka Kosako

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Summary After invasion and replication, intracellular pathogens must egress from infected host cells . Toxoplasma gondii facilitates this process by permeabilizing releasing perforin-like protein 1 (PLP1) through induced microneme secretion. However, the precise mechanism of cell permeabilization remains enigmatic. Here, we identified secretory MIC11 as a key factor for membrane disruption. A CRISPR-based in vivo screen revealed several genes including an essential gene virulence. Deletion resulted severe defects both rupture egress. Scanning mutagenesis functional motifs MIC11, mechanistic analyses demonstrated that directly associates with PLP1, regulating its activity The paralogue MIC22 compensated deletion, suggesting conserved feline-restricted stages T. discovery advances understanding how parasites disrupt to facilitate rapid successful dissemination.

Language: Английский

Anin vivofitness gene ofToxoplasma, MIC11, is essential for PLP1-mediated egress from host cells DOI Creative Commons
Yuta Tachibana, Miwa Sasai, Hidetaka Kosako

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Summary After invasion and replication, intracellular pathogens must egress from infected host cells . Toxoplasma gondii facilitates this process by permeabilizing releasing perforin-like protein 1 (PLP1) through induced microneme secretion. However, the precise mechanism of cell permeabilization remains enigmatic. Here, we identified secretory MIC11 as a key factor for membrane disruption. A CRISPR-based in vivo screen revealed several genes including an essential gene virulence. Deletion resulted severe defects both rupture egress. Scanning mutagenesis functional motifs MIC11, mechanistic analyses demonstrated that directly associates with PLP1, regulating its activity The paralogue MIC22 compensated deletion, suggesting conserved feline-restricted stages T. discovery advances understanding how parasites disrupt to facilitate rapid successful dissemination.

Language: Английский

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