Specificities of Chemosensory Receptors in the Human Gut Microbiota DOI Creative Commons
Wenhao Xu, Ekaterina Jalomo-Khayrova, Vadim M. Gumerov

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 11, 2025

The human gut is rich in metabolites and harbors a complex microbial community, yet the sensory repertoire of its commensal bacteria remains largely uncharacterized. Here we systematically mapped ligand specificities extracytoplasmic domains from twenty members microbiota, with primary focus on abundant physiologically important class Clostridia. We identified diverse as specific stimuli for three major functional classes transmembrane receptors. further characterized novel subsets sensors belonging to Cache superfamily, lactate, dicarboxylic acids, uracil short-chain fatty acids (SCFAs), respectively, investigated evolution their specificity. Structural biochemical analysis newly described dCache_1UR domain revealed an independent binding SCFA at distinct modules. Altogether, could identify or predict over half Cache-type chemotactic selected commensals, carboxylic representing largest ligands. Among those, most commonly found were lactate formate, indicating particular importance these microbiome consistent observed beneficial impact growth bacterial species.

Language: Английский

Specificities of Chemosensory Receptors in the Human Gut Microbiota DOI Creative Commons
Wenhao Xu, Ekaterina Jalomo-Khayrova, Vadim M. Gumerov

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 11, 2025

The human gut is rich in metabolites and harbors a complex microbial community, yet the sensory repertoire of its commensal bacteria remains largely uncharacterized. Here we systematically mapped ligand specificities extracytoplasmic domains from twenty members microbiota, with primary focus on abundant physiologically important class Clostridia. We identified diverse as specific stimuli for three major functional classes transmembrane receptors. further characterized novel subsets sensors belonging to Cache superfamily, lactate, dicarboxylic acids, uracil short-chain fatty acids (SCFAs), respectively, investigated evolution their specificity. Structural biochemical analysis newly described dCache_1UR domain revealed an independent binding SCFA at distinct modules. Altogether, could identify or predict over half Cache-type chemotactic selected commensals, carboxylic representing largest ligands. Among those, most commonly found were lactate formate, indicating particular importance these microbiome consistent observed beneficial impact growth bacterial species.

Language: Английский

Citations

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