Gallic acid-doped multifunctional hybrid hydrogel for antioxidant and antibacterial studies

European Polymer Journal, Journal Year: 2024, Volume and Issue: 206, P. 112778 - 112778

Published: Jan. 18, 2024

Language: Английский

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Exploring the Potential of Chitosan–Phytochemical Composites in Preventing the Contamination of Antibiotic-Resistant Bacteria on Food Surfaces: A Review

Molecules, Journal Year: 2025, Volume and Issue: 30(3), P. 455 - 455

Published: Jan. 21, 2025

The escalating presence of antibiotic-resistant bacteria (ARB) in food systems presents a pressing challenge, particularly preventing contamination and ensuring safety. Traditional sanitation methods, such as cooking chemical disinfectants, provide effective means to reduce ARB, yet there is growing need for additional preventive measures directly on surfaces. This review explores the potential chitosan–phytochemical composites (CPCs) surface coatings prevent initial by thereby offering novel complementary approach conventional safety practices. Chitosan, combined with active plant-derived metabolites (phytochemicals), forms notable antibacterial antioxidant properties that enhance its protective effects. We examine CPC synthesis methodologies, including modifications, free radical-induced grafting, enzyme-mediated techniques, which stability activity CPCs against ARB. Highlighting recent findings CPCs’ efficacy through minimum inhibitory concentrations (MIC) zones inhibition, this underscores ARB risks surfaces, seafood, meat, postharvest products. insights provided here aim encourage future strategies leveraging preventative treatment mitigate production processing environments.

Language: Английский

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Gallic acid exerts antibiofilm activity by inhibiting methicillin-resistant Staphylococcus aureus adhesion

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: July 26, 2024

Methicillin-resistant Staphylococcus aureus (MRSA) is a serious threat to patients with nosocomial infections, and infection strongly associated biofilm formation. Gallic acid (GA) natural bioactive compound found in traditional Chinese medicines that exerts potent antimicrobial activity. However, the anti-MRSA efficacy of GA remained be determined. This study investigated activities against MRSA mechanisms involved. The results revealed significant antibacterial antibiofilm GA. minimal inhibitory concentration was 32 μg/mL growth curve assay confirmed effect on planktonic MRSA. Crystal violet XTT assays showed 8 µg/mL effectively inhibited formation new biofilms disrupted existing by reducing both biomass metabolic activities. Alkaline phosphatase β-galactosidase leakage live/dead staining provided evidence integrity bacterial cell walls membranes within biofilm. Scanning electron microscopy observations significantly adhesion aggregation, affecting overall structure Bacterial adhesion, polysaccharide intercellular (PIA) production real-time quantitative PCR PIA synthesis, expression icaAD sarA. These suggested inhibiting sarA, then downregulating icaA icaD, thereby synthesis attenuate capacity therefore promising candidate for development as pharmaceutical agent prevention treatment infections caused

Language: Английский

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Green-synthesized silver nanoparticles from Centella asiatica and Ayapana triplinervis: A novel approach to treating wound infections and reducing antimicrobial resistance

South African Journal of Botany, Journal Year: 2025, Volume and Issue: 177, P. 617 - 629

Published: Jan. 5, 2025

Language: Английский

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Green Tea Extract (Theaceae; Camellia sinensis L.): A Promising Antimicrobial, Anti-Quorum Sensing and Antibiofilm Candidate Against Multidrug-Resistant Campylobacter Species

Antibiotics, Journal Year: 2025, Volume and Issue: 14(1), P. 61 - 61

Published: Jan. 9, 2025

Thermophilic Campylobacter species are among the main culprits behind bacterial gastroenteritis globally and have grown progressively resistant to clinically important antimicrobials. Many studies been carried out explore innovative alternative strategies control antibiotic-resistant campylobacters in animal reservoirs human hosts; however, limited performed develop efficient schemes against biofilms. This study investigated antimicrobial antibiofilm activities of some herbal extracts multidrug-resistant (MDR) recovered from different sources using phenotypic molecular techniques. The overall prevalence was 21.5%, representing 15.25% 6.25% for C. jejuni coli, respectively. Regarding jejuni, highest resistance rate observed amoxicillin-clavulanic acid colistin (85.25% each), followed by cefotaxime (83.61%) tetracycline (81.97%), whereas coli isolates showed absolute erythromycin (92%) (88%). Remarkably, all were MDR with elevated multiple (MAR) indices (0.54-1). potentials green tea (Camellia sinensis), rosemary (Rosmarinus officinalis) ginger (Zingiber officinale) assessed disk diffusion assay broth microdilution technique. Green extract a marked inhibitory effect tested isolates, exhibiting growth inhibition zone diameters 8 38 mm minimum concentration (MIC) range 1.56-3.12 mg/mL, unlike extracts. Our findings reveal respectable activity (>50% biofilm formation inhibition) preformed biofilms isolates. Furthermore, real-time quantitative polymerase chain reaction (RT-qPCR) results significant decrease (p < 0.05) expression levels biosynthesis gene its regulator (FlaA LuxS, respectively) treated comparison untreated ones. is first vitro approach that has documented MDR-biofilm-producing isolated sources. Further vivo animals' models should be provide evidence concept implementation this candidate mitigation infections future.

Language: Английский

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Assessing the Potential of Gallic Acid and Methyl Gallate to Enhance the Efficacy of β-Lactam Antibiotics against Methicillin-Resistant Staphylococcus aureus by Targeting β-Lactamase: In Silico and In Vitro Studies

Antibiotics, Journal Year: 2023, Volume and Issue: 12(11), P. 1622 - 1622

Published: Nov. 13, 2023

Methicillin-resistant Staphylococcus aureus (MRSA), a global health concern, has prompted research into antibiotic adjuvants as potential solution. Although our group previously reported the enhancing effects of gallic acid (GA) and methyl gallate (MG) on penicillin G activity against MRSA, synergistic with other β-lactam antibiotics underlying mechanism have not been fully explored. Therefore, this study primarily aimed to investigate antibacterial synergism through disc diffusion, checkerboard, time–kill assays. The β-lactamase inhibition was also examined both molecular modeling in vitro experiments. Additionally, bacterial morphology changes were studied using scanning electron microscopy (SEM). results revealed that GA MG exhibited anti-MRSA showed indifferent when combined methicillin susceptible S. (MSSA). Interestingly, demonstrated only β-lactamase-unstable MRSA lowest fractional inhibitory concentration (FIC) indexes ≤3.75. However, weak inhibition. Furthermore, GA, MG, combination ampicillin induced morphological suggesting possible affecting cell membrane. These findings suggest could potentially serve an adjunct combat infections.

Language: Английский

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Antibacterial Effect of Gallic Acid in UV-C Light Treatment Against Escherichia coli O157:H7 and the Underlying Mechanism

Food and Bioprocess Technology, Journal Year: 2023, Volume and Issue: 17(8), P. 2216 - 2231

Published: Nov. 14, 2023

Language: Английский

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NADES systems comprising choline chloride and polyphenols: Physicochemical characterization, antioxidant and antimicrobial activities

Journal of Molecular Liquids, Journal Year: 2024, Volume and Issue: 410, P. 125683 - 125683

Published: Aug. 3, 2024

Language: Английский

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A Comprehensive Review of Antimicrobial Agents Against Clinically Important Bacterial Pathogens: Prospects for Phytochemicals

Phytotherapy Research, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 4, 2024

ABSTRACT Antimicrobial resistance (AMR) hinders the effective treatment of a range bacterial infections, posing serious threat to public health globally, as it challenges currently available antimicrobial drugs. Among various modes action, agents that act on membranes have most promising efficacy. However, there are no consolidated reports shortcomings these drugs, existing challenges, or potential applications phytochemicals membranes. Therefore, in this review, we addressed and focused acting clinically important pathogens. Antibacterial comprise diverse group found wide plants. These compounds been disrupt cell membranes, inhibit enzymes, interfere with protein synthesis, generate reactive oxygen species, modulate quorum sensing, adhesion, making them candidates for development novel antibacterial therapies. Recently, polyphenolic reported proven efficacy against nosocomial multidrug‐resistant more high‐quality studies, improved standards, adoption rules regulations required firmly confirm clinical derived from Identifying thrust areas research, considering viable approaches is essential successful translation compounds.

Language: Английский

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Phytochemical Profiling, Antioxidant and Antimicrobial Potentials of Ethanol and Ethyl Acetate Extracts of Chamaenerion latifolium L.

Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(8), P. 996 - 996

Published: July 27, 2024

The study investigates the phytochemical profile, antioxidant capacity, and antimicrobial activities of ethanol (ChL-EtOH) ethyl acetate (ChL-EtOAc) extracts from

Language: Английский

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