Research Square (Research Square),
Journal Year:
2023,
Volume and Issue:
unknown
Published: July 26, 2023
Abstract
Background:
Non-alcoholic
steatohepatitis
(NASH)
has
become
a
major
public
health
issue.
In
fact,
it
is
one
of
the
leading
causes
disturbed
liver
function
and
transplantation
worldwide.
Its
link
with
intestinal
microbiota
still
topical,
production
endogenous
ethanol
following
dysbiosis
-
mechanisms
linked
to
disease
been
shown
in
several
studies.
this
study,
we
analyzed
stool
samples
from
41
NASH
patients
24
healthy
controls
using
culturomics
16S
rRNA
targeted
metagenomics
identify
microbial
profile
associated
each
group.
Fecal
concentration
was
also
determined
for
all
samples.
Results:
We
identified
358
different
bacterial
species
approach
distributed
into
11
phyla
143
genera.
With
significant
difference,
12
appeared
significantly
more
frequently
group
4
control
Finegoldia
magna
most
detected
(10/14
(71%)),
2
producing
strains:
Limosilactobacillus
fermentum
Enterocloster
bolteae.
Metagenomics
shows
an
enrichment
Lactobacillus
Streptococcus
identical
that
culture.
regard
species,
there
elevated
frequency
ethanol-producing
bacteria
patients,
higher
stools
compared
(bilateral
Mann-Whitney
test,
p=value
0.0145).
However,
metagenomics,
only
culture
showed
bolteae
patients.
Conclusions:
Culturomics
are
complementary
methods
exploring
microbiota.
Our
results
suggest
may
play
instrumental
role
pathogenesis
disease.
Deleted Journal,
Journal Year:
2024,
Volume and Issue:
unknown
Published: July 1, 2024
This
study
provides
different
opinion
for
exploring
the
mechanism
of
catechin
(CAT)
relieving
nonalcoholic
steatohepatitis
(NASH),
it
is
more
innovative
to
explore
from
perspective
intestinal
microorganism.
Through
in
vitro
fermentation
experiments,
CAT
could
improve
abundance
Enterococcus,
and
Enterococcus
faecalis
(EF)
accounts
vast
majority
Enterococcus
in
human
gut.
The
experimental
results
vivo
showed
that
EF
group
CAT+EF
reduce
body
weight,
liver
weight
epididymal
fat
NASH
mice,
changes
serum
indexes.
HE
staining
observation
these
two
groups
have
greatly
improved
fatty
degeneration,
balloon
degeneration
necrotic
focus
caused
by
NASH.
alleviation
was
obvious.
Results
targeted
metabonomics
promote
produce
methyl
palmitate
(MP,
C16:0),
which
plays
a
great
role
Our
indicated
alleviate
had
better
may
due
production
MP
EF.
new
idea
Research Square (Research Square),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Oct. 3, 2023
Abstract
Background
Studies
of
the
relationship
between
human
microbiota
and
host,
particularly
in
maintenance
health
onset
disease,
is
still
a
topical
issue.
The
home
to
number
microorganisms,
including
bacteria,
yeasts,
viruses
archaea,
which
play
crucial
role
body's
proper
functioning.
In
presence
risk
factors,
mainly
antibiotics,
unbalanced
diet
so
on,
disequilibrium
known
as
dysbiosis
can
set
in,
eventually
leading
several
diseases.
Several
mechanisms
have
been
described
for
this
dysbiosis,
but
one
linked
endogenous
ethanol
production
under-investigated.
Results
aim
review
provide
an
inventory
all
ethanol-producing
microorganisms
humans.
Over
36,0000,000
articles
were
searched
on
pubmed
with
our
query,
7275
retained.
Of
these,
5397
contributed
review,
enabling
us
identify
85
humans,
61
bacterial
species
24
yeasts.
Conclusion
Our
results
establish
important
certain
They
will
also
enable
better
understand
origins
diseases
mechanism,
improve
diagnosis
therapeutic
management
future.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(16), P. 8924 - 8924
Published: Aug. 16, 2024
The
global
burden
of
liver
cancer
is
increasing.
Timely
diagnosis
important
for
optimising
the
limited
available
treatment
options.
Understanding
metabolic
consequences
hepatocellular
carcinoma
(HCC)
may
lead
to
more
effective
We
aimed
document
metabolite
differences
between
HCC
and
matched
surrounding
tissues
varying
aetiology,
obtained
at
time
resection,
interpret
changes
with
clinical
findings.
High-resolution
magic
angle
spinning
nuclear
magnetic
resonance
(HRMAS-NMR)
spectroscopy
analyses
N
=
10
paired
non-tumour
tissue
samples
were
undertaken.
There
marked
HRMAS-NMR
in
lipid
levels
compared
subtle
low-molecular-weight
metabolites,
particularly
when
adjusting
patient-specific
variability.
Differences
lipid-CH3,
lipid-CH2,
formate,
acetate
particular
interest.
obvious
content
highlight
intricate
interplay
adaptations
cell
survival
complex
microenvironment
cancer.
formate
might
relate
bacterial
metabolites.
Therefore,
documentation
metabolites
according
histology
findings
patients
interest
personalised
medicine
approaches
tailoring
targeted
strategies.
Research Square (Research Square),
Journal Year:
2023,
Volume and Issue:
unknown
Published: July 26, 2023
Abstract
Background:
Non-alcoholic
steatohepatitis
(NASH)
has
become
a
major
public
health
issue.
In
fact,
it
is
one
of
the
leading
causes
disturbed
liver
function
and
transplantation
worldwide.
Its
link
with
intestinal
microbiota
still
topical,
production
endogenous
ethanol
following
dysbiosis
-
mechanisms
linked
to
disease
been
shown
in
several
studies.
this
study,
we
analyzed
stool
samples
from
41
NASH
patients
24
healthy
controls
using
culturomics
16S
rRNA
targeted
metagenomics
identify
microbial
profile
associated
each
group.
Fecal
concentration
was
also
determined
for
all
samples.
Results:
We
identified
358
different
bacterial
species
approach
distributed
into
11
phyla
143
genera.
With
significant
difference,
12
appeared
significantly
more
frequently
group
4
control
Finegoldia
magna
most
detected
(10/14
(71%)),
2
producing
strains:
Limosilactobacillus
fermentum
Enterocloster
bolteae.
Metagenomics
shows
an
enrichment
Lactobacillus
Streptococcus
identical
that
culture.
regard
species,
there
elevated
frequency
ethanol-producing
bacteria
patients,
higher
stools
compared
(bilateral
Mann-Whitney
test,
p=value
0.0145).
However,
metagenomics,
only
culture
showed
bolteae
patients.
Conclusions:
Culturomics
are
complementary
methods
exploring
microbiota.
Our
results
suggest
may
play
instrumental
role
pathogenesis
disease.
