Pseudomonas aeruginosa : metabolic allies and adversaries in the world of polymicrobial infections DOI Creative Commons
Chandni Sachdeva, Kapaettu Satyamoorthy,

Thokur Sreepathy Murali

et al.

Critical Reviews in Microbiology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 20

Published: Sept. 3, 2024

Pseudomonas aeruginosa (PA), an opportunistic human pathogen that is frequently linked with chronic infections in immunocompromised individuals, also metabolically versatile, and thrives diverse environments. Additionally, studies report PA can interact other microorganisms, such as bacteria, fungi, producing unique metabolites modulate the host immune response, contribute to disease pathogenesis. This review summarizes current knowledge related metabolic interactions of microorganisms (Staphylococcus, Acinetobacter, Klebsiella, Enterococcus, Candida) hosts, importance these a polymicrobial context. Further, we highlight potential applications studying toward designing better diagnostic tools, therapeutic strategies prevent, treat caused by this pathogen.

Language: Английский

Review on bacterial outer membrane vesicles: structure, vesicle formation, separation and biotechnological applications DOI Creative Commons
Xiaofei Zhao,

Yusen Wei,

Yuqing Bu

et al.

Microbial Cell Factories, Journal Year: 2025, Volume and Issue: 24(1)

Published: Jan. 21, 2025

Outer membrane vesicles (OMVs), shed by Gram-negative bacteria, are spherical nanostructures that play a pivotal role in bacterial communication and host-pathogen interactions. Comprising an outer envelope encapsulating variety of bioactive molecules from their progenitor OMVs facilitate material informational exchange. This review delves into the recent advancements OMV research, providing comprehensive overview structure, biogenesis, mechanisms vesicle formation. It also explores pathogenicity techniques for enrichment isolation. Furthermore, highlights burgeoning applications field biomedicine, emphasizing potential as diagnostic tools, vaccine candidates, drug delivery vectors.

Language: Английский

Citations

2
Inhibiting NLRP3 enhances cellular autophagy induced by outer membrane vesicles from Pseudomonas aeruginosa DOI Creative Commons

Jing Ge,

Min Cao, Yuyao Zhang

et al.

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

ABSTRACT The bacterium Pseudomonas aeruginosa is able to invade lung epithelial cells and survive intracellularly. During this process, it secretes outer membrane vesicles (OMVs), however, currently unclear how OMVs from P. (PA-OMVs) affect their impact on oxidative stress, autophagy, other physiological activities of cells. In study, we found that PA-OMVs activated stress autophagy in We demonstrated the NLRP3 (NLR family, pyrin domain containing 3) inhibitor MCC950 can enhance induced by PA-OMVs. main function related body’s immune response inflammation regulation. most common NLRP3. Additionally, showed not only enhanced expression AMP-activated protein kinase, a key regulator cellular energy homeostasis, reactive oxygen species, which play crucial role signaling but also significantly Inhibiting further process autophagy. These results demonstrate activate both inflammasome, with suppressing certain extent, hoping provide broad ideas for future applications IMPORTANCE discovery exposed induce protective immunity significant. Specifically, addition an inhibitor, MCC950, has been decrease targets while simultaneously enhancing activity This finding unveils novel theoretical framework development vaccines, highlighting new anti-infective responses. By elucidating mechanisms through trigger bolster defenses, research opens avenues innovative vaccine design strategies aimed at combatting infections effectively.

Language: Английский

Citations

1
Ultrasound-enhanced dual-responsive bismuth nanocatalysts for alleviating tumor hypoxia and promoting breast cancer sonodynamic-immunotherapy DOI
Junjie Pan, Hong Qian, Jiajia Zheng

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 160292 - 160292

Published: Feb. 1, 2025

Language: Английский

Citations

1
Insights into Bacterial Extracellular Vesicle Biogenesis, Functions, and Implications in Plant–Microbe Interactions DOI Creative Commons

Sarita Pandey,

Anaïs Blache, Wafa Achouak

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(3), P. 532 - 532

Published: March 6, 2024

Plant–microbe interactions play a crucial role in shaping plant health and survival. In recent years, the of extracellular vesicles (EVs) mediating intercellular communication between plants microbes has emerged as an intriguing area research. EVs serve important carriers bioactive molecules genetic information, facilitating cells even different organisms. Pathogenic bacteria leverage to amplify their virulence, exploiting cargo rich toxins virulence factors. Conversely, beneficial initiate EV secretion stimulate immune responses nurture symbiotic relationships. The transfer EV-packed small RNAs (sRNAs) been demonstrated facilitate modulation responses. Furthermore, harnessing potential holds promise for development innovative diagnostic tools sustainable crop protection strategies. This review highlights biogenesis functions importance defense, paves way future research this exciting field.

Language: Английский

Citations

4
Outer membrane vesicles in gram-negative bacteria and its correlation with pathogenesis DOI Creative Commons
F Abolhasani,

Nasim Vaghefinanekaran,

Aref Yarahmadi

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 1, 2025

There is a widespread distribution of gram-negative bacteria worldwide, which are responsible for the deaths numerous patients each year. The illnesses they cause can be localized and systemic, these possess several key virulence factors that contribute to their pathogenicity. In recent years, distinct mechanisms pathogenesis have evolved remain largely unknown scientists medical experts. Among these, outer membrane vesicles (OMVs) undoubtedly one most significant influencing virulence. OMVs contain various bacterial compounds diverse effects on host organisms immune system, potentially exacerbating disease inflammation while evading responses. This review comprehensively examines role in pathogenesis, interaction with cells, potential biomedical applications. Understanding molecular governing OMV biogenesis function could pave way novel antimicrobial strategies therapeutic interventions.

Language: Английский

Citations

0
Therapeutic Potential and Translational Challenges for Bacterial Extracellular Vesicles in Inflammatory Bowel Disease DOI
Nicholas H. Pirolli,

Jean-Pierre Raufman,

Steven M. Jay

et al.

Inflammatory Bowel Diseases, Journal Year: 2025, Volume and Issue: unknown

Published: May 13, 2025

Abstract Despite the availability of numerous new immune-directed therapeutics, major constituents inflammatory bowel disease (IBD)—ulcerative colitis (UC) and Crohn’s (CD)—continue to afflict millions worldwide, resulting in significant morbidity long-term health risks. IBD results from a triad immune, environmental (eg, gut microbiome), genetic (including epigenetic) mechanisms, therefore has been subject wide variety therapeutic strategies. Among these, administration probiotics, particularly Gram-positive lactic acid bacteria (LAB), targeting both immune factors, shown promising potential for efficacy selected populations early clinical trials. However, knowledge gaps inconsistent currently prevent recommendations use probiotics larger patient populations. The is likely due variable cell viability potency after administration, further exacerbated by heterogeneity. Thus, an alternative live emerged form bacterial extracellular vesicles (BEVs)—cell-secreted nanovesicles containing abundant bioactive cargo that, like can regulate factors but with fewer limitations safety concerns. In this review, we summarize work done date establishing BEVs provide benefits discuss hurdles must overcome achieve translation. We also consider future directions BEV especially treatment necrotizing enterocolitis (NEC), which shares similarities pathophysiology IBD.

Language: Английский

Citations

0
Extracellular Vesicles and Bacterial Infection DOI
Nivedita Koley, Hemanta Koley

Published: Jan. 1, 2024

Citations

0
Outer membrane vesicles from commensal microbes contribute to the sponge Tedania sp. development by regulating the expression level of apoptosis-inducing factor (AIF) DOI Creative Commons
Kai Wang,

Chenzheng Jia,

Beibei Zhang

et al.

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: Aug. 6, 2024

The transition from the swimming larval stage to settlement represents a significant node in marine sponge developmental process. Previous research has shown that outer membrane vesicles (OMVs) bacterial species Tenacibaculum mesophilum associated with Tedania sp. influence settlement: low concentrations of OMVs increase attachment rate, whereas high decrease rate. Here, by comparing transcriptomes larvae filtered seawater (FSW group) and FSW supplemented (FSW-OMV group), results indicated affected modulating expression levels apoptosis-inducing factor (AIF) host. Subsequently, quantitative real-time PCR revealed aif near time (SE) compared control group. RNA interference (RNAi) was used target gene, rate significantly reduced, confirming inhibitory effect OMVs. Moreover, small (sRNA) sequencing existence abundant AIF-sRNAs 30 nt, further suggesting one pathway for involvement sponge-associated bacteria host development is transport direct function cargo loading. A comparative transcriptome study on suggests promoted sp., were regulate apoptosis transporting symbiotic microorganism.

Language: Английский

Citations

0
Pseudomonas aeruginosa : metabolic allies and adversaries in the world of polymicrobial infections DOI Creative Commons
Chandni Sachdeva, Kapaettu Satyamoorthy,

Thokur Sreepathy Murali

et al.

Critical Reviews in Microbiology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 20

Published: Sept. 3, 2024

Pseudomonas aeruginosa (PA), an opportunistic human pathogen that is frequently linked with chronic infections in immunocompromised individuals, also metabolically versatile, and thrives diverse environments. Additionally, studies report PA can interact other microorganisms, such as bacteria, fungi, producing unique metabolites modulate the host immune response, contribute to disease pathogenesis. This review summarizes current knowledge related metabolic interactions of microorganisms (Staphylococcus, Acinetobacter, Klebsiella, Enterococcus, Candida) hosts, importance these a polymicrobial context. Further, we highlight potential applications studying toward designing better diagnostic tools, therapeutic strategies prevent, treat caused by this pathogen.

Language: Английский

Citations

0