Preliminary study on Cyclocodon lancifolius leaf blight and screening of Bacillus subtilis as a biocontrol agent DOI Creative Commons
Xin Yang, Youchao Dang,

Jing-Zhong Chen

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 15, 2024

Introduction This study aimed to identify the pathogen responsible for leaf blight in Cyclocodon lancifolius , investigate its biological characteristics, and effective synthetic fungicides. Additionally, this examined changes physiological biochemical indices of leaves following infection screened biocontrol bacteria that inhibit growth, providing a scientific basis preventing managing C. . Methods Pathogens were isolated from interface healthy infected tissues identified through morphological molecular methods. Amplification sequencing three genomic DNA regions—internal transcribed spacer region, translation elongation factor 1-α, glyceraldehyde-3-phosphate dehydrogenase ribosomal DNA—were performed, followed by construction phylogenetic tree. The characteristics pathogens under various temperature pH conditions different nitrogen carbon sources analyzed using mycelial growth rate method. antifungal effects 13 chemical agents evaluated poisoned medium method Changes indicators post-infection also assessed. An antagonistic experiment was conducted screen bacteria. Results A total 29 potential pathogenic strains tissues, with Koch’s Postulates confirming Stemphylium lycopersici as key causing disease. Growth analysis S. revealed optimal at 20°C 6, lactose or maltose serving most suitable source histidine preferred source. Among fungicides tested, strain DHY4 exhibited greatest sensitivity 400 g/L flusilazole. Significant differences ( p < 0.05) observed superoxide dismutase, phenylalanine ammonia-lyase, peroxidase, polyphenol oxidase, catalase, malondialdehyde levels between treated control groups 3 days post-inoculation. DYHS2, Bacillus subtilis demonstrated an inhibition 51.80% against dual-culture experiments showed relative 78.82% detached assays. Discussion These findings provide valuable insights into newly causal agent underscoring B. DYHS2 such flusilazole disease management strategies.

Language: Английский

Biostimulant and Beyond: Bacillus spp., the Important Plant Growth-Promoting Rhizobacteria (PGPR)-Based Biostimulant for Sustainable Agriculture DOI
Wenli Sun, Mohamad Hesam Shahrajabian

Earth Systems and Environment, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 2, 2025

Language: Английский

Citations

3
Antimicrobial Activity of Bacillus Cyclic Lipopeptides and Their Role in the Host Adaptive Response to Changes in Environmental Conditions DOI Open Access
Н. Н. Маркелова,

Anastasia Chumak

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(1), P. 336 - 336

Published: Jan. 2, 2025

Bacillus cyclic lipopeptides (CLP), part of the three main families—surfactins, iturins, and fengycins—are secondary metabolites with a unique chemical structure that includes both peptide lipid components. Being amphiphilic compounds, CLPs exhibit antimicrobial activity in vitro, damaging membranes microorganisms. However, concentrations used vitro are difficult to achieve natural conditions. Therefore, environment, alternative mechanisms action by more likely, such as inducing apoptosis fungal cells, preventing microbial adhesion substrate, promoting death phytopathogens stimulating plant immune responses. In addition, low act signaling molecules Bacillus’s own metabolism, when environmental conditions change, they form an adaptive response host bacterium. Namely, trigger differentiation bacterial population into various specialized cell types: competent flagellated matrix producers, spores. this review, we have summarized current understanding under experimental We also shown relationship between some regulatory pathways involved CLP biosynthesis differentiation, well role determine changes physiological state subpopulations shifts

Language: Английский

Citations

0
Comparison and Analysis of Resistance Differences in Alternaria alternata from Fungicides with Three Different Mechanisms DOI Creative Commons

Quanjiang Bai,

Xinbo Ma,

M. Hayat

et al.

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(4), P. 305 - 305

Published: April 11, 2025

The pathogen Alternaria alternata infects a variety of plants and crops, notably poplars, results in large financial losses. Using twelve chemical fungicides for fungicide sensitivity tests (FSTs) on A. alternata, the result showed that prochloraz (PCZ), mancozeb (MZ), fludioxonil (FLU) have potent inhibitory effects against through different mechanisms. To investigate how responded to fungicide-induced stress, transcriptome physiological investigations were carried out after treatments with three at their corresponding 50% effective concentration (EC50) doses. MZ treatment produced distinct genetic response; FLU greatest number differentially expressed genes (DEGs), followed by PCZ. DEGs from mostly engaged ribosome biosynthesis, those lipid carbohydrate metabolism, PCZ according Gene Ontology (GO) analysis. Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment analysis revealed associated biogenesis, whereas was linked pyruvate metabolic pathway. Collinear trend indicates exhibits unique pattern, causing most significant overexpression genes, six categories 88 elevated fungal resistance include tyrosinase, ATP-binding cassette (ABC) transporters, major facilitator superfamily (MFS) antioxidant cellular resilience as well involved cell wall membrane biosynthesis. Notably, pathways synthesis melanin ergosterol exhibited strongest response FLU. correlation between indices resistance-related indicated content, malondialdehyde (MDA) tyrosinase activity positively correlated majority DEGs, soluble protein superoxide dismutase (SOD) activity, catalase (CAT) negatively correlated, which is consistent observed trends measured indicators. Taken together, this study provides theoretical basis developing more control strategies alternata.

Language: Английский

Citations

0
Agro Active Potential of Bacillus subtilis PE7 against Didymella bryoniae (Auersw.), the Causal Agent of Gummy Stem Blight of Cucumis melo DOI Creative Commons

Seo Kyoung Jeong,

Seong Eun Han,

Prabhakaran Vasantha‐Srinivasan

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(8), P. 1691 - 1691

Published: Aug. 16, 2024

Microbial agents such as the

Language: Английский

Citations

1
Genomic Insights into the Bactericidal and Fungicidal Potential of Bacillus mycoides b12.3 Isolated in the Soil of Olkhon Island in Lake Baikal, Russia DOI Creative Commons
Maria N. Romanenko, Anton E. Shikov, Iuliia A. Savina

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(12), P. 2450 - 2450

Published: Nov. 28, 2024

The dispersal of plant pathogens is a threat to the global economy and food industry which necessitates need discover efficient biocontrol agents such as bacteria, fungi, etc., inhibiting them. Here, we describe Bacillus mycoides strain b12.3 isolated from soil Olkhon Island in Lake Baikal, Russia. By applying co-cultivation technique, found that inhibits growth pathogens, bacteria Xanthomonas campestris, Clavibacter michiganensis, Pectobacterium atrospecticum, well fungus Alternaria solani. To elucidate genomic fundament explaining these activities, leveraged next-generation whole-genome sequencing obtained high-quality assembly based on short reads. isolate bore seven known BGCs (biosynthetic gene clusters), including those responsible for producing bacillibactin, fengycin, petrobactin. Moreover, genome contained insecticidal genes encoding App4Aa1, Tpp78Ba1, Spp1Aa1 toxins, thus implicating possible pesticidal potential. We compared with 50 closest assemblies enriched BGCs. analysis also revealed architecture corresponds experimentally observed activity spectrum implying combination produced secondary metabolites delineates range inhibited phytopathogens Therefore, this study deepens our knowledge biology ecology B. residing Baikal region.

Language: Английский

Citations

0
Preliminary study on Cyclocodon lancifolius leaf blight and screening of Bacillus subtilis as a biocontrol agent DOI Creative Commons
Xin Yang, Youchao Dang,

Jing-Zhong Chen

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 15, 2024

Introduction This study aimed to identify the pathogen responsible for leaf blight in Cyclocodon lancifolius , investigate its biological characteristics, and effective synthetic fungicides. Additionally, this examined changes physiological biochemical indices of leaves following infection screened biocontrol bacteria that inhibit growth, providing a scientific basis preventing managing C. . Methods Pathogens were isolated from interface healthy infected tissues identified through morphological molecular methods. Amplification sequencing three genomic DNA regions—internal transcribed spacer region, translation elongation factor 1-α, glyceraldehyde-3-phosphate dehydrogenase ribosomal DNA—were performed, followed by construction phylogenetic tree. The characteristics pathogens under various temperature pH conditions different nitrogen carbon sources analyzed using mycelial growth rate method. antifungal effects 13 chemical agents evaluated poisoned medium method Changes indicators post-infection also assessed. An antagonistic experiment was conducted screen bacteria. Results A total 29 potential pathogenic strains tissues, with Koch’s Postulates confirming Stemphylium lycopersici as key causing disease. Growth analysis S. revealed optimal at 20°C 6, lactose or maltose serving most suitable source histidine preferred source. Among fungicides tested, strain DHY4 exhibited greatest sensitivity 400 g/L flusilazole. Significant differences ( p < 0.05) observed superoxide dismutase, phenylalanine ammonia-lyase, peroxidase, polyphenol oxidase, catalase, malondialdehyde levels between treated control groups 3 days post-inoculation. DYHS2, Bacillus subtilis demonstrated an inhibition 51.80% against dual-culture experiments showed relative 78.82% detached assays. Discussion These findings provide valuable insights into newly causal agent underscoring B. DYHS2 such flusilazole disease management strategies.

Language: Английский

Citations

0