Phage Endolysins as Promising and Effective Candidates for Use Against Uropathogenic Escherichia coli DOI Creative Commons
Wojciech Wesołowski, Aleksandra Łukasiak, Sylwia Bloch

et al.

Viruses, Journal Year: 2025, Volume and Issue: 17(4), P. 560 - 560

Published: April 13, 2025

The presented in silico and phylogenetic analysis of putative endolysins potentially produced by phages infecting uropathogenic Escherichia coli (UPEC) demonstrates their remarkable diversity. These proteins exhibit significant variations sequence length, molecular weight, isoelectric point, stability, as well diverse functional domains determining enzymatic activity, including lysin, lysozyme, hydrolase, amidase, peptidase functions. Due to predicted lytic properties, hold great promise combating UPEC bacteria, those within biofilms, which are often highly resistant conventional treatments. Despite potential, several challenges hinder the full utilization endolysins. include relatively small number identified proteins, annotation process, scarcity studies evaluating efficacy vitro vivo against Gram-negative bacteria. In this work, we emphasize these while also underlining potential an effective tool infections. Their effectiveness could be significantly enhanced when combined with agents that disrupt outer membrane making them a promising alternative or complement existing antimicrobial strategies. Further research is necessary fully explore therapeutic potential.

Language: Английский

Phage‐derived proteins: Advancing food safety through biocontrol and detection of foodborne pathogens DOI Creative Commons
Dahee Choi,

Sangryeol Ryu,

Minsuk Kong

et al.

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2025, Volume and Issue: 24(2)

Published: Feb. 3, 2025

Abstract The emergence of antimicrobial‐resistant foodborne pathogens poses a continuous health risk and economic burden as they can easily spread through contaminated food. Therefore, the demand for new antimicrobial agents to address this problem is steadily increasing. Similarly, development rapid, sensitive, accurate pathogen detection tools prerequisite ensuring food safety. Phage‐derived proteins have become innovative combating these because their potent activity host specificity. Phage are relatively free from regulation compared phages per se, there no concerns about transduction harmful genes. With recent progress in next‐generation sequencing technology, analysis phage genomes has more accessible, numerous with potential biocontrol been identified. This review provides comprehensive overview protein research on safety 2006 present, pivotal period marked by certification Generally Recognized As Safe (GRAS). Emphasizing advancements, we investigated diverse applications various purposes. While highlighting successful implementation proteins, also current bottlenecks propose strategies overcome challenges. By summarizing state phage‐derived contributes deeper understanding effective detecting pathogens.

Language: Английский

Citations

1
Phage Endolysins as Promising and Effective Candidates for Use Against Uropathogenic Escherichia coli DOI Creative Commons
Wojciech Wesołowski, Aleksandra Łukasiak, Sylwia Bloch

et al.

Viruses, Journal Year: 2025, Volume and Issue: 17(4), P. 560 - 560

Published: April 13, 2025

The presented in silico and phylogenetic analysis of putative endolysins potentially produced by phages infecting uropathogenic Escherichia coli (UPEC) demonstrates their remarkable diversity. These proteins exhibit significant variations sequence length, molecular weight, isoelectric point, stability, as well diverse functional domains determining enzymatic activity, including lysin, lysozyme, hydrolase, amidase, peptidase functions. Due to predicted lytic properties, hold great promise combating UPEC bacteria, those within biofilms, which are often highly resistant conventional treatments. Despite potential, several challenges hinder the full utilization endolysins. include relatively small number identified proteins, annotation process, scarcity studies evaluating efficacy vitro vivo against Gram-negative bacteria. In this work, we emphasize these while also underlining potential an effective tool infections. Their effectiveness could be significantly enhanced when combined with agents that disrupt outer membrane making them a promising alternative or complement existing antimicrobial strategies. Further research is necessary fully explore therapeutic potential.

Language: Английский

Citations

0