Synergistic Activity of Vancomycin and Gentamicin Against Staphylococcus aureus Biofilms on Polyurethane Surface DOI Creative Commons
Nícolas Henrique Borges, Paula Hansen Suss, Gabriel Burato Ortis

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(5), P. 1119 - 1119

Published: May 13, 2025

Background: Staphylococcus aureus are frequently associated with biofilm formation on intravascular devices. Biofilms limit antimicrobial penetration and promote phenotypic resistance, challenging conventional treatment strategies. Vancomycin (VAN) gentamicin (GEN) have been used clinically, but their combined antibiofilm activity remains underexplored. Objectives: This study evaluates the efficacy of VAN GEN, alone in combination, against biofilms formed by methicillin-resistant (MRSA) methicillin-sensitive S. (MSSA) polyurethane. Methods: MICs were determined for GEN. Biofilm biomass metabolic quantified using crystal violet MTT assays, respectively. viability was assessed through fluorescence microscopy a modified Calgary Device. A continuous-flow peristaltic model developed to test under simulated catheter conditions. Results: While monotherapy or GEN had modest effects, combination significantly reduced activity. 20 mg/L + 8 40 achieved over 70% reduction MRSA complete eradication MBEC assays. Dynamic assays confirmed therapy. Conclusion: The VAN/GEN exhibits synergistic aureus, particularly MRSA. These findings support its potential application salvage strategies, including antibiotic lock

Language: Английский

Phenolic Compounds from Pyrus communis Residues: Mechanisms of Antibacterial Action and Therapeutic Applications DOI Creative Commons
Jessica Ribeiro, Vanessa Silva, Gilberto Igrejas

et al.

Antibiotics, Journal Year: 2025, Volume and Issue: 14(3), P. 280 - 280

Published: March 8, 2025

Background/Objectives: The food industry produces substantial amounts of fruit byproducts, which are often discarded despite their high content bioactive compounds with potential therapeutic applications. Pyrus communis (pear) residues, particularly rich in phenolic compounds, represent a valuable yet underutilized resource. These byproducts have demonstrated significant antioxidant and antibacterial properties, suggesting for medical pharmaceutical This review aims to provide comprehensive analysis the profile P. emphasizing mechanisms prospective use combating oxidative stress resistance. Methods: A key from residues was conducted using ScienceDirect Google Scholar databases (from 2014 2024). Studies assessing activities were reviewed, focus on action against Gram-positive Gram-negative bacterial pathogens. Results: minimum 14 distinct identified among residues. However, chlorogenic acid catechin as primary contributors activity Hydroquinone exhibited strong effects through membrane disruption, enzyme inhibition, metabolic interference. Despite this potential, hydroquinone’s cytotoxicity regulatory concerns limit its direct application. Conclusions: While phenolics show promise natural agents, future research should address bioavailability, extraction standardization, safe formulation strategies. Investigating synergy conventional antibiotics improving stability cosmetic applications steps toward practical use. In vivo clinical studies crucial validating ensuring approval.

Language: Английский

Citations

0

Disarming Staphylococcus aureus: Review of Strategies Combating This Resilient Pathogen by Targeting Its Virulence DOI Creative Commons
Abdelaziz Touati, Nasir A. Ibrahim, Takfarinas Idres

et al.

Pathogens, Journal Year: 2025, Volume and Issue: 14(4), P. 386 - 386

Published: April 15, 2025

Staphylococcus aureus is a formidable pathogen notorious for its antibiotic resistance and diverse virulence mechanisms, including toxin production, biofilm formation, immune evasion. This article explores innovative anti-virulence strategies to disarm S. by targeting critical factors without exerting bactericidal pressure. Key approaches include inhibiting adhesion neutralizing toxins, disrupting quorum sensing (e.g., Agr system inhibitors), blocking iron acquisition pathways. Additionally, interventions two-component regulatory systems are highlighted. While promising, challenges such as strain variability, resilience, pharmacokinetic limitations, evolution underscore the need combination therapies advanced formulations. Integrating with traditional antibiotics host-directed offers sustainable solution combat multidrug-resistant aureus, particularly methicillin-resistant strains (MRSA), mitigate global public health crisis.

Language: Английский

Citations

0

Synergistic Activity of Vancomycin and Gentamicin Against Staphylococcus aureus Biofilms on Polyurethane Surface DOI Creative Commons
Nícolas Henrique Borges, Paula Hansen Suss, Gabriel Burato Ortis

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(5), P. 1119 - 1119

Published: May 13, 2025

Background: Staphylococcus aureus are frequently associated with biofilm formation on intravascular devices. Biofilms limit antimicrobial penetration and promote phenotypic resistance, challenging conventional treatment strategies. Vancomycin (VAN) gentamicin (GEN) have been used clinically, but their combined antibiofilm activity remains underexplored. Objectives: This study evaluates the efficacy of VAN GEN, alone in combination, against biofilms formed by methicillin-resistant (MRSA) methicillin-sensitive S. (MSSA) polyurethane. Methods: MICs were determined for GEN. Biofilm biomass metabolic quantified using crystal violet MTT assays, respectively. viability was assessed through fluorescence microscopy a modified Calgary Device. A continuous-flow peristaltic model developed to test under simulated catheter conditions. Results: While monotherapy or GEN had modest effects, combination significantly reduced activity. 20 mg/L + 8 40 achieved over 70% reduction MRSA complete eradication MBEC assays. Dynamic assays confirmed therapy. Conclusion: The VAN/GEN exhibits synergistic aureus, particularly MRSA. These findings support its potential application salvage strategies, including antibiotic lock

Language: Английский

Citations

0