Comprehensive Genomic Analysis of Klebsiella pneumoniae and Its Temperate N-15-like Phage: From Isolation to Functional Annotation DOI Creative Commons
Reham Yahya,

Aljawharah Albaqami,

Amal Alzahrani

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 908 - 908

Published: April 15, 2025

Antibiotic resistance to Klebsiella pneumoniae poses a major public health threat, particularly in intensive care unit (ICU) settings. The emergence of extensively drug-resistant (XDR) strains complicates treatment options, requiring deeper understanding their genetic makeup and potential therapeutic targets. This research delineated an strain obtained from ICU patient telomeric temperate phage derived hospital effluent. bacteria showed strong multiple antibiotics, including penicillin (≥16 μg/mL), ceftriaxone (≥32 meropenem (≥8 which was caused by SHV-11 beta-lactamase, NDM-1 carbapenemase, porin mutations (OmpK37, MdtQ). categorized as K46 O2a types carried virulence genes involved iron acquisition, adhesion, immune evasion, well plasmids (IncHI1B_1_pNDM-MAR, IncFIB) eleven prophage regions, reflecting its adaptability dissemination. 172,025 bp linear genome 46.3% GC content the N-15-like genomic similarities phages Sugarlandvirus genus, especially those that infect K. pneumoniae. There were structural proteins (11.8%), DNA replication repair enzymes (9.3%), toxin–antitoxin system (0.4%) encoded genome. A protelomerase ParA/B partitioning indicate is replicating maintaining itself manner similar N15 phage, renowned for plasmid throughout lysogeny. Understanding dynamics antibiotic pathogen development requires knowledge like this one, are known nature function altering bacterial profiles. regulatory also provide model into biology effects on microbial communities. importance genomes larger framework ecology evolution emphasized research.

Language: Английский

Comprehensive Genomic Analysis of Klebsiella pneumoniae and Its Temperate N-15-like Phage: From Isolation to Functional Annotation DOI Creative Commons
Reham Yahya,

Aljawharah Albaqami,

Amal Alzahrani

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 908 - 908

Published: April 15, 2025

Antibiotic resistance to Klebsiella pneumoniae poses a major public health threat, particularly in intensive care unit (ICU) settings. The emergence of extensively drug-resistant (XDR) strains complicates treatment options, requiring deeper understanding their genetic makeup and potential therapeutic targets. This research delineated an strain obtained from ICU patient telomeric temperate phage derived hospital effluent. bacteria showed strong multiple antibiotics, including penicillin (≥16 μg/mL), ceftriaxone (≥32 meropenem (≥8 which was caused by SHV-11 beta-lactamase, NDM-1 carbapenemase, porin mutations (OmpK37, MdtQ). categorized as K46 O2a types carried virulence genes involved iron acquisition, adhesion, immune evasion, well plasmids (IncHI1B_1_pNDM-MAR, IncFIB) eleven prophage regions, reflecting its adaptability dissemination. 172,025 bp linear genome 46.3% GC content the N-15-like genomic similarities phages Sugarlandvirus genus, especially those that infect K. pneumoniae. There were structural proteins (11.8%), DNA replication repair enzymes (9.3%), toxin–antitoxin system (0.4%) encoded genome. A protelomerase ParA/B partitioning indicate is replicating maintaining itself manner similar N15 phage, renowned for plasmid throughout lysogeny. Understanding dynamics antibiotic pathogen development requires knowledge like this one, are known nature function altering bacterial profiles. regulatory also provide model into biology effects on microbial communities. importance genomes larger framework ecology evolution emphasized research.

Language: Английский

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