Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Feb. 5, 2024
Abstract
Filamentous
fungi
produce
polysaccharide-degrading
enzymes
governing
tolerance
to
changing
environments,
and
this
is
controlled
by
a
poorly
understood
transcriptional
circuit.
Here,
genome-wide
screening
genetic
analyses
reveal
novel
regulatory
circuit
comprising
RsrC-RsrA-RsrB
that
positively
regulates
the
production
of
raw
starch-degrading
(RSDEs)
in
Penicillium
oxalicum.
Transcription
factor
(TF)
RsrA
essential
for
biosynthesis
RSDEs.
Two
TFs,
RsrB
RsrC,
containing
Zn2Cys6
C2H2
zinc
finger
domains,
respectively,
act
downstream
upstream
RsrA.
activates
transcription
rsrB,
three
nucleotides
(G-286,
G-287
G-292)
are
required
binding
rsrB.
Polypeptide
RsrB165-271
binds
conserved
DNA
sequence
5’-KBKWYSNRKNDVVBS-3’
promoters
genes
encoding
major
amylases.
RsrC
specifically
rsrA
promoter
via
bases
-850
-825,
but
not
amylase
genes.
This
influences
mycelial
growth
conidia
production.
The
findings
expand
complex
network
fungal
RSDE
development.
Journal of Fungi,
Journal Year:
2024,
Volume and Issue:
10(10), P. 697 - 697
Published: Oct. 7, 2024
species
have
been
reported
as
masters
in
producing
cellulolytic
enzymes
for
the
biodegradation
of
lignocellulolytic
biomass
and
biocontrol
agents
against
plant
pathogens
pests.
In
our
previous
study,
a
novel
