Scientific Reports,
Journal Year:
2023,
Volume and Issue:
13(1)
Published: May 26, 2023
In
this
study
GC-MS-based
untargeted
metabolomics
was
used
to
identify
the
metabolic
response
of
earthworm;
Eudrilus
eugeniae
exposed
sub-lethal
concentrations
chlorpyrifos-CHL,
cypermethrin-CYP,
Glyphosate-GLY,
and
Combined-C
(all
three
pesticides)
at
3,
6,
12
mg/kg.
Principal
component
analysis
obtained
datasets
revealed
a
clear
distinction
between
control
treatment
groups.
The
mean
weight
worms
in
treated
groups
decreased
significantly
(p
<
0.05).
Among
identified
metabolites,
oleic
acid
(~
93.47%),
lysine
92.20%),
glutamic
91.81%),
leucine
90.20%),
asparagine
94.20%),
methionine
92.27%),
malic
93.37%),
turanose
95.04%),
maltose
92.36%),
cholesta-3,5-diene
86.11%),
galactose
93.20%),
cholesterol
91.56%),
tocopherol
85.09%),
0.05),
whereas
myoinositol
83%)
isoleucine
(78.09%)
increased
0.05)
upon
exposure
CHL,
CYP,
GLY,
C.
Overall,
findings
suggest
that
earthworms
might
be
new
entry
point
for
pesticides
into
food
chain.
present
highlights
can
reliable
approach
understand
effect
different
xenobiotics
including
on
earthworms.
