Topical treatment of adult house flies, Musca domestica L. (Diptera: Muscidae), with Beauveria bassiana in combination with three entomopathogenic bacteria DOI Open Access
Dana M. Johnson, Emma N.I. Weeks, Eric D. LoVullo

et al.

Florida Entomologist, Journal Year: 2024, Volume and Issue: 107(1)

Published: Jan. 1, 2024

Abstract Biological control of house flies, Musca domestica L. (Diptera: Muscidae) has the potential to improve efficacy and sustainability integrated pest management. In a previous study, we demonstrated that three bacteria species ( Serratia marcescens Bizio [Enterobacteriales: Enterobacteriaceae], Photorhabdus temperata Fischer-Le Saux et al. Pseudomonas protegens Ramette [Pseudomonadales: Pseudomonadaceae]) are extremely virulent, inducing rapid morbidity high mortality in adult flies when injected into hemocoel but show little effect applied topically. Here tested hypothesis topical application these combination with entompathogenic fungus Beauveria bassiana (Bals. Criv.) Vuill. (Hypocreales: Cordycipitaceae) would result an increased if fungal penetration fly cuticle allowed for delivery hemocoel. Bacteria B. were either by at same time or sequential two pathogens 48 h apart. Results indicated evidence synergy between any bacterial pathogens. However, P. caused early (<50 % 3 days) alone . A modified disc diffusion assay had inhibitory effects on vegetative growth suggesting release antifungal molecules bacteria. sufficiently encouraging warrant further investigation this its associated toxins as biological tools.

Language: Английский

Unveiling the Microbiome Landscape: A Metagenomic Study of Bacterial Diversity, Antibiotic Resistance, and Virulence Factors in the Sediments of the River Ganga, India DOI Creative Commons
Ajaya Kumar Rout, Partha Sarathi Tripathy, Sangita Dixit

et al.

Antibiotics, Journal Year: 2023, Volume and Issue: 12(12), P. 1735 - 1735

Published: Dec. 14, 2023

The global rise in antibiotic resistance, fueled by indiscriminate usage medicine, aquaculture, agriculture, and the food industry, presents a significant public health challenge. Urban wastewater sewage treatment plants have become key sources of resistance proliferation. present study focuses on river Ganges India, which is heavily impacted human activities serves as potential hotspot for spread resistance. We conducted metagenomic analysis sediment samples from six distinct locations along to assess prevalence diversity genes (ARGs) within microbial ecosystem. revealed predominance Proteobacteria across regions Ganges. antimicrobial (AMR) virulence factors were determined various databases. In addition this, KEGG COG important pathways related AMR. outcomes highlight noticeable regional differences AMR genes. findings suggest that enhancing sanitation infrastructure could play crucial role mitigating impact This research contributes vital insights into environmental aspects highlighting importance targeted interventions fight against

Language: Английский

Citations

15
Plant communication with rhizosphere microbes can be revealed by understanding microbial functional gene composition DOI
Sandhya Mishra, Xianxian Zhang, Xiaodong Yang

et al.

Microbiological Research, Journal Year: 2024, Volume and Issue: 284, P. 127726 - 127726

Published: April 16, 2024

Language: Английский

Citations

4
Antimicrobial Resistance: What Lies Beneath This Complex Phenomenon? DOI Creative Commons

Giedrė Valdonė Sakalauskienė,

Aurelija Radzevičienė

Diagnostics, Journal Year: 2024, Volume and Issue: 14(20), P. 2319 - 2319

Published: Oct. 18, 2024

Antimicrobial Resistance (AMR) has evolved from a mere concern into significant global threat, with profound implications for public health, healthcare systems, and the economy. Since introduction of antibiotics between 1945 1963, their widespread often indiscriminate use in human medicine, agriculture, animal husbandry led to emergence rapid spread antibiotic-resistant genes. Bacteria have developed sophisticated mechanisms evade effects antibiotics, including drug uptake limitation, degradation, target modification, efflux pumps, biofilm formation, outer membrane vesicles production. As result, AMR now poses threat comparable climate change COVID-19 pandemic, projections suggest that death rates will be up 10 million deaths annually by 2050, along staggering economic cost exceeding $100 trillion. Addressing requires multifaceted approach, development new alternative therapies, shift antibiotic usage regulation. Enhancing surveillance increasing awareness, prioritizing investments research, diagnostics, vaccines are critical steps. By recognizing gravity committing collaborative action, its impact can mitigated, health protected future generations.

Language: Английский

Citations

4
The protective role of potassium in the adaptation of Pseudomonas protegens SN15-2 to hyperosmotic stress DOI
Jian Wang, Yaping Wang,

Shouquan Lu

et al.

Microbiological Research, Journal Year: 2024, Volume and Issue: 289, P. 127887 - 127887

Published: Aug. 30, 2024

Language: Английский

Citations

2
Role of the sigma factor AlgU in regulating growth, virulence, motility, exopolysaccharide production, and environmental stress adaptation of Pseudomonas syringae pv. actinidiae QSY6 DOI Creative Commons
Yiju Zhang, Min Fu,

Qian Wang

et al.

Phytopathology Research, Journal Year: 2024, Volume and Issue: 6(1)

Published: May 16, 2024

Abstract The extracytoplasmic function (ECF) sigma factor AlgU is involved in the regulation of various virulence-related pathways Pseudomonas syringae , especially alginate biosynthesis and motility, role differs among P. pathovars. However, to date, mechanism its virulence pv. actinidiae ( Psa ) still unclear. ECF factors are a class alternative that typically with anti-sigma as part cell-surface signaling systems. Under non-inducing conditions, remains inhibited by such MucA MucB. To investigate mutant strains lacking algU or mucA mucB genes, well complementary overexpression were generated, respectively. results showed was highly conserved pathovars positively regulated growth rate, pathogenicity, resistance osmotic oxidative stress QSY6. While did not affect motility exopolysaccharide production abundant expression enhanced swimming ability QSY6 reduced extracellular polysaccharides. Furthermore, regulates number factors, including Hrp system, type VI secretion flagellar synthesis. Specifically, induced hrpL hrpRS vivo, repressed transcription tssC vitro, while promoting hrpS fliC tssJ . This study contributes better understanding mechanisms

Language: Английский

Citations

1
Isolation, Identification, and Biocontrol Mechanisms of Endophytic Burkholderia arboris DHR18 from Rubber Tree against Red Root Rot Disease DOI Creative Commons

Xiangjia Meng,

Youhong Luo,

Xinyang Zhao

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(9), P. 1793 - 1793

Published: Aug. 29, 2024

Red root rot disease is a devastating fungal of rubber trees caused by Ganoderma pseudoferreum (Wakef). Biocontrols using beneficial microorganisms are safe and sustainable. We isolated DHR18 endophytic bacterium from healthy tree to obtain new efficient antagonistic for red affecting evaluated the mechanism action involved double culture assay, genome annotation analysis, ethyl acetate extraction method. The results revealed that strain inhibits G. growth has broad-spectrum antifungal activity secreting cell wall hydrolases (proteases chitinases), indole-3-acetic acid, siderophores. Furthermore, it fixes nitrogen in biofilm formation phosphate solubilisation, improving resistance growth. showed substances secreted mainly lipopeptides. Simultaneously, enhanced increasing activities defence enzymes superoxide dismutase, phenylalanine ammonia lyase, peroxidase, catalase, polyphenol oxidase. indicate B. arboris biocontrol potential could be used as candidate control trees.

Language: Английский

Citations

1
Complete Genome Resource of a Fungal Antagonistic Strain: Pseudomonas tohonis DOI Creative Commons
Wanzhen Luo, He Zhu,

Hong‐Yue Qi

et al.

PhytoFrontiers™, Journal Year: 2024, Volume and Issue: 4(3), P. 453 - 458

Published: Jan. 13, 2024

KRS022 was isolated from the rhizosphere soil of a healthy plant in pathogen-infested cotton field Xinjiang, China. Previous studies have demonstrated that possesses ability to trigger immune responses, inhibit growth various pathogenic fungi, including Verticillium dahliae, and promote growth. The antagonistic properties primarily stem its bioactive metabolites. In this study, high-quality genome obtained. consists chromosome 6,369,026 bp with 67.09% GC content 5,830 open reading frames, which 5,186 genes were annotated, 119 ncRNA genes. Additionally, 11 gene clusters secondary metabolites biosynthesis predicted, involved polyketide synthase, arylpolyene, NAGGN, beta-lactone, ranthipeptide, PiPP-like, redox cofactor, non-ribosomal peptide-synthetase clusters. It could be useful explore biocontrol potential P. tohonis, implying promising application biological control. [Formula: see text] Copyright © 2024 Author(s). This is an access article distributed under CC BY-NC-ND 4.0 International license .

Language: Английский

Citations

0
AlgU mediates hyperosmotic tolerance in Pseudomonas protegens SN15-2 by regulating membrane stability, ROS scavenging, and osmolyte synthesis DOI
Jian Wang, Yaping Wang,

Shouquan Lu

et al.

Applied and Environmental Microbiology, Journal Year: 2024, Volume and Issue: 90(8)

Published: Aug. 21, 2024

can serve as an agricultural biocontrol agent.

Language: Английский

Citations

0
Topical treatment of adult house flies, Musca domestica L. (Diptera: Muscidae), with Beauveria bassiana in combination with three entomopathogenic bacteria DOI Open Access
Dana M. Johnson, Emma N.I. Weeks, Eric D. LoVullo

et al.

Florida Entomologist, Journal Year: 2024, Volume and Issue: 107(1)

Published: Jan. 1, 2024

Abstract Biological control of house flies, Musca domestica L. (Diptera: Muscidae) has the potential to improve efficacy and sustainability integrated pest management. In a previous study, we demonstrated that three bacteria species ( Serratia marcescens Bizio [Enterobacteriales: Enterobacteriaceae], Photorhabdus temperata Fischer-Le Saux et al. Pseudomonas protegens Ramette [Pseudomonadales: Pseudomonadaceae]) are extremely virulent, inducing rapid morbidity high mortality in adult flies when injected into hemocoel but show little effect applied topically. Here tested hypothesis topical application these combination with entompathogenic fungus Beauveria bassiana (Bals. Criv.) Vuill. (Hypocreales: Cordycipitaceae) would result an increased if fungal penetration fly cuticle allowed for delivery hemocoel. Bacteria B. were either by at same time or sequential two pathogens 48 h apart. Results indicated evidence synergy between any bacterial pathogens. However, P. caused early (<50 % 3 days) alone . A modified disc diffusion assay had inhibitory effects on vegetative growth suggesting release antifungal molecules bacteria. sufficiently encouraging warrant further investigation this its associated toxins as biological tools.

Language: Английский

Citations

0