Identification of a transcription factor AoMsn2 of the Hog1 signaling pathway contributes to fungal growth, development and pathogenicity in Arthrobotrys oligospora

Journal of Advanced Research, Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 1, 2024

Arthrobotrys oligospora has been utilized as a model strain to study the interaction between fungi and nematodes owing its ability capture by developing specialized traps. A previous showed that high-osmolarity glycerol (Hog1) signaling regulates osmoregulation nematocidal activity of A. oligospora. However, function downstream transcription factors Hog1 in nematode-trapping (NT) remains unclear. This aimed investigate functions potential regulatory network AoMsn2, factor pathway The AoMsn2 was characterized using targeted gene deletion, phenotypic experiments, real-time quantitative PCR, RNA sequencing, untargeted metabolomics, yeast two-hybrid analysis. Loss Aomsn2 significantly enlarged swollen hyphae, with an increase septa significant decrease nuclei. In particular, spore yield, germination rate, traps, nematode predation efficiency were remarkably decreased mutants. Phenotypic transcriptomic analyses revealed is essential for fatty acid metabolism autophagic pathways. Additionally, metabolomic analysis identified important modulation secondary metabolites. Furtherly, we analyzed protein based on Kyoto Encyclopedia Genes Genomes map online website STRING. Finally, six putative proteins Y2H Our reveals plays crucial roles growth, conidiation, trap development, metabolism, well establishes broad basis understanding mechanisms morphogenesis environmental adaptation NT fungi.

Language: Английский

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The Arf-GAPs, AoAge1 and AoAge2, regulate diverse cellular processes, conidiation, trap formation, and secondary metabolism in Arthrobotrys oligospora

Microbiological Research, Journal Year: 2024, Volume and Issue: 285, P. 127779 - 127779

Published: May 24, 2024

Language: Английский

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The Hog1-Nmd5 signaling pathway regulates asexual development, lipid metabolism, stress response, trap morphogenesis, and secondary metabolism of Arthrobotrys oligospora

Virulence, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 19, 2025

The high-osmolarity glycerol (HOG) signalling pathway, comprising Ste11/Ssk2/Ssk22 (MAPKKK), Pbs2 (MAPKK), and Hog1 (MAPK), is an important conserved pathway in fungi. However, the functions downstream regulatory factors of nematode-trapping (NT) fungi remain poorly understood. Here, three proteins (AoNmd5, AoPyp1, AoPtp) interacting with were screened a representative NT fungus Arthrobotrys oligospora using yeast screening library verified two-hybrid (Y2H) assay. function AoNmd5 was furtherly characterized by phenotypic comparison, staining technique, multi-omics analyses. essential for vegetative growth, conidial development, trap morphogenesis, nematode predation ability. In addition, played crucial roles endocytosis, lipid metabolism, reactive oxygen species, stress response, autophagy, other metabolic processes. Furthermore, we constructed interaction network based on transcriptomic analysis Y2H, revealing its significant role respiratory chain redox processes as well small GTPase Ran1, which mediates nucleocytoplasmic shuttling. These findings suggest that Hog1-Nmd5 has pleiotropic A. oligospora. This study deepens our understanding HOG importins

Language: Английский

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Characterizing the Role of AosfgA and AofluG in Mycelial and Conidial Development in Arthrobotrys oligospora and Their Role in Secondary Metabolism

Microorganisms, Journal Year: 2024, Volume and Issue: 12(3), P. 615 - 615

Published: March 19, 2024

Arthrobotrys oligospora, a widespread nematode-trapping fungus which can produce conidia for asexual reproduction and form trapping devices (traps) to catch nematodes. However, little is known about the sporulation mechanism of A. oligospora. This research characterized functions regulatory roles upstream spore-producing genes, AosfgA AofluG, in Our analysis showed that AofluG interacted with each other. Meanwhile, gene was downregulated ΔAosfgA mutant strain, indicating positively regulates AofluG. Loss genes led shorter hyphae more septa, strain responded heat chemical stresses. Surprisingly, number nuclei increased mycelia but reduced ΔAofluG mutants. In addition, after nematode induction, volume vacuoles were remarkably strains. The abundance metabolites markedly decreased Collectively, play critical mycelial development, they are also involved vacuole assembly, stress response, secondary metabolism. study provides distinct insights into fungi.

Language: Английский

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AoPrdx2 Regulates Oxidative Stress, Reactive Oxygen Species, Trap Formation, and Secondary Metabolism in Arthrobotrys oligospora

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(2), P. 110 - 110

Published: Jan. 28, 2024

Prdx2 is a peroxiredoxin (Prx) family protein that protects cells from attack via reactive oxygen species (ROS), and it has an important role in improving the resistance scavenging capacity of ROS fungi. Arthrobotrys oligospora widespread nematode-trapping fungus can produce three-dimensional nets to capture kill nematodes. In this study, AoPrdx2, homologous Prx5, was investigated A. gene disruption, phenotypic analysis, metabolomics. The deletion Aoprdx2 resulted increase number mycelial septa reduction nuclei spore yield. Meanwhile, absence increased sensitivity oxidative stresses, whereas ∆Aoprdx2 mutant strain higher levels than wild-type (WT) strain. particular, inactivation severely influenced trap formation pathogenicity; traps produced by remarkably reduced rings less WT addition, abundance metabolites significantly downregulated compared with These results indicate AoPrdx2 plays indispensable ROS, morphogenesis, secondary metabolism.

Language: Английский

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The Cryptochrome CryA Regulates Lipid Droplet Accumulation, Conidiation, and Trap Formation via Responses to Light in Arthrobotrys oligospora

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(9), P. 626 - 626

Published: Sept. 1, 2024

Light is a key environmental factor affecting conidiation in filamentous fungi. The cryptochrome/photolyase CryA, blue-light receptor, involved fungal development. In the present study, homologous CryA (AoCryA) was identified from widely occurring nematode-trapping (NT) fungus Arthrobotrys oligospora, and its roles mycelial growth development of A. oligospora were characterized using gene knockout, phenotypic comparison, staining technique, metabolome analysis. inactivation AocryA caused substantial decrease spore yields dark conditions but did not affect wild-type (WT) ∆AocryA mutant strains light conditions. Corresponding to production, transcription sporulation-related genes also significantly downregulated Contrarily, mutants showed increase trap formation conditions, while production abilities WT decreased addition, lipid droplet accumulation increased an tolerance sorbitol, contributed synthesis carotenoids. Finally, AoCryA found secondary metabolic processes. These results reveal, for first time, function cryptochrome NT

Language: Английский

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Functional Characterization of Ao4g24: An Uncharacterized Gene Involved in Conidiation, Trap Formation, Stress Response, and Secondary Metabolism in Arthrobotrys oligospora

Microorganisms, Journal Year: 2024, Volume and Issue: 12(8), P. 1532 - 1532

Published: July 26, 2024

is a typical nematode-trapping (NT) fungus, which can secrete food cues to lure, capture, and digest nematodes by triggering the production of adhesive networks (traps). Based on genomic proteomic analyses, multiple pathogenic genes proteins involved in trap formation have been characterized; however, there are numerous uncharacterized that play important roles formation. The functional studies these unknown helpful systematically elucidating complex interactions between

Language: Английский

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AoRan1 Is Involved in Regulating Conidiation, Stress Resistance, Secondary Metabolism, and Pathogenicity in Arthrobotrys oligospora

Microorganisms, Journal Year: 2024, Volume and Issue: 12(9), P. 1853 - 1853

Published: Sept. 6, 2024

is a representative nematode-trapping (NT) fungus that able to capture, kill, and digest nematodes by producing specialized three-dimensional networks (traps) under nutrient-deprived conditions. Ran1 serine/threonine protein kinase can act as negative regulator of sexual conjugation meiosis. However, the specific role remains largely unknown in NT fungi. Here, we identified AoRan1 (AOL_s00004g277) via gene disruption, phenotypic analysis, metabolomic analysis. Our findings reveal

Language: Английский

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Genomic and Transcriptomic Analyses Revealed DdSTE2 Play a Role in Constricting Ring Formation in the Nematode-Trapping Fungi Drechslerella dactyloides

Microorganisms, Journal Year: 2024, Volume and Issue: 12(11), P. 2190 - 2190

Published: Oct. 30, 2024

The carnivorous fungus

Language: Английский

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