Hesperetin Alleviated Experimental Colitis via Regulating Ferroptosis and Gut Microbiota

Nutrients, Journal Year: 2024, Volume and Issue: 16(14), P. 2343 - 2343

Published: July 19, 2024

Hesperetin (HT) is a type of citrus flavonoid with various pharmacological activities, including anti-tumor, anti-inflammation, antioxidant, and neuroprotective properties. However, the role mechanism HT in ulcerative colitis (UC) have been rarely studied. Our study aimed to uncover beneficial effects its detailed UC. Experimental was induced by 2.5% dextran sodium sulfate (DSS) for seven days. ameliorated DSS-induced mice, showing marked improvement weight loss, colon length, colonic pathological severity, levels TNFα IL6 serum. A combination informatics, network pharmacology, molecular docking identified eight key targets multi-pathways influenced As highlight, experimental validation demonstrated that PTGS2, marker ferroptosis, along other indicators ferroptosis (such as ACSL4, Gpx4, lipid peroxidation), were regulated vivo vitro. Additionally, supplement increased diversity gut microbiota, decreased relative abundance Proteobacteria Gammaproteobacteria, restored bacteria (Lachnospiraceae_NK4A136_group Prevotellaceae_UCG-001). In conclusion, an effective nutritional against suppressing modulating microbiota.

Language: Английский

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Gut Microbiota Profile Changes in Patients with Inflammatory Bowel Disease and Non-Alcoholic Fatty Liver Disease: A Metagenomic Study

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(10), P. 5453 - 5453

Published: May 17, 2024

Gut microbiota imbalances have a significant role in the pathogenesis of Inflammatory Bowel Disease (IBD) and Non-Alcoholic Fatty Liver (NAFLD). Herein, we compared gut microbial composition patients diagnosed with either IBD or NAFLD combination both. Seventy-four participants were stratified into four groups: IBD-NAFLD, IBD-only, NAFLD-only patients, healthy controls (CTRLs). The 16S rRNA was sequenced by Next-Generation Sequencing. Bioinformatics statistical analysis performed. Bacterial α-diversity showed lower value when IBD-only group to other groups particularly against IBD-NAFLD group. β-diversity also difference among groups. higher Bacteroidetes/Firmicutes ratio found only comparing CTRLs. Comparing group, decrease differential abundance Subdoligranulum, Parabacteroides, Fusicatenibacter found. groups, there Alistipes, Odoribacter, Sutterella, Lachnospira. An inverse relationship comparison between shown. For first time, singularity has been shown, implying potential signature mainly influenced inflammation.

Language: Английский

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Prediction of vedolizumab efficacy in ulcerative colitis: a nomogram incorporating pathological feature and serological marker

Clinical and Experimental Medicine, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 2, 2025

Vedolizumab (VDZ) is a humanized, gut-selective biologic used in the treatment of ulcerative colitis (UC). However, data on predictive factors for response are limited. This study aims to develop nomogram predict VDZ responsiveness UC. We retrospectively collected clinical from patients with moderate-to-severe active UC who received induction therapy at Chongqing General Hospital December 2020 March 2024. Full-slide images colon biopsies prior were analyzed quantify mean mucosal eosinophil density (MMED). Based 14-week post-treatment, categorized into responsive and non-responsive groups. In total, 84 analyzed, 58 responding 26 not responding. Significant differences observed pathological indices, MMED showing statistically significant difference between groups (p < 0.001). Serum biomarkers, including C-reactive protein (CRP), also showed (P = 0.015), as did CRP/albumin (CRP/ALB) ratio 0.018). Additionally, UCEIS scores differed significantly 0.025). Independent risk identified through multivariate logistic regression analysis establish model, presented nomogram. The area under curve (AUC) combined CRP model was 0.867 (95% CI: 0.781–0.953, p 0.001), indicating high accuracy predicting efficacy. These easily accessible even primary healthcare settings, allowing our support improved decisions patients.

Language: Английский

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Marine-Derived Enterococcus faecalis HY0110 as a Next-Generation Functional Food Probiotic: Comprehensive In Vitro and In Vivo Bioactivity Evaluation and Synergistic Fermentation of Periplaneta americana Extract Powder

Foods, Journal Year: 2025, Volume and Issue: 14(7), P. 1181 - 1181

Published: March 28, 2025

Addressing the escalating global burdens of inflammatory bowel disease and antimicrobial resistance demanded innovative food-based approaches to fortify gut health suppress pathogens. We introduced a novel edible probiotic, Enterococcus faecalis HY0110, isolated from marine Thunnus thynnus. Through comprehensive in vitro, vivo, metabolomic analyses, we demonstrated its superior antibacterial effects compared Lactobacillus rhamnosus GG, along with significantly enhanced antioxidant free-radical scavenging capacities. Notably, elevated acetic acid production strongly correlated efficacy (R ≥ 0.999). HY0110 also exerted antiproliferative on HT-29 colorectal cancer cells by attenuating β-catenin BCL-2 expression while upregulating pro-apoptotic markers P62 c-PARP. In DSS-induced colitis model, alleviated inflammation, restored microbial homeostasis, deterministic processes community assembly dynamics. Furthermore, fermenting Periplaneta americana powder triggered extensive metabolic remodeling, notably 668.73-fold rise astragaloside A, plus increases L-Leucyl-L-Alanine, S-lactoylglutathione, 16,16-dimethyl prostaglandin A1. These shifts diminished harmful components amplified essential amino acids peptides bolster immune modulation, redox balance, anti-inflammatory responses. This work established transformative paradigm for utilizing probiotics entomological substrates functional foods, presenting strategic pathways precision nutrition management.

Language: Английский

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Inorganic dietary nanoparticles in intestinal barrier function of inflammatory bowel disease: allies or adversaries?

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 9, 2025

Inorganic dietary nanoparticles (IDNPs) are frequently utilized as food additives and in packaging, resulting their exposure becoming a substantial yet often overlooked concern for patients with inflammatory bowel disease (IBD). Considering that impaired intestinal barrier function plays central role the pathogenesis of IBD, this review concentrates on roles mechanisms IDNPs (physical, chemical, biological, immune barriers) IBD patients. Previous studies have shown different types varying effects animals diverse states. In context, factors such source, size, shape, dosage, duration action nanoparticles, well species, gender, habits, age animals, significantly influence research outcomes. Future should undertake more comprehensive explorations into sources properties

Language: Английский

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Bisphenol A Exposure Induces Small Intestine Damage Through Oxidative Stress, Inflammation, and Microbiota Alteration in Rats

Toxics, Journal Year: 2025, Volume and Issue: 13(5), P. 331 - 331

Published: April 23, 2025

Bisphenol A (BPA), a widespread environmental contaminant used in plastics and resins, poses significant health risks due to its endocrine-disrupting properties potential for inducing intestinal toxicity. This study explored the toxicological effects of BPA on small intestine rats, focusing duodenum, jejunum, ileum. Histopathological evaluation revealed that duodenum experienced most severe structural damage, including villous atrophy, epithelial shedding, mitochondrial degeneration. exposure disrupted oxidative stress homeostasis by reducing superoxide dismutase activity increasing malondialdehyde levels, along with upregulating antioxidant-related genes like GPX2 HO-1 upregulated, indicating lipid peroxidation damage. Inflammatory markers such as IL-1 NFκB were significantly highlighting an active inflammatory response cell apoptosis. also altered metabolism, increased expression lipogenic SREBP-1c FAS, metabolic dysregulation. Fecal microbiota analysis reduced α-diversity, enrichment pathogenic taxa Escherichia-Shigella, depletion beneficial genera Lachnospiraceae NK4A136 group, exacerbating gut inflammation barrier dysfunction. These findings suggest BPA-induced damage is driven stress, inflammation, dysbiosis, jejunum being more vulnerable segments.

Language: Английский

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Fecal microbiota and metabolites in the pathogenesis and precision medicine for inflammatory bowel disease

Precision Clinical Medicine, Journal Year: 2024, Volume and Issue: 7(3)

Published: July 24, 2024

Abstract Inflammatory bowel disease (IBD) is a chronic inflammatory disorder of the gastrointestinal tract, and its pathogenesis believed to be associated with an imbalance between commensal organisms intestinal immune system. This significantly influenced by microbiota metabolites plays critical role in maintaining mucosal homeostasis. However, disturbances cause dysregulated responses consequently induce inflammation. Recent studies have illustrated roles IBD underscored potential precision diagnosis therapy. work summarises recent progress this field particularly focuses on application diagnosis, prognosis assessment, treatment effectiveness evaluation, therapeutic management IBD.

Language: Английский

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Abdominal Pain in Inflammatory Bowel Disease-Epidemiology, Pathophysiology, and Management: A Narrative Review

Pain and Therapy, Journal Year: 2024, Volume and Issue: 13(6), P. 1447 - 1469

Published: Oct. 28, 2024

Abdominal pain is a major symptom of inflammatory bowel disease (IBD), including Crohn's and ulcerative colitis, has significant impact on patients' quality life. Given the evolving understanding IBD pathology management strategies, there an urgent need to review recent research findings. In this review, we have analyzed epidemiology, pathophysiology, abdominal in over past decade. We draw current literature highlight emerging trends, challenges, advances field. By synthesizing key findings, provides insights into complex interplay between pain, progression, therapeutic interventions for IBD.

Language: Английский

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Nuclear Magnetic Resonance (NMR) Metabolomics: Current Applications in Equine Health Assessment

Metabolites, Journal Year: 2024, Volume and Issue: 14(5), P. 269 - 269

Published: May 7, 2024

Metabolomics can allow for the comprehensive identification of metabolites within biological systems, at given time points, in physiological and pathological conditions. In last few years, metabolomic analysis has gained popularity both human veterinary medicine, showing great potential novel applications clinical activity. The aim applying metabolomics practice is understanding mechanisms underlying conditions influence certain stimuli (i.e., drugs, nutrition, exercise) on body attempt identifying biomarkers that help diagnosis diseases. Proton Nuclear Magnetic Resonance spectroscopy (

Language: Английский

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Rolul microbiotei intestinale în sindromul de colon iritabil

˜The œRomanian Journal of Nutrition., Journal Year: 2024, Volume and Issue: 2(2), P. 14 - 14

Published: Jan. 1, 2024

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