Immunomodulatory Effects of SPHK1 and Its Interaction with TFAP2A in Yellow Drum (Nibea albiflora) DOI Open Access
Yu Cui,

Shuai Luo,

Baolan Wu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13641 - 13641

Published: Dec. 20, 2024

Sphingosine kinases (SPHKs) are essential enzymes that catalyze the phosphorylation of sphingosine to produce sphingosine-1-phosphate (S1P), which plays pivotal roles in inflammation and immune regulation. In this study, genome-wide association analysis (GWAS) identified Ydsphk1 gene as closely associated with resistance yellow drum (Nibea albiflora) Vibrio harveyi. Structural prediction showed YDSPHK1 contains a typical diacylglycerol kinase catalytic (DAGKc) domain (154–291 aa). By constructing transfecting expression plasmids into kidney cells, we found is localized cytoplasm. Subsequent RNA-Seq an overexpression plasmid 25 differentially expressed genes (DEGs), including 13 upregulated 12 downregulated. Notably, nsun5 hsp90aa1 were significantly upregulated, while Nfkbia hmox1 Promoter indicated core regulatory regions located between −1931~−1679 bp −419~+92 bp, two predicted TFAP2A binding sites region. Further studies demonstrated varying concentrations reduced promoter activity. These findings underscore role regulating responses drum, particularly through its impact on key immune-related pathways such NF-κB signaling ferroptosis. The identification mediator regulation provides valuable insights molecular mechanisms defense highlights potential target for enhancing pathogen aquaculture practices. This study lays strong foundation future research aimed at developing innovative strategies disease management species.

Language: Английский

Mitophagy and Ferroptosis in Sepsis-Induced ALI/ARDS: Molecular Mechanisms, Interactions and Therapeutic Prospects of Medicinal Plants DOI Creative Commons
Huixin Cheng, Xuehan Wang,

Juyi Yao

et al.

Journal of Inflammation Research, Journal Year: 2024, Volume and Issue: Volume 17, P. 7819 - 7835

Published: Oct. 1, 2024

Sepsis is a common critical illness characterized by high mortality rates and significant disease burden. In the context of sepsis-induced organ dysfunction, lungs are among initial organs affected, which may progress to acute lung injury (ALI) respiratory distress syndrome (ARDS). Recent studies have highlighted crucial roles mitophagy ferroptosis in development progression ALI/ARDS. Identifying key convergence points these processes provide valuable insights for treatment this condition. recent years, certain herbs their bioactive compounds demonstrated unique benefits managing ALI/ARDS modulating or ferroptosis. This review summary mechanisms ferroptosis, explores interactions, emphasizes regulatory Additionally, it offers novel perspective on strategies summarizing various relevant

Language: Английский

Citations

4
Isobavachalcone confers protection against Cryptococcus neoformans-induced ferroptosis in Caenorhabditis elegans via lifespan extension and GSH-GPX-1 axis modulation DOI
Weidong Qian, Jiaxing Lu, Ting Wang

et al.

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: unknown, P. 137969 - 137969

Published: March 1, 2025

Language: Английский

Citations

0
Integration of Transcriptomic and Single-Cell Data to Uncover Senescence- and Ferroptosis-Associated Biomarkers in Sepsis DOI Creative Commons
Xiangqian Zhang, Yiran Zhou, Hang Li

et al.

Biomedicines, Journal Year: 2025, Volume and Issue: 13(4), P. 942 - 942

Published: April 11, 2025

Background: Sepsis is a life-threatening condition characterized by organ dysfunction due to an imbalanced immune response infection, with high mortality. Ferroptosis, iron-dependent cell death process, and cellular senescence, which exacerbates inflammation, have recently been implicated in sepsis pathophysiology. Methods: Weighted gene co-expression network analysis (WGCNA) was used identify ferroptosis- senescence-related modules sepsis. Differentially expressed genes (DEGs) were analyzed using public datasets (GSE57065, GSE65682, GSE26378). Receiver operating characteristic (ROC) performed evaluate their diagnostic potential, while single-cell RNA sequencing (scRNA-seq) assess immune-cell-specific expression. Molecular docking conducted predict drug interactions key proteins. Results: Five (CD82, MAPK14, NEDD4, TXN, WIPI1) significantly upregulated patients highly correlated infiltration. MAPK14 TXN exhibited strong potential (AUC = 0.983, 0.978). suggested therapeutic diclofenac, flurbiprofen, N-acetyl-L-cysteine. Conclusions: This study highlights ferroptosis senescence as critical mechanisms identifies promising biomarkers for diagnosis targeted therapy. Future studies should focus on clinical validation precision medicine applications.

Language: Английский

Citations

0
The Role of Ferroptosis and Cuproptosis in Tuberculosis Pathogenesis: Implications for Therapeutic Strategies DOI Creative Commons
John Dawi,

Stephen Affa,

Kevin Kafaja

et al.

Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(2), P. 99 - 99

Published: Feb. 5, 2025

Tuberculosis (TB) caused by Mycobacterium tuberculosis (M.tb) remains a global health crisis, with over 10 million people affected annually. Despite advancements in treatment, M.tb has developed mechanisms to evade host immune responses, complicating efforts eradicate the disease. Two emerging cell death pathways, ferroptosis and cuproptosis, have been linked TB pathogenesis. Ferroptosis, an iron-dependent form of death, is driven lipid peroxidation reactive oxygen species (ROS) accumulation. This process can limit replication depleting intracellular iron inducing macrophage necrosis. However, excessive may lead tissue damage aid bacterial dissemination. Cuproptosis, triggered copper accumulation, disrupts mitochondrial metabolism, leading protein aggregation death. exploits both metabolism survive within macrophages, manipulating these processes resist oxidative stress responses. review examines roles cuproptosis TB, discussing how manipulates pathways for survival. While therapeutic strategies targeting processes, such as inducers (Erastin, RSL3) inhibitors (Ferrostatin-1) ionophores (Disulfiram, Elesclomol) chelators, show promise, limited understanding potential off-target effects significant challenge. Further exploration provide insights into development targeted therapies aimed at controlling infection while minimizing damage. By elucidating complex interactions between ferroptosis, future could better address resistance improve clinical outcomes.

Language: Английский

Citations

0
Physical Fields Reverse FeSO4-Induced VBNC State in Listeria Monocytogenes and Facilitate Ferroptosis DOI
Xiaolin Zhu, Yunhong Wang, Shurui Peng

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Physical fields reverse FeSO4-induced VBNC state in Listeria monocytogenes and facilitate ferroptosis DOI
Xiaolin Zhu, Yunhong Wang, Shurui Peng

et al.

Food Microbiology, Journal Year: 2025, Volume and Issue: unknown, P. 104796 - 104796

Published: April 1, 2025

Language: Английский

Citations

0
Experimental and Computational Study of Injectable Iron(III)/Ultrashort Peptide Hydrogels: A Candidate for Ferroptosis‐Induced Treatment of Bacterial Infections DOI Creative Commons
Capucine Loth, Florent Barbault,

Christelle Guégan

et al.

Small Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

Injectable hydrogels are promising candidates as local drug delivery platforms for the treatment of infected wounds. Self‐assembled small peptide interest due to their high biocompatibility, degradability, and ease synthesis. This study describes formation an injectable hydrogel based on self‐assembly Fmoc‐FFpY (Fmoc: fluorenylmethoxycarbonyl, F: phenylalanine, pY: tyrosine phosphate) triggered by electrostatic interactions in presence Fe 3+ ions. Stabilized H bonding π–π stacking, exhibit mechanical stiffness with a G ′ (storage modulus) ≈8000 Pa self‐recovery up ≈100 Pa. Peptide yields β‐sheets twisted into fibrillar helices 12 nm diameter pitch. Molecular dynamics simulations confirm 1) aggregation adopted secondary structure show that 2) aggregated Fmoc‐FFpY/Fe disrupts bacterial membrane Staphylococcus aureus Pseudomonas aeruginosa , favoring passive entry pathogen. In full agreement simulations, antibacterial activity against both bacteria, likely increased cell, resulting enhanced production reactive oxygen species. work paves way ferroptosis‐inducing infections using ultrashort peptides.

Language: Английский

Citations

0
Inhibition of calpain reduces oxidative stress and attenuates pyroptosis and ferroptosis in Clostridium perfringens Beta-1 toxin-induced macrophages DOI

Siyu Zhang,

Dong Wang,

Yawen Ding

et al.

Microbiological Research, Journal Year: 2024, Volume and Issue: 289, P. 127916 - 127916

Published: Sept. 27, 2024

Language: Английский

Citations

1
Immunomodulatory Effects of SPHK1 and Its Interaction with TFAP2A in Yellow Drum (Nibea albiflora) DOI Open Access
Yu Cui,

Shuai Luo,

Baolan Wu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13641 - 13641

Published: Dec. 20, 2024

Sphingosine kinases (SPHKs) are essential enzymes that catalyze the phosphorylation of sphingosine to produce sphingosine-1-phosphate (S1P), which plays pivotal roles in inflammation and immune regulation. In this study, genome-wide association analysis (GWAS) identified Ydsphk1 gene as closely associated with resistance yellow drum (Nibea albiflora) Vibrio harveyi. Structural prediction showed YDSPHK1 contains a typical diacylglycerol kinase catalytic (DAGKc) domain (154–291 aa). By constructing transfecting expression plasmids into kidney cells, we found is localized cytoplasm. Subsequent RNA-Seq an overexpression plasmid 25 differentially expressed genes (DEGs), including 13 upregulated 12 downregulated. Notably, nsun5 hsp90aa1 were significantly upregulated, while Nfkbia hmox1 Promoter indicated core regulatory regions located between −1931~−1679 bp −419~+92 bp, two predicted TFAP2A binding sites region. Further studies demonstrated varying concentrations reduced promoter activity. These findings underscore role regulating responses drum, particularly through its impact on key immune-related pathways such NF-κB signaling ferroptosis. The identification mediator regulation provides valuable insights molecular mechanisms defense highlights potential target for enhancing pathogen aquaculture practices. This study lays strong foundation future research aimed at developing innovative strategies disease management species.

Language: Английский

Citations

0