Sterol Regulatory Element-Binding Protein Sre1 Mediates the Development and Pathogenicity of the Grey Mould Fungus Botrytis cinerea

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 1365 - 1365

Published: Feb. 6, 2025

The grey mould fungus Botrytis cinerea is a dangerous plant pathogen responsible for substantial agricultural losses worldwide. pathogenic mechanisms still have many unclear aspects, and numerous new genes remain to be identified. Here, we show that the sterol regulatory element-binding protein Sre1 plays an important role in development pathogenicity of B. cinerea. We identified homologue gene SRE1 genome utilized reverse genetics approach create knockout mutant Δsre1. Our results demonstrate essential conidiation, as Δsre1 produced only 3% conidia compared wild-type strain. Conversely, exhibited increased sclerotium production, indicating negative formation. Furthermore, ergosterol biosynthesis was significantly reduced mutant, correlating with sensitivity low-oxygen conditions. Pathogenicity assays revealed had virulence, although it maintained normal infection cushion formation penetration capabilities. Additionally, found crucial hypoxia adaptation, showed abnormal germination growth under These findings suggest mediates by regulating lipid homeostasis facilitating adaptation host tissue environments.

Language: Английский

Transcription Factors in Biocontrol Fungi

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(3), P. 223 - 223

Published: March 15, 2025

Transcription factors are extensively found in fungi and involved the regulation of multiple biological processes, including growth, development, conidiation, morphology, stresses tolerance, virulence, as well production secondary metabolites. Biocontrol is a complex process through which several biocontrol behaviors, such secretion cell wall-degrading enzymes metabolites, regulated by transcription factors. To date, biocontrol-related have been reported fungi, Beauveria bassiana, Clonostachys rosea, Coniothyrium minitans, different species genera Metarhizium, Trichoderma, Arthrobotrys. However, comprehensive reviews summarizing analyzing with potential these scarce. This review begins giving basic overview their functions. Then, role discussed. Lastly, possible approaches for further work on suggested. provides basis elaborating molecular mechanisms context biocontrol.

Language: Английский