Ferroptosis-Related Genes as Molecular Markers in Bovine Mammary Epithelial Cells Challenged with Staphylococcus aureus

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2506 - 2506

Published: March 11, 2025

Staphylococcus aureus-induced mastitis is a significant cause of economic losses in the dairy industry, yet its molecular mechanisms remain poorly defined. Although ferroptosis, regulated cell death process, associated with inflammatory diseases, role bovine unknown. In this study, 11 S. aureus strains were isolated from milk samples obtained cows clinical or subclinical mastitis. Transcriptome analysis Mac-T cells challenged identified differentially expressed genes (DEGs). Enrichment revealed associations between DEG clusters and traits related to KEGG pathway enrichment Toll-like receptor, TNF signaling as significantly enriched pathways. Weighted gene co-expression network (WGCNA) further prioritized ferroptosis-related (HMOX1, SLC11A2, STEAP3, SAT1, VDAC2) involved iron metabolism. Notably, expression levels HMOX1 SAT1 increased aureus-challenged cells, upregulation was consistent trends observed transcriptome data mother–daughter pairs caused by infection. Furthermore, Ferrostatin-1 treatment reduced confirming involvement ferroptosis process. This study reveals that plays key highlights potential target for breeding strategies aimed at improving resistance.

Language: Английский

Host Specificity of Snodgrassella in Eastern and Western Honeybees and Its Effects on Naturally Occurring Deformed Wing Virus Titers

Insects, Journal Year: 2025, Volume and Issue: 16(5), P. 478 - 478

Published: May 1, 2025

Honeybee gut microbiota undergoes long-term adaptive evolution with the host, resulting in host-specific genomic and functional characteristics. However, specific role of host-specialized strains shaping host physiological functions remains understudied. This study investigates specificity core symbiont Snodgrassella A. cerana mellifera, exploring its effects on immune response natural virus suppression through analysis colonization experiments. Genomic revealed that strain from exhibited a larger genome greater gene content compared to cerana. Competitive experiments showed although different origins had similar efficiency displayed clear home-field advantage competitive process. Moreover, Inoculation mellifera native significantly reduced Deformed Wing Virus (DWV) titers, whereas non-native no effect. In contrast, neither altered DWV or Sacbrood (SBV) levels Immune only upregulated defensin 2 while significant changes occurred either strain. These results suggest exhibits at level, influencing both suppression, showing efficacy these roles, especially mellifera.

Language: Английский