Characterization and genomic analysis of Sharanji: a jumbo bacteriophage of Escherichia coli DOI Creative Commons
Sharayu Magar, Sivaraj Barath, Debmitra Sen

et al.

Virology Journal, Journal Year: 2025, Volume and Issue: 22(1)

Published: March 10, 2025

Bacteriophages are the most genetically diverse biological entities in nature. Our current understanding of phage biology primarily stems from studies on a limited number model bacteriophages. Jumbo phages, characterized by their exceptionally large genomes, less frequently isolated and studied. Some jumbo phages exhibit remarkable genetic diversity, unique infection mechanisms, therapeutic potential. In this study, we describe isolation Sharanji, novel Escherichia coli phage, chicken feces. The genome was sequenced analyzed extensively through gene annotation phylogenetic analysis. phenotypically electron microscopy, host range analysis, survival at different pH temperatures, one-step growth curve assay. Finally, Sharanji mediated E. is studied fluorescence to analyze its mechanism compared well-studied nucleus-forming phages. Whole sequencing reveals that has size 350,079 bp encompassing 593 ORFs. Genomic analysis indicates belongs Asteriusvirus genus related PBECO4 121Q. Phenotypic 245.3 nm, it narrow-spectrum infecting K12 strains, but not other bacteria including avian pathogenic coli. Infection using microscopy shows causes cell filamentation. Furthermore, intracellular nucleus-like structures were observed Sharanji-infected cells, contrast ΦKZ-like study reports characterization one Both genotypic phenotypic analyses suggest serves as system for studying phage-bacteria interactions, particularly within context non-nucleus-forming Further exploration holds promise uncovering new paradigms microbial viruses.

Language: Английский

Characterization and genomic analysis of Sharanji: a jumbo bacteriophage of Escherichia coli DOI Creative Commons
Sharayu Magar, Sivaraj Barath, Debmitra Sen

et al.

Virology Journal, Journal Year: 2025, Volume and Issue: 22(1)

Published: March 10, 2025

Bacteriophages are the most genetically diverse biological entities in nature. Our current understanding of phage biology primarily stems from studies on a limited number model bacteriophages. Jumbo phages, characterized by their exceptionally large genomes, less frequently isolated and studied. Some jumbo phages exhibit remarkable genetic diversity, unique infection mechanisms, therapeutic potential. In this study, we describe isolation Sharanji, novel Escherichia coli phage, chicken feces. The genome was sequenced analyzed extensively through gene annotation phylogenetic analysis. phenotypically electron microscopy, host range analysis, survival at different pH temperatures, one-step growth curve assay. Finally, Sharanji mediated E. is studied fluorescence to analyze its mechanism compared well-studied nucleus-forming phages. Whole sequencing reveals that has size 350,079 bp encompassing 593 ORFs. Genomic analysis indicates belongs Asteriusvirus genus related PBECO4 121Q. Phenotypic 245.3 nm, it narrow-spectrum infecting K12 strains, but not other bacteria including avian pathogenic coli. Infection using microscopy shows causes cell filamentation. Furthermore, intracellular nucleus-like structures were observed Sharanji-infected cells, contrast ΦKZ-like study reports characterization one Both genotypic phenotypic analyses suggest serves as system for studying phage-bacteria interactions, particularly within context non-nucleus-forming Further exploration holds promise uncovering new paradigms microbial viruses.

Language: Английский

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