A Comprehensive Review of Instrumentation and Applications in Post‐Column and In‐Source Derivatization for LC‐MS

Mass Spectrometry Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

ABSTRACT Liquid chromatography‐mass spectrometry (LC‐MS) has become an indispensable tool for elucidating molecular structures and quantifying diverse compounds within complex mixtures. Despite its versatility, it faces various challenges such as ion suppression, low sensitivity, analyte instability, matrix effects, which are being overcome by different kinds of offline online derivatization techniques to improve specificity reduce potential interferences. In this context, considerable advancements have been made in reviewing critically evaluating a wide range developed methods techniques; however, little attention given post‐column (PCD) LC‐MS. Therefore, comprehensive review highlights state‐of‐the‐art LC‐MS with specific focus on types chemical physical PCD, in‐source derivatization. It also examines the latest instrumentation developments, influencing factors, explores applications food, proteomics, biology, pharmaceuticals, environmental analysis from past four decades. Besides, role PCD along outlining advantages disadvantages. Furthermore, special emphasis is prospects insights developing more versatile LC‐PCD‐MS methodologies, address ongoing aim open new research avenues analysts.

Language: Английский

Analytical derivatization techniques

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024