An electrochemical biosensor using AuNPs-Ti3C2Tx and ARGET ATRP reactions as signal amplification strategies for ultra-sensitive detection of HER2 protein

Bioelectrochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 108970 - 108970

Published: March 1, 2025

Language: Английский

Entropy spontaneous ratiometric electrochemical aptasensor based on polymerization and AuNPs signal amplification for Acetamiprid residue analysis

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Acetamiprid (ACE), a next-generation chlorinated neonicotinoid insecticide, has been extensively employed for pest control. However, its excessive residues in food and the environment have raised significant concerns regarding human health. To address need simple, accurate, efficient ACE detection method, this study developed ratiometric electrochemical aptasensor utilizing dual signal amplification strategy involving atom transfer radical polymerization (ATRP) gold nanoparticles (AuNPs). Methylene blue (MB) served as internal reference signal, which was attached to aptamer-DNA₁ (dsDNA) through electrostatic adsorption intercalation. Ferrocenyl methyl methacrylate (FMMA) polymerized into long chains via ATRP, generating significantly amplified electrical compared that of monomeric FMMA. AuNPs, known their bioconjugability, were linked DNA₂ (AuNPs-DNA₂), enhancing loading capacity expanding ATRP reaction sites. In presence ACE, MB decreased while FMMA polymer increased, achieving with limit (LOD) 19.26 pg/mL. This not only enhances selectivity but also mitigates influence background currents, offering novel effective approach real-world sample analysis.

Language: Английский

Sensitive electrochemical detection of DR1 based on gold nanoparticle-modified MoS₂ and hyaluronic acid-based thionine

Sensors & Diagnostics, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

A novel dual-signal amplification electrochemical biosensor for sensitive detection of DR1 was constructed based on gold nanoparticle-modified molybdenum disulfide and hyaluronic acid-based thionine.

Language: Английский