Electrochemical assay for the quantification of anticancer drugs and their inhibition mechanism DOI Creative Commons
Ricardo J.B. Leote, Caroline G. Sanz, Victor C. Diculescu

et al.

Methods, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Overexpression of pyruvate kinase (PyK) is linked to many kinds malignant tumors, representing therefore one the most promising therapeutic targets for cancer treatment. Inhibition PyK slows down tumor growth or causes cell death, minimizing proliferation, and understanding inhibitor mechanism action can significantly improve therapy. The present work describes use an amperometric bienzymatic biosensor, based on oxidase (PyOx), in enzyme inhibition studies four inhibitors, CPG77675, Nilotinib, Ruxolitinib, Cerdulatinib. Their studied discussed detail, with a thorough evaluation their enzyme-inhibitor complex binding constants (Ki) concentration required 50% (IC50), employing standard procedure graphical methods. biosensor successfully applied quantification inhibitors by fixed potential amperometry, excellent detection limit values pM range. It first method reported anticancer drugs CPG77675 electrochemical assay brings several advantages over available kits high-throughput screening (HTS) namely: low cost, easy operability robustness demonstrated high reproducibility both operational storage stability, offering opportunity discover new optimize index.

Language: Английский

Simultaneous quantification of some prostaglandin analogs used in the treatment of open-angle glaucoma by RP-HPLC DOI
Miglena Smerikarova, Stanislav Bozhanov, Vania Maslarska

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113775 - 113775

Published: April 1, 2025

Language: Английский

Citations

0
Electrochemical assay for the quantification of anticancer drugs and their inhibition mechanism DOI Creative Commons
Ricardo J.B. Leote, Caroline G. Sanz, Victor C. Diculescu

et al.

Methods, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Overexpression of pyruvate kinase (PyK) is linked to many kinds malignant tumors, representing therefore one the most promising therapeutic targets for cancer treatment. Inhibition PyK slows down tumor growth or causes cell death, minimizing proliferation, and understanding inhibitor mechanism action can significantly improve therapy. The present work describes use an amperometric bienzymatic biosensor, based on oxidase (PyOx), in enzyme inhibition studies four inhibitors, CPG77675, Nilotinib, Ruxolitinib, Cerdulatinib. Their studied discussed detail, with a thorough evaluation their enzyme-inhibitor complex binding constants (Ki) concentration required 50% (IC50), employing standard procedure graphical methods. biosensor successfully applied quantification inhibitors by fixed potential amperometry, excellent detection limit values pM range. It first method reported anticancer drugs CPG77675 electrochemical assay brings several advantages over available kits high-throughput screening (HTS) namely: low cost, easy operability robustness demonstrated high reproducibility both operational storage stability, offering opportunity discover new optimize index.

Language: Английский

Citations

0